园艺学报 ›› 2024, Vol. 51 ›› Issue (11): 2575-2593.doi: 10.16420/j.issn.0513-353x.2023-0563
王小龙1,*, 邵学东2,*, 张正文2, 钟晓敏2, 刘畅1, 王志强1, 王宝亮1, 冀晓昊1, 史祥宾1, 王海波1,**()
收稿日期:
2024-06-25
修回日期:
2024-08-16
出版日期:
2024-12-12
发布日期:
2024-11-25
通讯作者:
作者简介:
共同第一作者
基金资助:
WANG Xiaolong1, SHAO Xuedong2, ZHANG Zhengwen2, ZHONG Xiaomin2, LIU Chang1, WANG Zhiqiang1, WANG Baoliang1, JI Xiaohao1, SHI Xiangbin1, WANG Haibo1,**()
Received:
2024-06-25
Revised:
2024-08-16
Published:
2024-12-12
Online:
2024-11-25
摘要:
以山东烟台产区‘梅乐’酿酒葡萄为试材,于2019—2021年结合正交设计L16(45)‘5416’配方施肥方案,利用主成分分析法(PCA)对各施肥处理成熟期果实的百粒质量、可溶性固形物、皮果比、籽果比、籽粒数、果皮和种子(皮/籽)总酚、皮花色苷、皮/籽总黄酮、皮/籽黄烷醇、皮/籽单宁等14个品质指标进行综合评价。PCA结果表明,T11处理(N 124.5 kg · hm-2、P2O5 46.5 kg · hm-2、K2O 0 kg · hm-2、CaO 56.3 kg · hm-2和MgO 69.8 kg · hm-2)对提高‘梅乐’葡萄综合品质的效果较为明显。利用相关性分析法,确定萌芽期0 ~ 40 cm土壤N、K、Mg和成熟期P、Ca含量为花色苷的土壤营养诊断因子,确定转色期叶柄N,盛花期花序P、Zn、B,盛花期叶片K、Mo,转色期叶片Ca、Fe和Mn,成熟期叶柄Mg,成熟期果实Cu含量为花色苷的植株营养诊断因子。利用组分营养诊断法(CND)建立诊断因子与花色苷的函数模型,最终制定高花色苷葡萄园(即高质葡萄园)土壤和植株的营养诊断标准:高质园(土壤元素含量累积方差所对应的花色苷归一化值 > 0.6372,即拐点值)的土壤营养诊断标准为N 0.06 ~ 0.21 mg · g-1、P 0.08 ~ 1.17 mg · g-1、K 0.05 ~ 0.23 mg · g-1、Ca 0.52 ~ 8.51 mg · g-1和Mg 0.04 ~ 0.65 mg · g-1;高质园(植株大中量元素含量累积方差所对应的花色苷归一化值 > 0.8353;微量元素含量累积方差所对应的花色苷归一化值 > 0.8699)的植株营养诊断标准为N 5.38 ~ 11.00 mg · g-1、P 5.45 ~ 10.88 mg · g-1、K 6.73 ~ 39.75 mg · g-1、Ca 35.57 ~ 68.83 mg · g-1、Mg 9.30 ~ 24.63 mg · g-1、Fe 233.93 ~ 398.83 mg · kg-1、Mn 292.55 ~ 520.30 mg · kg-1、Zn 29.03 ~ 73.65 mg · kg-1、Cu 18.22 ~ 28.93 mg · kg-1、B 20.52 ~ 35.13 mg · kg-1和Mo 0.78 ~ 1.85 mg · kg-1;针对‘梅乐’葡萄低质园(植株大中量元素含量累积方差所对应的花色苷归一化值 < 0.8353;土壤营养元素含量累积方差所对应的花色苷归一化值 < 0.6372)的植株和土壤丰缺特点,建议采用少量多次的方式向土壤施用P肥;完善水肥管理制度,提高植株对K和Ca元素的吸收能力;通过叶面喷施适当补充缺乏的微量元素。
王小龙, 邵学东, 张正文, 钟晓敏, 刘畅, 王志强, 王宝亮, 冀晓昊, 史祥宾, 王海波. 酿酒葡萄‘梅乐’植株和土壤营养水平与果实品质的关联性分析[J]. 园艺学报, 2024, 51(11): 2575-2593.
WANG Xiaolong, SHAO Xuedong, ZHANG Zhengwen, ZHONG Xiaomin, LIU Chang, WANG Zhiqiang, WANG Baoliang, JI Xiaohao, SHI Xiangbin, WANG Haibo. Correlation Analysis of Fruit Quality and the Nutrient Level in Plant and Soil of Wine Grape‘Merlot’[J]. Acta Horticulturae Sinica, 2024, 51(11): 2575-2593.
处理代号Code | N | P2O5 | K2O | CaO | MgO | 总计Total |
---|---|---|---|---|---|---|
T1 | 0 | 0 | 0 | 0 | 0 | 0 |
T2 | 0 | 23.3 | 56.3 | 56.3 | 23.3 | 159.2 |
T3 | 0 | 46.5 | 112.5 | 112.5 | 46.5 | 318.0 |
T4 | 0 | 69.8 | 168.8 | 168.8 | 69.8 | 477.2 |
T5 | 62.3 | 0 | 56.3 | 112.5 | 69.8 | 300.9 |
T6 | 62.3 | 23.3 | 0 | 168.8 | 46.5 | 300.9 |
T7 | 62.3 | 46.5 | 168.8 | 0 | 23.3 | 300.9 |
T8 | 62.3 | 69.8 | 112.5 | 56.3 | 0 | 300.9 |
T9 | 124.5 | 0 | 112.5 | 168.8 | 23.3 | 429.1 |
T10 | 124.5 | 23.3 | 168.8 | 112.5 | 0 | 429.1 |
T11 | 124.5 | 46.5 | 0 | 56.3 | 69.8 | 297.1 |
T12 | 124.5 | 69.8 | 56.3 | 0 | 46.5 | 297.1 |
T13 | 186.8 | 0 | 168.8 | 56.3 | 46.5 | 458.4 |
T14 | 186.8 | 23.3 | 112.5 | 0 | 69.8 | 392.4 |
T15 | 186.8 | 46.5 | 56.3 | 168.8 | 0 | 458.4 |
T16 | 186.8 | 69.8 | 0 | 112.5 | 23.3 | 392.4 |
表1 田间配方施肥方案及各肥料的全年施用量
Table 1 The annual application amount of each fertilizer in each treatment of the field formula fertilization program kg · hm-2
处理代号Code | N | P2O5 | K2O | CaO | MgO | 总计Total |
---|---|---|---|---|---|---|
T1 | 0 | 0 | 0 | 0 | 0 | 0 |
T2 | 0 | 23.3 | 56.3 | 56.3 | 23.3 | 159.2 |
T3 | 0 | 46.5 | 112.5 | 112.5 | 46.5 | 318.0 |
T4 | 0 | 69.8 | 168.8 | 168.8 | 69.8 | 477.2 |
T5 | 62.3 | 0 | 56.3 | 112.5 | 69.8 | 300.9 |
T6 | 62.3 | 23.3 | 0 | 168.8 | 46.5 | 300.9 |
T7 | 62.3 | 46.5 | 168.8 | 0 | 23.3 | 300.9 |
T8 | 62.3 | 69.8 | 112.5 | 56.3 | 0 | 300.9 |
T9 | 124.5 | 0 | 112.5 | 168.8 | 23.3 | 429.1 |
T10 | 124.5 | 23.3 | 168.8 | 112.5 | 0 | 429.1 |
T11 | 124.5 | 46.5 | 0 | 56.3 | 69.8 | 297.1 |
T12 | 124.5 | 69.8 | 56.3 | 0 | 46.5 | 297.1 |
T13 | 186.8 | 0 | 168.8 | 56.3 | 46.5 | 458.4 |
T14 | 186.8 | 23.3 | 112.5 | 0 | 69.8 | 392.4 |
