牡丹开花和衰老期间花瓣糖代谢的研究

园艺学报 ›› 2009, Vol. 36 ›› Issue (8): 1184-1190.

牡丹开花和衰老期间花瓣糖代谢的研究

史国安^1,2;郭香凤¹;张国海¹;包满珠^2*

(¹河南科技大学农学院, 洛阳市牡丹生物学重点实验室, 河南洛阳471000; ²华中农业大学园艺林学学院, 园艺植物生物学教育部重点实验室, 武汉430070)

收稿日期:2009-02-04 修回日期:2009-07-13 出版日期:2009-08-25 发布日期:2009-08-25
通讯作者: 包满珠

Analysis of Sugar Metabolism During Florescence and Flower Senescence ofTree Peony Petal

SHI Guo-an^1,2;GUO Xiang-feng¹;ZHANG Guo-hai¹;BAO Man-zhu^2*

(¹College of Agriculture, Luoyang Key Laboratory of Peony B iology, Henan University of Science and Technology, Luoyang,Henan 471000, China; ²College of Horticultural and Forestry Science, Key Laboratory of Horticultural Plant Biology, Ministry of Education, Huazhong Agricultural University,Wuhan 430070, China)

Received:2009-02-04 Revised:2009-07-13 Online:2009-08-25 Published:2009-08-25
Contact: BAO Man-zhu

摘要/Abstract

摘要： 以牡丹( Paeonia suffruticosa) 品种‘洛阳红’和‘胡红’为材料, 研究了花开放和衰老过程中花瓣可溶性糖及其代谢相关酶活性的变化。结果表明: 伴随花瓣的迅速生长展开, 总可溶性糖呈现迅速增加的趋势, 特别是己糖(葡萄糖和果糖) 含量显著增加, 盛开后己糖水平达到最高, 而蔗糖含量呈现逐渐下降的变化。己糖和蔗糖降解指数(SDI) 与花枝质量呈现极显著的正相关; 花瓣酸性转化酶活性维持较高水平, 开花过程中活性逐渐升高, 开放后逐渐下降。经主成分回归分析, 可溶性糖的代谢依赖于酸性转化酶、中性转化酶、蔗糖合成酶和蔗糖磷酸合成酶的共同作用。结果提示, 牡丹花瓣中己糖的积累在花开放和衰老过程中起着重要作用。

关键词: 牡丹, 花发育, 转化酶, 伸长生长, 糖, 代谢

Abstract: To clarify the mechanism of flower opening and senescence in tree peony ( Paeon ia suffruticosa) ‘Luoyanghong’and‘Huhong’, we investigated sugarmetabolism in the petals of two tree peony cultivars. The results showed that total soluble sugar content increased rap idlywith the cell enlargement in the petals and during flower senescence. The contents of hexoses (glucose and fructose) markedly increased during flower opening and reached a highest level at full-opening, but the content of sucrose dropped gradually. The hexose content and sucrose degradation index ( SDI) were positively correlated with the fresh weight of flower. The activity for acid invertase (AI) increased during flower opening and decreased after the flowers opened. Based on regression analyses, the soluble sugarmetabolism was related to the actions of the 4 enzymes, viz1 acid invertase (AI) , neutral invertase (NI) , sucrose synthase (SS) and sucrose phosphate synthase ( SPS). Our results suggest that hexose accumulation in the petal might be important for flowering opening and senescence in tree peony.

Key words: Paeonia suffru ticosa, flower development, invertase, elongation growth, sugar, metabolism

中图分类号:

史国安;郭香凤;张国海;包满珠. 牡丹开花和衰老期间花瓣糖代谢的研究[J]. 园艺学报, 2009, 36(8): 1184-1190.

SHI Guo-an;GUO Xiang-feng;ZHANG Guo-hai;BAO Man-zhu. Analysis of Sugar Metabolism During Florescence and Flower Senescence ofTree Peony Petal[J]. ACTA HORTICULTURAE SINICA, 2009, 36(8): 1184-1190.

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