T15 | 186.8 | 46.5 | 56.3 | 168.8 | 0 | 458.4 |
T16 | 186.8 | 69.8 | 0 | 112.5 | 23.3 | 392.4 |
处理 Treat- ment | 年份 Year | 果汁可溶性 固形物/% Total soluble solids | 皮果比/% Peel-to-fruit ratio | 皮总酚/ (mg · g-1) Total peel phenols | 皮花色苷/ (mg · g-1) Peel anthocyanins | 皮总黄酮/ (mg · g-1) Total peel flavonoids | 皮黄烷醇/ (mg · g-1) Peel flavanols | 皮单宁/ (mg · g-1) Peel tannins |
---|---|---|---|---|---|---|---|---|
T1 | 2019 | 21.7 ± 0.4 a | 9.69 ± 0.64 defg | 7.61 ± 0.63 e | 2.06 ± 0.31 gh | 5.83 ± 0.14 cde | 39.76 ± 5.51cdef | 1.23 ± 0.07 ef |
2020 | 16.2 ± 2.1 cd | 11.23 ± 0.29 cd | 7.76 ± 0.88 b | 1.67 ± 0.25 e | 4.31 ± 0.46 d | 34.03 ± 4.68 c | 1.35 ± 0.12 cd | |
2021 | 18.9 ± 0.9 abc | 10.86 ± 1.07 bc | 3.23 ± 0.32 cde | 0.23 ± 0.02 fg | 1.68 ± 0.13 d | 12.70 ± 0.19 cd | 0.39 ± 0.04 gh | |
T2 | 2019 | 20.2 ± 2.4 a | 9.80 ± 1.10 cdefg | 12.86 ± 0.27 abcde | 3.41 ± 0.09 bcd | 9.51 ± 0.89 a | 88.52 ± 10.14 a | 3.22 ± 0.52 a |
2020 | 12.1 ± 1.1 e | 12.96 ± 0.90 b | 4.41 ± 0.55 g | 1.10 ± 0.12 f | 2.89 ± 0.33 e | 20.26 ± 2.22 f | 0.56 ± 0.03 e | |
2021 | 17.5 ± 1.9 abc | 11.75 ± 1.21 b | 2.51 ± 0.22 efg | 0.14 ± 0.01 h | 0.87 ± 0.06 f | 10.15 ± 1.34 de | 0.51 ± 0.00 fg | |
T3 | 2019 | 19.5 ± 1.4 a | 9.48 ± 0.27 efg | 9.44 ± 1.08 bcde | 2.88 ± 0.25 def | 6.27 ± 0.84 cde | 50.14 ± 2.77 b | 1.90 ± 0.27 cd |
2020 | 15.7 ± 1.7 cd | 8.84 ± 0.51 fgh | 6.62 ± 0.57 cd | 1.85 ± 0.05 e | 4.73 ± 0.48 d | 33.49 ± 2.55 c | 1.38 ± 0.07 cd | |
2021 | 17.3 ± 1.5 abc | 10.35 ± 1.71 bcd | 3.26 ± 0.18 cde | 0.23 ± 0.03 fg | 1.78 ± 0.14 d | 13.60 ± 1.63 c | 0.63 ± 0.05 de | |
T4 | 2019 | 22.8 ± 1.3 a | 11.31 ± 0.78 bc | 7.32 ± 0.49 cde | 2.52 ± 0.40 fg | 5.01 ± 0.50 ef | 38.68 ± 2.91 cdef | 1.56 ± 0.20 de |
2020 | 15.6 ± 1.6 cd | 10.58 ± 0.87 de | 6.41 ± 0.19 cde | 1.91 ± 0.13 e | 4.32 ± 0.24 d | 32.44 ± 3.88 cd | 1.19 ± 0.13 cd | |
2021 | 19.5 ± 1.6 ab | 7.31 ± 1.21 ef | 8.06 ± 1.19 a | 0.85 ± 0.09 a | 5.04 ± 0.18 a | 30.35 ± 4.23 a | 1.83 ± 0.17 a | |
T5 | 2019 | 22.0 ± 2.6 a | 10.97 ± 1.01 bcde | 5.95 ± 0.75 cde | 2.88 ± 0.38 ef | 4.45 ± 0.55 f | 31.52 ± 2.52 f | 1.09 ± 0.11 fg |
2020 | 16.3 ± 1.7 cd | 7.23 ± 1.09 h | 9.43 ± 0.45 a | 3.15 ± 0.31 ab | 6.54 ± 0.42 bc | 46.74 ± 4.76 ab | 2.02 ± 0.13 b | |
2021 | 18.4 ± 1.2 abc | 9.30 ± 0.90 cd | 2.97 ± 0.28 def | 0.30 ± 0.03 ef | 1.68 ± 0.14 d | 14.86 ± 1.43 c | 0.19 ± 0.02 k | |
T6 | 2019 | 23.1 ± 3.2 a | 10.14 ± 0.98 bcdef | 8.43 ± 0.92 abcde | 2.22 ± 0.15 gh | 5.48 ± 0.78 def | 36.20 ± 4.14 ef | 1.36 ± 0.07 ef |
2020 | 16.9 ± 0.8 bcd | 7.81 ± 0.99 gh | 7.95 ± 0.68 b | 3.43 ± 0.38 a | 7.39 ± 0.28 a | 42.80 ± 1.24 b | 1.51 ± 0.08 c | |
2021 | 19.0 ± 1.4 abc | 8.45 ± 0.86 def | 3.55 ± 0.48 cd | 0.40 ± 0.05 c | 2.32 ± 0.27 c | 13.91 ± 1.81 c | 0.37 ± 0.03 hi | |
T7 | 2019 | 20.9 ± 1.1 a | 11.49 ± 1.36 b | 11.25 ± 1.20 abcde | 3.57 ± 0.22 bc | 6.95 ± 1.02 c | 51.00 ± 3.47 b | 2.02 ± 0.07 c |
2020 | 16.6 ± 0.7 bcd | 8.97 ± 0.89 efgh | 5.29 ± 0.60 f | 2.93 ± 0.44 bc | 4.83 ± 0.38 d | 34.52 ± 4.86 c | 1.07 ± 0.13 d | |
2021 | 17.8 ± 1.3 abc | 6.88 ± 0.14 f | 3.02 ± 0.25 cdef | 0.34 ± 0.03 de | 2.42 ± 0.27 c | 14.71 ± 0.43 c | 0.50 ± 0.05 ef | |
T8 | 2019 | 22.2 ± 2.6 a | 9.60 ± 0.46 defg | 14.06 ± 1.26 a | 4.28 ± 0.15 a | 10.37 ± 1.44 a | 81.69 ± 4.83 a | 3.20 ± 0.15 a |
2020 | 18.6 ± 2.4 abc | 13.61 ± 1.58 ab | 3.94 ± 0.21 g | 1.53 ± 0.14 ef | 3.33 ± 0.40 e | 23.19 ± 3.22 f | 0.60 ± 0.05 e | |
2021 | 16.6 ± 1.3 bc | 12.03 ± 0.50 b | 2.23 ± 0.20 fg | 0.21 ± 0.03 gh | 1.32 ± 0.12 e | 9.76 ± 1.32 def | 0.32 ± 0.01 hijk | |
T9 | 2019 | 21.9 ± 2.8 a | 10.40 ± 0.66 bcdef | 10.39 ± 1.24 abcde | 3.58 ± 0.01 b | 6.44 ± 0.13 cde | 47.85 ±4.39 bc | 1.75 ± 0.08 cd |
2020 | 18.2 ± 0.8 abcd | 10.35 ± 0.72 def | 5.74 ± 0.34 ef | 2.42 ± 0.24 d | 4.97 ± 0.62 d | 31.94 ± 2.70 cde | 0.46 ± 0.04 e | |
2021 | 20.3 ± 1.2 a | 14.08 ± 1.19 a | 2.65 ± 0.06 ef | 0.36 ± 0.03 cde | 1.70 ± 0.19 d | 9.38 ± 0.92 ef | 0.32 ± 0.03 hij | |
T10 | 2019 | 22.9 ± 2.6 a | 10.36 ± 0.37 bcdef | 10.14 ± 1.10 ab | 3.35 ± 0.37 bcde | 5.25 ± 0.34 ef | 37.46 ± 3.54 def | 1.28 ± 0.08 ef |
2020 | 17.4 ± 1.4 bcd | 13.49 ± 0.15 ab | 6.04 ± 0.42 def | 1.68 ± 0.12 e | 3.44 ± 0.39 e | 26.24 ± 1.47 def | 1.17 ± 0.12 cd | |
2021 | 18.1 ± 1.5 abc | 11.85 ± 1.07 b | 3.16 ± 0.34 cde | 0.39 ± 0.03 cd | 2.18 ± 0.03 c | 12.73 ± 1.80 cd | 0.85 ± 0.13 c | |
T11 | 2019 | 22.8 ± 1.2 a | 11.06 ± 0.52 bcde | 8.78 ± 0.76 abc | 2.98 ± 0.41 def | 5.91 ± 0.33 cde | 42.86 ± 4.96 bcde | 1.92 ± 0.29 c |
2020 | 21.0 ± 2.4 a | 8.31 ± 1.26 gh | 9.24 ± 0.16 a | 3.55 ± 0.38 a | 6.44 ± 0.99 bc | 45.37 ± 2.53 ab | 3.00 ± 0.23 a | |
2021 | 19.5 ± 1.3 ab | 8.68 ± 0.89 def | 3.56 ± 0.35 cd | 0.34 ± 0.05 de | 2.30 ± 0.19 c | 14.45 ± 0.64 c | 0.75 ± 0.09 d | |
T12 | 2019 | 22.1 ± 3.6 a | 13.21 ± 1.19 a | 5.36 ± 0.57 de | 1.79 ± 0.14 h | 2.85 ± 0.28 g | 17.01 ± 1.82 g | 0.76 ± 0.07 g |
2020 | 15.4 ± 1.0 d | 8.21 ± 0.03 gh | 9.94 ± 0.23 a | 3.28 ± 0.37 ab | 7.01 ± 0.68 ab | 51.87 ± 7.24 a | 3.10 ± 0.40 a | |
2021 | 18.4 ± 1.3 abc | 10.76 ± 0.81 bc | 3.22 ± 0.23 cde | 0.32 ± 0.03 ef | 1.79 ± 0.13 d | 11.97 ± 1.16 cde | 0.24 ± 0.01 jk | |
T13 | 2019 | 20.0 ± 1.8 a | 9.20 ± 0.29 fg | 12.44 ± 1.49 abc | 3.54 ± 0.23 bc | 6.84 ± 0.93 cd | 45.64 ± 0.99 bcd | 2.48 ± 0.13 b |
2020 | 16.8 ± 0.1 bcd | 8.32 ± 0.88 gh | 7.17 ± 0.29 bc | 2.41 ± 0.28 d | 5.85 ± 0.36 c | 43.14 ± 4.69 b | 2.95 ± 0.19 a | |
2021 | 15.9 ± 2.2 c | 9.06 ± 1.25 cde | 3.52 ± 0.38 cd | 0.18 ± 0.02 gh | 1.69 ± 0.21 d | 12.15 ± 1.13 cde | 0.72 ± 0.07 d | |
T14 | 2019 | 21.9 ± 1.3 a | 11.19 ± 1.04 bcd | 9.51 ± 1.07 abcd | 3.07 ± 0.15 cde | 5.10 ± 0.52 ef | 34.37 ± 3.28 ef | 1.21 ± 0.08 ef |
2020 | 16.6 ± 1.2 bcd | 12.30 ± 1.73 bc | 5.51 ± 0.29 f | 1.93 ± 0.17 e | 4.29 ± 0.32 d | 33.49 ± 1.50 c | 1.89 ± 0.14 b | |
2021 | 17.8 ± 2.1 abc | 15.52 ± 1.46 a | 1.81 ± 0.25 g | 0.24 ± 0.02 fg | 1.57 ± 0.18 de | 7.05 ± 0.49 f | 0.26 ± 0.02 ijk | |
T15 | 2019 | 22.7 ± 1.1 a | 10.51 ± 1.05 bcdef | 10.17 ± 1.51 abcde | 3.20 ± 0.25 bcde | 5.98 ± 0.50 cde | 42.49 ± 5.69 bcde | 1.95 ± 0.19 c |
2020 | 15.4 ± 2.2 d | 15.04 ± 1.14 a | 4.45 ± 0.46 g | 1.15 ± 0.12 f | 2.95 ± 0.04 e | 25.56 ± 2.91 ef | 1.03 ± 0.04 d | |
2021 | 18.9 ± 2.2 abc | 6.90 ± 0.24 f | 5.72 ± 0.66 b | 0.63 ± 0.05 b | 3.65 ± 0.33 b | 19.70 ± 2.22 b | 1.18 ± 0.12 b | |
T16 | 2019 | 22.8 ± 2.3 a | 8.41 ± 0.35 g | 12.59 ± 0.87 ab | 4.42 ± 0.33 a | 8.19 ± 1.11 b | 83.83 ± 8.56 a | 2.86 ± 0.30 a |
2020 | 19.5 ± 1.9 ab | 9.47 ± 0.62 efg | 9.14 ± 0.18 a | 2.54 ± 0.25 cd | 6.33 ± 0.21 bc | 49.61 ± 3.64 ab | 2.86 ± 0.29 a | |
2021 | 19.1 ± 2.5 abc | 11.50 ± 1.28 b | 3.83 ± 0.32 c | 0.34 ± 0.03 de | 2.29 ± 0.26 c | 15.04 ± 1.36 c | 0.92 ± 0.01 c |
表2 不同施肥处理各果实品质(果汁和果皮)
Table 2 Fruit quality(total soluble solids and peel)under different fertilization treatments
处理 Treat- ment | 年份 Year | 果汁可溶性 固形物/% Total soluble solids | 皮果比/% Peel-to-fruit ratio | 皮总酚/ (mg · g-1) Total peel phenols | 皮花色苷/ (mg · g-1) Peel anthocyanins | 皮总黄酮/ (mg · g-1) Total peel flavonoids | 皮黄烷醇/ (mg · g-1) Peel flavanols | 皮单宁/ (mg · g-1) Peel tannins |
---|---|---|---|---|---|---|---|---|
T1 | 2019 | 21.7 ± 0.4 a | 9.69 ± 0.64 defg | 7.61 ± 0.63 e | 2.06 ± 0.31 gh | 5.83 ± 0.14 cde | 39.76 ± 5.51cdef | 1.23 ± 0.07 ef |
2020 | 16.2 ± 2.1 cd | 11.23 ± 0.29 cd | 7.76 ± 0.88 b | 1.67 ± 0.25 e | 4.31 ± 0.46 d | 34.03 ± 4.68 c | 1.35 ± 0.12 cd | |
2021 | 18.9 ± 0.9 abc | 10.86 ± 1.07 bc | 3.23 ± 0.32 cde | 0.23 ± 0.02 fg | 1.68 ± 0.13 d | 12.70 ± 0.19 cd | 0.39 ± 0.04 gh | |
T2 | 2019 | 20.2 ± 2.4 a | 9.80 ± 1.10 cdefg | 12.86 ± 0.27 abcde | 3.41 ± 0.09 bcd | 9.51 ± 0.89 a | 88.52 ± 10.14 a | 3.22 ± 0.52 a |
2020 | 12.1 ± 1.1 e | 12.96 ± 0.90 b | 4.41 ± 0.55 g | 1.10 ± 0.12 f | 2.89 ± 0.33 e | 20.26 ± 2.22 f | 0.56 ± 0.03 e | |
2021 | 17.5 ± 1.9 abc | 11.75 ± 1.21 b | 2.51 ± 0.22 efg | 0.14 ± 0.01 h | 0.87 ± 0.06 f | 10.15 ± 1.34 de | 0.51 ± 0.00 fg | |
T3 | 2019 | 19.5 ± 1.4 a | 9.48 ± 0.27 efg | 9.44 ± 1.08 bcde | 2.88 ± 0.25 def | 6.27 ± 0.84 cde | 50.14 ± 2.77 b | 1.90 ± 0.27 cd |
2020 | 15.7 ± 1.7 cd | 8.84 ± 0.51 fgh | 6.62 ± 0.57 cd | 1.85 ± 0.05 e | 4.73 ± 0.48 d | 33.49 ± 2.55 c | 1.38 ± 0.07 cd | |
2021 | 17.3 ± 1.5 abc | 10.35 ± 1.71 bcd | 3.26 ± 0.18 cde | 0.23 ± 0.03 fg | 1.78 ± 0.14 d | 13.60 ± 1.63 c | 0.63 ± 0.05 de | |
T4 | 2019 | 22.8 ± 1.3 a | 11.31 ± 0.78 bc | 7.32 ± 0.49 cde | 2.52 ± 0.40 fg | 5.01 ± 0.50 ef | 38.68 ± 2.91 cdef | 1.56 ± 0.20 de |
2020 | 15.6 ± 1.6 cd | 10.58 ± 0.87 de | 6.41 ± 0.19 cde | 1.91 ± 0.13 e | 4.32 ± 0.24 d | 32.44 ± 3.88 cd | 1.19 ± 0.13 cd | |
2021 | 19.5 ± 1.6 ab | 7.31 ± 1.21 ef | 8.06 ± 1.19 a | 0.85 ± 0.09 a | 5.04 ± 0.18 a | 30.35 ± 4.23 a | 1.83 ± 0.17 a | |
T5 | 2019 | 22.0 ± 2.6 a | 10.97 ± 1.01 bcde | 5.95 ± 0.75 cde | 2.88 ± 0.38 ef | 4.45 ± 0.55 f | 31.52 ± 2.52 f | 1.09 ± 0.11 fg |
2020 | 16.3 ± 1.7 cd | 7.23 ± 1.09 h | 9.43 ± 0.45 a | 3.15 ± 0.31 ab | 6.54 ± 0.42 bc | 46.74 ± 4.76 ab | 2.02 ± 0.13 b | |
2021 | 18.4 ± 1.2 abc | 9.30 ± 0.90 cd | 2.97 ± 0.28 def | 0.30 ± 0.03 ef | 1.68 ± 0.14 d | 14.86 ± 1.43 c | 0.19 ± 0.02 k | |
T6 | 2019 | 23.1 ± 3.2 a | 10.14 ± 0.98 bcdef | 8.43 ± 0.92 abcde | 2.22 ± 0.15 gh | 5.48 ± 0.78 def | 36.20 ± 4.14 ef | 1.36 ± 0.07 ef |
2020 | 16.9 ± 0.8 bcd | 7.81 ± 0.99 gh | 7.95 ± 0.68 b | 3.43 ± 0.38 a | 7.39 ± 0.28 a | 42.80 ± 1.24 b | 1.51 ± 0.08 c | |
2021 | 19.0 ± 1.4 abc | 8.45 ± 0.86 def | 3.55 ± 0.48 cd | 0.40 ± 0.05 c | 2.32 ± 0.27 c | 13.91 ± 1.81 c | 0.37 ± 0.03 hi | |
T7 | 2019 | 20.9 ± 1.1 a | 11.49 ± 1.36 b | 11.25 ± 1.20 abcde | 3.57 ± 0.22 bc | 6.95 ± 1.02 c | 51.00 ± 3.47 b | 2.02 ± 0.07 c |
2020 | 16.6 ± 0.7 bcd | 8.97 ± 0.89 efgh | 5.29 ± 0.60 f | 2.93 ± 0.44 bc | 4.83 ± 0.38 d | 34.52 ± 4.86 c | 1.07 ± 0.13 d | |
2021 | 17.8 ± 1.3 abc | 6.88 ± 0.14 f | 3.02 ± 0.25 cdef | 0.34 ± 0.03 de | 2.42 ± 0.27 c | 14.71 ± 0.43 c | 0.50 ± 0.05 ef | |
T8 | 2019 | 22.2 ± 2.6 a | 9.60 ± 0.46 defg | 14.06 ± 1.26 a | 4.28 ± 0.15 a | 10.37 ± 1.44 a | 81.69 ± 4.83 a | 3.20 ± 0.15 a |
2020 | 18.6 ± 2.4 abc | 13.61 ± 1.58 ab | 3.94 ± 0.21 g | 1.53 ± 0.14 ef | 3.33 ± 0.40 e | 23.19 ± 3.22 f | 0.60 ± 0.05 e | |
2021 | 16.6 ± 1.3 bc | 12.03 ± 0.50 b | 2.23 ± 0.20 fg | 0.21 ± 0.03 gh | 1.32 ± 0.12 e | 9.76 ± 1.32 def | 0.32 ± 0.01 hijk | |
T9 | 2019 | 21.9 ± 2.8 a | 10.40 ± 0.66 bcdef | 10.39 ± 1.24 abcde | 3.58 ± 0.01 b | 6.44 ± 0.13 cde | 47.85 ±4.39 bc | 1.75 ± 0.08 cd |
2020 | 18.2 ± 0.8 abcd | 10.35 ± 0.72 def | 5.74 ± 0.34 ef | 2.42 ± 0.24 d | 4.97 ± 0.62 d | 31.94 ± 2.70 cde | 0.46 ± 0.04 e | |
2021 | 20.3 ± 1.2 a | 14.08 ± 1.19 a | 2.65 ± 0.06 ef | 0.36 ± 0.03 cde | 1.70 ± 0.19 d | 9.38 ± 0.92 ef | 0.32 ± 0.03 hij | |
T10 | 2019 | 22.9 ± 2.6 a | 10.36 ± 0.37 bcdef | 10.14 ± 1.10 ab | 3.35 ± 0.37 bcde | 5.25 ± 0.34 ef | 37.46 ± 3.54 def | 1.28 ± 0.08 ef |
2020 | 17.4 ± 1.4 bcd | 13.49 ± 0.15 ab | 6.04 ± 0.42 def | 1.68 ± 0.12 e | 3.44 ± 0.39 e | 26.24 ± 1.47 def | 1.17 ± 0.12 cd | |
2021 | 18.1 ± 1.5 abc | 11.85 ± 1.07 b | 3.16 ± 0.34 cde | 0.39 ± 0.03 cd | 2.18 ± 0.03 c | 12.73 ± 1.80 cd | 0.85 ± 0.13 c | |
T11 | 2019 | 22.8 ± 1.2 a | 11.06 ± 0.52 bcde | 8.78 ± 0.76 abc | 2.98 ± 0.41 def | 5.91 ± 0.33 cde | 42.86 ± 4.96 bcde | 1.92 ± 0.29 c |
2020 | 21.0 ± 2.4 a | 8.31 ± 1.26 gh | 9.24 ± 0.16 a | 3.55 ± 0.38 a | 6.44 ± 0.99 bc | 45.37 ± 2.53 ab | 3.00 ± 0.23 a | |
2021 | 19.5 ± 1.3 ab | 8.68 ± 0.89 def | 3.56 ± 0.35 cd | 0.34 ± 0.05 de | 2.30 ± 0.19 c | 14.45 ± 0.64 c | 0.75 ± 0.09 d | |
T12 | 2019 | 22.1 ± 3.6 a | 13.21 ± 1.19 a | 5.36 ± 0.57 de | 1.79 ± 0.14 h | 2.85 ± 0.28 g | 17.01 ± 1.82 g | 0.76 ± 0.07 g |
2020 | 15.4 ± 1.0 d | 8.21 ± 0.03 gh | 9.94 ± 0.23 a | 3.28 ± 0.37 ab | 7.01 ± 0.68 ab | 51.87 ± 7.24 a | 3.10 ± 0.40 a | |
2021 | 18.4 ± 1.3 abc | 10.76 ± 0.81 bc | 3.22 ± 0.23 cde | 0.32 ± 0.03 ef | 1.79 ± 0.13 d | 11.97 ± 1.16 cde | 0.24 ± 0.01 jk | |
T13 | 2019 | 20.0 ± 1.8 a | 9.20 ± 0.29 fg | 12.44 ± 1.49 abc | 3.54 ± 0.23 bc | 6.84 ± 0.93 cd | 45.64 ± 0.99 bcd | 2.48 ± 0.13 b |
2020 | 16.8 ± 0.1 bcd | 8.32 ± 0.88 gh | 7.17 ± 0.29 bc | 2.41 ± 0.28 d | 5.85 ± 0.36 c | 43.14 ± 4.69 b | 2.95 ± 0.19 a | |
2021 | 15.9 ± 2.2 c | 9.06 ± 1.25 cde | 3.52 ± 0.38 cd | 0.18 ± 0.02 gh | 1.69 ± 0.21 d | 12.15 ± 1.13 cde | 0.72 ± 0.07 d | |
T14 | 2019 | 21.9 ± 1.3 a | 11.19 ± 1.04 bcd | 9.51 ± 1.07 abcd | 3.07 ± 0.15 cde | 5.10 ± 0.52 ef | 34.37 ± 3.28 ef | 1.21 ± 0.08 ef |
2020 | 16.6 ± 1.2 bcd | 12.30 ± 1.73 bc | 5.51 ± 0.29 f | 1.93 ± 0.17 e | 4.29 ± 0.32 d | 33.49 ± 1.50 c | 1.89 ± 0.14 b | |
2021 | 17.8 ± 2.1 abc | 15.52 ± 1.46 a | 1.81 ± 0.25 g | 0.24 ± 0.02 fg | 1.57 ± 0.18 de | 7.05 ± 0.49 f | 0.26 ± 0.02 ijk | |
T15 | 2019 | 22.7 ± 1.1 a | 10.51 ± 1.05 bcdef | 10.17 ± 1.51 abcde | 3.20 ± 0.25 bcde | 5.98 ± 0.50 cde | 42.49 ± 5.69 bcde | 1.95 ± 0.19 c |
2020 | 15.4 ± 2.2 d | 15.04 ± 1.14 a | 4.45 ± 0.46 g | 1.15 ± 0.12 f | 2.95 ± 0.04 e | 25.56 ± 2.91 ef | 1.03 ± 0.04 d | |
2021 | 18.9 ± 2.2 abc | 6.90 ± 0.24 f | 5.72 ± 0.66 b | 0.63 ± 0.05 b | 3.65 ± 0.33 b | 19.70 ± 2.22 b | 1.18 ± 0.12 b | |
T16 | 2019 | 22.8 ± 2.3 a | 8.41 ± 0.35 g | 12.59 ± 0.87 ab | 4.42 ± 0.33 a | 8.19 ± 1.11 b | 83.83 ± 8.56 a | 2.86 ± 0.30 a |
2020 | 19.5 ± 1.9 ab | 9.47 ± 0.62 efg | 9.14 ± 0.18 a | 2.54 ± 0.25 cd | 6.33 ± 0.21 bc | 49.61 ± 3.64 ab | 2.86 ± 0.29 a | |
2021 | 19.1 ± 2.5 abc | 11.50 ± 1.28 b | 3.83 ± 0.32 c | 0.34 ± 0.03 de | 2.29 ± 0.26 c | 15.04 ± 1.36 c | 0.92 ± 0.01 c |
处理 Treat- ment | 年份 Year | 百粒质量/g 100 grain weight | 籽果比/% Seed-to-fruit ratio | 籽粒数 Number of seeds | 籽总酚/ (mg · g-1) Total seed phenols | 籽总黄酮/ (mg · g-1) Total seed flavonoids | 籽黄烷醇/ (mg · g-1) Seed flavanols | 籽单宁/ (mg · g-1) Seed tannins |
---|---|---|---|---|---|---|---|---|
T1 | 2019 | 156.5 ± 21.9 abc | 5.03 ± 0.35 cde | 62 ± 6 a | 18.68 ± 0.78 f | 27.03 ± 1.35 f | 286.19 ± 15.73 ef | 7.01 ± 0.75 d |
2020 | 146.7 ± 12.0 de | 6.28 ± 0.72 bc | 65 ± 3 abc | 9.43 ± 0.96 h | 14.27 ± 0.90 e | 208.61 ± 12.49 hi | 1.65 ± 0.05 i | |
2021 | 158.2 ± 23.5 bcd | 6.02 ± 0.25 ab | 76 ± 7 ab | 26.15 ± 3.57 cde | 18.35 ± 0.52 efg | 361.54 ± 28.28 cdef | 17.23 ± 0.74 ef | |
T2 | 2019 | 160.5 ± 17.5 ab | 4.78 ± 0.20 efg | 56 ± 2 ab | 13.42 ± 0.20 gh | 22.61 ± 0.86 fg | 267.16 ± 20.31 f | 2.07 ± 0.16 g |
2020 | 170.0 ± 25.8 bcd | 5.70 ± 0.50 cdef | 64 ± 6 abc | 6.73 ± 0.56 h | 10.4 6 ± 1.11 f | 149.30 ± 13.43 j | 2.15 ± 0.26 hi | |
2021 | 166.3 ± 13.0 abcd | 5.52 ± 0.57 ab | 68 ± 8 ab | 25.39 ± 0.86 cde | 23.61 ± 1.01 bcd | 363.78 ± 28.33 cdef | 18.14 ± 1.92 cdef | |
T3 | 2019 | 165.8 ± 9.2 a | 4.35 ± 0.09 efg | 54 ± 5 ab | 32.32 ± 3.26 ab | 66.51 ± 4.57 b | 647.25 ± 53.11 a | 8.43 ± 0.50 c |
2020 | 199.2 ± 27.2 abc | 5.12 ± 0.23 def | 73 ± 4 abc | 18.15 ± 2.66 d | 21.66 ± 0.23 d | 291.15 ± 20.86 fg | 7.31 ± 0.74 d | |
2021 | 180.2 ± 13.4 abcd | 5.18 ± 0.48 b | 68 ± 4 ab | 33.00 ± 2.87 a | 28.89 ± 4.17 a | 485.17 ± 55.54 a | 22.22 ± 1.90 a | |
T4 | 2019 | 164.3 ± 21.3 a | 4.99 ± 0.56 cdef | 59 ± 4 ab | 17.08 ± 1.58 fg | 39.08 ± 1.66 de | 398.30 ± 16.84 cd | 4.64 ± 0.52 f |
2020 | 168.7 ± 19.7 bcd | 5.98 ± 0.25 cde | 69 ± 11 abc | 13.06 ± 0.95 g | 16.57 ± 1.42 e | 207.94 ± 25.84 hi | 5.48 ± 0.32 ef | |
2021 | 162.2 ± 7.9 abcd | 6.38 ± 0.13 ab | 79 ± 9 ab | 22.29 ± 2.31 de | 17.56 ± 1.24 g | 302.39 ± 21.47 fg | 15.53 ± 1.64 f | |
T5 | 2019 | 144.3 ± 7.2 abcd | 5.87 ± 0.24 ab | 62 ± 4 a | 10.62 ± 0.59 h | 40.01 ± 5.05 de | 115.87 ± 5.61 g | 2.71 ± 0.24 g |
2020 | 165.4 ± 18.9 bcd | 5.61 ± 0.32 cdef | 72 ± 7 abc | 22.44 ± 0.90 bc | 32.74 ± 2.92 b | 407.32 ± 53.68 bc | 11.73 ± 0.95 a | |
2021 | 154.2 ± 13.9 cd | 6.06 ± 0.96 ab | 71 ± 4 ab | 28.50 ± 2.19 abc | 21.88 ± 0.14 cdef | 388.64 ± 33.92 bcd | 17.59 ± 2.34 def | |
T6 | 2019 | 143.6 ± 11.2 abcd | 4.94 ± 0.52 defg | 54 ± 4 ab | 26.76 ± 3.17 cd | 46.83 ± 2.22 cd | 454.25 ± 49.64 c | 6.00 ± 0.76 e |
2020 | 191.5 ± 4.2 abc | 5.42 ± 0.61 cdef | 75 ± 10 ab | 21.92 ± 2.94 bc | 32.72 ± 3.85 b | 389.97 ± 26.79 cd | 10.91 ± 1.17 ab | |
2021 | 148 ± 15.6 cd | 6.53 ± 0.41 a | 75 ± 5 ab | 29.54 ± 3.01 abc | 20.15 ± 3.17 defg | 385.65 ± 26.85 bcd | 20.74 ± 1.92 abcd | |
T7 | 2019 | 157.3 ± 15.9 ab | 4.54 ± 0.48 efg | 50 ± 7 b | 25.01 ± 1.16 de | 49.17 ± 5.35 c | 467.09 ± 62.30 c | 6.72 ± 0.55 de |
2020 | 216.4 ± 29.7 a | 4.81 ± 0.64 f | 71 ± 7 abc | 16.86 ± 0.38 def | 28.41 ± 3.54 c | 308.00 ± 10.04 efg | 8.74 ± 0.94 c | |
2021 | 193.7 ± 13.6 ab | 5.25 ± 0.72 b | 73 ± 6 ab | 25.31 ± 2.15 cde | 18.19 ± 0.71 efg | 327.89 ± 21.23 defg | 18.63 ± 0.36 bcdef | |
T8 | 2019 | 170.5 ± 20.8 a | 4.18 ± 0.13 fg | 51 ± 6 b | 25.60 ± 2.57 de | 62.71 ± 6.07 b | 546.65 ± 73.47 b | 8.95 ± 0.29 c |
2020 | 194.9 ± 14.9 abc | 4.81 ± 0.72 f | 60 ± 4 c | 18.11 ± 1.52 d | 26.57 ± 2.81 c | 278.73 ± 47.24 g | 6.49 ± 0.51 de | |
2021 | 196.5 ± 26.0 a | 5.43 ± 0.71 ab | 73 ± 8 ab | 27.53 ± 3.58 bc | 28.78 ± 3.14 a | 389.49 ± 38.64 bcd | 16.69 ± 1.41 ef | |
T9 | 2019 | 148.9 ± 14.2 abcd | 5.07 ± 0.65 bcde | 61 ± 6 a | 30.09 ± 3.49 bc | 70.67 ± 11.11 b | 589.29 ± 22.68 ab | 10.03 ± 0.62 b |
2020 | 164.9 ± 16.1 bcd | 6.06 ± 0.34 bcd | 67 ± 11 abc | 14.02 ± 0.93 efg | 22.58 ± 2.25 d | 251.58 ± 10.24 gh | 6.49 ± 0.63 de | |
2021 | 182.5 ± 24.2 abc | 6.10 ± 0.69 ab | 77 ± 4 ab | 29.83 ± 0.94 abc | 26.41 ± 3.09 ab | 386.35 ± 29.42 bcd | 20.71 ± 1.04 abcd | |
T10 | 2019 | 149.9 ± 18.6 abcd | 5.71 ± 0.41 abcd | 62 ± 3 a | 12.70 ± 1.36 h | 15.44 ± 0.96 g | 223.24 ± 10.64 f | 2.10 ± 0.19 g |
2020 | 161.9 ± 21.0 cd | 6.96 ± 0.78 b | 72 ± 2 abc | 13.96 ± 1.80 efg | 15.45 ± 1.64 e | 186.97 ± 15.60 ij | 3.26 ± 0.35 gh | |
2021 | 176.5 ± 20.7 abcd | 6.54 ± 0.49 a | 81 ± 6 a | 28.61 ± 1.76 abc | 23.20 ± 2.46 bcd | 407.08 ± 51.68 bc | 21.42 ± 1.95 ab | |
T11 | 2019 | 149.8 ± 7.8 abcd | 6.12 ± 0.62 a | 59 ± 6 ab | 13.29 ± 0.50 gh | 25.80 ± 3.02 f | 289.72 ± 43.97 ef | 2.71 ± 0.43 g |
2020 | 186.9 ± 20.5 abc | 5.96 ± 0.34 cde | 78 ± 6 a | 27.19 ± 3.06 a | 36.76 ± 3.19 a | 453.57 ± 50.83 ab | 12.26 ± 1.16 a | |
2021 | 182.3 ± 9.0 abc | 6.12 ± 0.85 ab | 81 ± 10 a | 29.85 ± 3.20 abc | 25.21 ± 1.85 abc | 447.84 ± 43.60 ab | 19.55 ± 2.87 abcde | |
T12 | 2019 | 149.4 ± 10.5 abcd | 5.00 ± 0.41 cdef | 56 ± 7 ab | 25.23 ± 3.40 de | 54.04 ± 5.07 c | 456.57 ± 42.23 c | 8.22 ± 0.79 c |
2020 | 169.1 ± 27.9 bcd | 5.05 ± 0.14 def | 67 ± 7 abc | 25.29 ± 4.21 ab | 30.32 ± 1.71 bc | 342.48 ± 48.74 def | 9.65 ± 0.88 bc | |
2021 | 170.3 ± 22.6 abcd | 5.97 ± 0.56 ab | 76 ± 8 ab | 27.04 ± 3.05 bcd | 22.20 ± 3.03 cde | 369.72 ± 21.61 cde | 18.77 ± 0.81 bcde | |
T13 | 2019 | 126.6 ± 3.6 d | 4.90 ± 0.23 defg | 51 ± 3 b | 11.42 ± 1.33 h | 19.03 ± 0.41 fg | 236.65 ± 35.58 f | 2.35 ± 0.18 g |
2020 | 201.6 ± 1.7 ab | 4.94 ± 0.75 ef | 68 ± 11 abc | 19.40 ± 2.94 cd | 26.93 ± 3.24 c | 350.39 ± 33.20 de | 10.89 ± 1.41 ab | |
2021 | 175.2 ± 20.5 abcd | 5.22 ± 0.48 b | 66 ± 6 b | 21.94 ± 2.02 e | 17.21 ± 2.03 g | 287.68 ± 17.83 g | 18.18 ± 2.15 bcdef | |
T14 | 2019 | 128.9 ± 4.8 cd | 4.12 ± 0.52 fg | 54 ± 2 ab | 22.48 ± 3.16 e | 36.06 ± 4.56 e | 348.04 ± 32.39 de | 4.91 ± 0.30 f |
2020 | 185.2 ± 14.8 abc | 5.53 ± 0.66 cdef | 63 ± 5 bc | 17.52 ± 1.26 de | 10.04 ± 1.15 f | 133.52 ± 14.90 j | 1.79 ± 0.15 i | |
2021 | 146 ± 16.1 d | 6.27 ± 0.69 ab | 69 ± 2 ab | 31.50 ± 4.55 ab | 24.45 ± 2.29 bc | 421.91 ± 28.79 bc | 22.01 ± 2.02 a | |
T15 | 2019 | 149.4 ± 14.2 abcd | 5.07 ± 0.44 bcde | 62 ± 5 a | 34.74 ± 3.25 a | 81.68 ± 5.21 a | 628.15 ± 78.85 a | 12.73 ± 1.30 a |
2020 | 125.6 ± 13.8 e | 8.28 ± 0.21 a | 65 ± 7 abc | 13.63 ± 1.13 fg | 14.61 ± 0.95 e | 183.74 ± 14.08 ij | 4.56 ± 0.45 fg | |
2021 | 162.0 ± 13.8 abcd | 6.18 ± 0.04 ab | 75 ± 9 ab | 25.08 ± 1.41 cde | 17.76 ± 1.04 fg | 310.56 ± 27.31 efg | 18.76 ± 0.59 bcde | |
T16 | 2019 | 133.7 ± 12.0 bcd | 5.79 ± 0.69 abc | 59 ± 5 ab | 11.88 ± 1.10 h | 24.80 ± 2.37 f | 293.20 ± 29.64 ef | 2.16 ± 0.19 g |
2020 | 191.3 ± 7.5 abc | 5.23 ± 0.62 def | 72 ± 4 abc | 26.54 ± 0.52 a | 29.47 ± 0.45 bc | 466.62 ± 44.12 a | 10.12 ± 1.19 bc | |
2021 | 159.5 ± 23.8 bcd | 5.85 ± 0.72 ab | 68 ± 10 ab | 28.88 ± 2.59 abc | 19.95 ± 0.58 defg | 377.95 ± 35.56cd | 20.88 ± 0.15 abc |
表3 不同施肥处理各果实品质(籽)
Table 3 Fruit quality(seed)under different fertilization treatments
处理 Treat- ment | 年份 Year | 百粒质量/g 100 grain weight | 籽果比/% Seed-to-fruit ratio | 籽粒数 Number of seeds | 籽总酚/ (mg · g-1) Total seed phenols | 籽总黄酮/ (mg · g-1) Total seed flavonoids | 籽黄烷醇/ (mg · g-1) Seed flavanols | 籽单宁/ (mg · g-1) Seed tannins |
---|---|---|---|---|---|---|---|---|
T1 | 2019 | 156.5 ± 21.9 abc | 5.03 ± 0.35 cde | 62 ± 6 a | 18.68 ± 0.78 f | 27.03 ± 1.35 f | 286.19 ± 15.73 ef | 7.01 ± 0.75 d |
2020 | 146.7 ± 12.0 de | 6.28 ± 0.72 bc | 65 ± 3 abc | 9.43 ± 0.96 h | 14.27 ± 0.90 e | 208.61 ± 12.49 hi | 1.65 ± 0.05 i | |
2021 | 158.2 ± 23.5 bcd | 6.02 ± 0.25 ab | 76 ± 7 ab | 26.15 ± 3.57 cde | 18.35 ± 0.52 efg | 361.54 ± 28.28 cdef | 17.23 ± 0.74 ef | |
T2 | 2019 | 160.5 ± 17.5 ab | 4.78 ± 0.20 efg | 56 ± 2 ab | 13.42 ± 0.20 gh | 22.61 ± 0.86 fg | 267.16 ± 20.31 f | 2.07 ± 0.16 g |
2020 | 170.0 ± 25.8 bcd | 5.70 ± 0.50 cdef | 64 ± 6 abc | 6.73 ± 0.56 h | 10.4 6 ± 1.11 f | 149.30 ± 13.43 j | 2.15 ± 0.26 hi | |
2021 | 166.3 ± 13.0 abcd | 5.52 ± 0.57 ab | 68 ± 8 ab | 25.39 ± 0.86 cde | 23.61 ± 1.01 bcd | 363.78 ± 28.33 cdef | 18.14 ± 1.92 cdef | |
T3 | 2019 | 165.8 ± 9.2 a | 4.35 ± 0.09 efg | 54 ± 5 ab | 32.32 ± 3.26 ab | 66.51 ± 4.57 b | 647.25 ± 53.11 a | 8.43 ± 0.50 c |
2020 | 199.2 ± 27.2 abc | 5.12 ± 0.23 def | 73 ± 4 abc | 18.15 ± 2.66 d | 21.66 ± 0.23 d | 291.15 ± 20.86 fg | 7.31 ± 0.74 d | |
2021 | 180.2 ± 13.4 abcd | 5.18 ± 0.48 b | 68 ± 4 ab | 33.00 ± 2.87 a | 28.89 ± 4.17 a | 485.17 ± 55.54 a | 22.22 ± 1.90 a | |
T4 | 2019 | 164.3 ± 21.3 a | 4.99 ± 0.56 cdef | 59 ± 4 ab | 17.08 ± 1.58 fg | 39.08 ± 1.66 de | 398.30 ± 16.84 cd | 4.64 ± 0.52 f |
2020 | 168.7 ± 19.7 bcd | 5.98 ± 0.25 cde | 69 ± 11 abc | 13.06 ± 0.95 g | 16.57 ± 1.42 e | 207.94 ± 25.84 hi | 5.48 ± 0.32 ef | |
2021 | 162.2 ± 7.9 abcd | 6.38 ± 0.13 ab | 79 ± 9 ab | 22.29 ± 2.31 de | 17.56 ± 1.24 g | 302.39 ± 21.47 fg | 15.53 ± 1.64 f | |
T5 | 2019 | 144.3 ± 7.2 abcd | 5.87 ± 0.24 ab | 62 ± 4 a | 10.62 ± 0.59 h | 40.01 ± 5.05 de | 115.87 ± 5.61 g | 2.71 ± 0.24 g |
2020 | 165.4 ± 18.9 bcd | 5.61 ± 0.32 cdef | 72 ± 7 abc | 22.44 ± 0.90 bc | 32.74 ± 2.92 b | 407.32 ± 53.68 bc | 11.73 ± 0.95 a | |
2021 | 154.2 ± 13.9 cd | 6.06 ± 0.96 ab | 71 ± 4 ab | 28.50 ± 2.19 abc | 21.88 ± 0.14 cdef | 388.64 ± 33.92 bcd | 17.59 ± 2.34 def | |
T6 | 2019 | 143.6 ± 11.2 abcd | 4.94 ± 0.52 defg | 54 ± 4 ab | 26.76 ± 3.17 cd | 46.83 ± 2.22 cd | 454.25 ± 49.64 c | 6.00 ± 0.76 e |
2020 | 191.5 ± 4.2 abc | 5.42 ± 0.61 cdef | 75 ± 10 ab | 21.92 ± 2.94 bc | 32.72 ± 3.85 b | 389.97 ± 26.79 cd | 10.91 ± 1.17 ab | |
2021 | 148 ± 15.6 cd | 6.53 ± 0.41 a | 75 ± 5 ab | 29.54 ± 3.01 abc | 20.15 ± 3.17 defg | 385.65 ± 26.85 bcd | 20.74 ± 1.92 abcd | |
T7 | 2019 | 157.3 ± 15.9 ab | 4.54 ± 0.48 efg | 50 ± 7 b | 25.01 ± 1.16 de | 49.17 ± 5.35 c | 467.09 ± 62.30 c | 6.72 ± 0.55 de |
2020 | 216.4 ± 29.7 a | 4.81 ± 0.64 f | 71 ± 7 abc | 16.86 ± 0.38 def | 28.41 ± 3.54 c | 308.00 ± 10.04 efg | 8.74 ± 0.94 c | |
2021 | 193.7 ± 13.6 ab | 5.25 ± 0.72 b | 73 ± 6 ab | 25.31 ± 2.15 cde | 18.19 ± 0.71 efg | 327.89 ± 21.23 defg | 18.63 ± 0.36 bcdef | |
T8 | 2019 | 170.5 ± 20.8 a | 4.18 ± 0.13 fg | 51 ± 6 b | 25.60 ± 2.57 de | 62.71 ± 6.07 b | 546.65 ± 73.47 b | 8.95 ± 0.29 c |
2020 | 194.9 ± 14.9 abc | 4.81 ± 0.72 f | 60 ± 4 c | 18.11 ± 1.52 d | 26.57 ± 2.81 c | 278.73 ± 47.24 g | 6.49 ± 0.51 de | |
2021 | 196.5 ± 26.0 a | 5.43 ± 0.71 ab | 73 ± 8 ab | 27.53 ± 3.58 bc | 28.78 ± 3.14 a | 389.49 ± 38.64 bcd | 16.69 ± 1.41 ef | |
T9 | 2019 | 148.9 ± 14.2 abcd | 5.07 ± 0.65 bcde | 61 ± 6 a | 30.09 ± 3.49 bc | 70.67 ± 11.11 b | 589.29 ± 22.68 ab | 10.03 ± 0.62 b |
2020 | 164.9 ± 16.1 bcd | 6.06 ± 0.34 bcd | 67 ± 11 abc | 14.02 ± 0.93 efg | 22.58 ± 2.25 d | 251.58 ± 10.24 gh | 6.49 ± 0.63 de | |
2021 | 182.5 ± 24.2 abc | 6.10 ± 0.69 ab | 77 ± 4 ab | 29.83 ± 0.94 abc | 26.41 ± 3.09 ab | 386.35 ± 29.42 bcd | 20.71 ± 1.04 abcd | |
T10 | 2019 | 149.9 ± 18.6 abcd | 5.71 ± 0.41 abcd | 62 ± 3 a | 12.70 ± 1.36 h | 15.44 ± 0.96 g | 223.24 ± 10.64 f | 2.10 ± 0.19 g |
2020 | 161.9 ± 21.0 cd | 6.96 ± 0.78 b | 72 ± 2 abc | 13.96 ± 1.80 efg | 15.45 ± 1.64 e | 186.97 ± 15.60 ij | 3.26 ± 0.35 gh | |
2021 | 176.5 ± 20.7 abcd | 6.54 ± 0.49 a | 81 ± 6 a | 28.61 ± 1.76 abc | 23.20 ± 2.46 bcd | 407.08 ± 51.68 bc | 21.42 ± 1.95 ab | |
T11 | 2019 | 149.8 ± 7.8 abcd | 6.12 ± 0.62 a | 59 ± 6 ab | 13.29 ± 0.50 gh | 25.80 ± 3.02 f | 289.72 ± 43.97 ef | 2.71 ± 0.43 g |
2020 | 186.9 ± 20.5 abc | 5.96 ± 0.34 cde | 78 ± 6 a | 27.19 ± 3.06 a | 36.76 ± 3.19 a | 453.57 ± 50.83 ab | 12.26 ± 1.16 a | |
2021 | 182.3 ± 9.0 abc | 6.12 ± 0.85 ab | 81 ± 10 a | 29.85 ± 3.20 abc | 25.21 ± 1.85 abc | 447.84 ± 43.60 ab | 19.55 ± 2.87 abcde | |
T12 | 2019 | 149.4 ± 10.5 abcd | 5.00 ± 0.41 cdef | 56 ± 7 ab | 25.23 ± 3.40 de | 54.04 ± 5.07 c | 456.57 ± 42.23 c | 8.22 ± 0.79 c |
2020 | 169.1 ± 27.9 bcd | 5.05 ± 0.14 def | 67 ± 7 abc | 25.29 ± 4.21 ab | 30.32 ± 1.71 bc | 342.48 ± 48.74 def | 9.65 ± 0.88 bc | |
2021 | 170.3 ± 22.6 abcd | 5.97 ± 0.56 ab | 76 ± 8 ab | 27.04 ± 3.05 bcd | 22.20 ± 3.03 cde | 369.72 ± 21.61 cde | 18.77 ± 0.81 bcde | |
T13 | 2019 | 126.6 ± 3.6 d | 4.90 ± 0.23 defg | 51 ± 3 b | 11.42 ± 1.33 h | 19.03 ± 0.41 fg | 236.65 ± 35.58 f | 2.35 ± 0.18 g |
2020 | 201.6 ± 1.7 ab | 4.94 ± 0.75 ef | 68 ± 11 abc | 19.40 ± 2.94 cd | 26.93 ± 3.24 c | 350.39 ± 33.20 de | 10.89 ± 1.41 ab | |
2021 | 175.2 ± 20.5 abcd | 5.22 ± 0.48 b | 66 ± 6 b | 21.94 ± 2.02 e | 17.21 ± 2.03 g | 287.68 ± 17.83 g | 18.18 ± 2.15 bcdef | |
T14 | 2019 | 128.9 ± 4.8 cd | 4.12 ± 0.52 fg | 54 ± 2 ab | 22.48 ± 3.16 e | 36.06 ± 4.56 e | 348.04 ± 32.39 de | 4.91 ± 0.30 f |
2020 | 185.2 ± 14.8 abc | 5.53 ± 0.66 cdef | 63 ± 5 bc | 17.52 ± 1.26 de | 10.04 ± 1.15 f | 133.52 ± 14.90 j | 1.79 ± 0.15 i | |
2021 | 146 ± 16.1 d | 6.27 ± 0.69 ab | 69 ± 2 ab | 31.50 ± 4.55 ab | 24.45 ± 2.29 bc | 421.91 ± 28.79 bc | 22.01 ± 2.02 a | |
T15 | 2019 | 149.4 ± 14.2 abcd | 5.07 ± 0.44 bcde | 62 ± 5 a | 34.74 ± 3.25 a | 81.68 ± 5.21 a | 628.15 ± 78.85 a | 12.73 ± 1.30 a |
2020 | 125.6 ± 13.8 e | 8.28 ± 0.21 a | 65 ± 7 abc | 13.63 ± 1.13 fg | 14.61 ± 0.95 e | 183.74 ± 14.08 ij | 4.56 ± 0.45 fg | |
2021 | 162.0 ± 13.8 abcd | 6.18 ± 0.04 ab | 75 ± 9 ab | 25.08 ± 1.41 cde | 17.76 ± 1.04 fg | 310.56 ± 27.31 efg | 18.76 ± 0.59 bcde | |
T16 | 2019 | 133.7 ± 12.0 bcd | 5.79 ± 0.69 abc | 59 ± 5 ab | 11.88 ± 1.10 h | 24.80 ± 2.37 f | 293.20 ± 29.64 ef | 2.16 ± 0.19 g |
2020 | 191.3 ± 7.5 abc | 5.23 ± 0.62 def | 72 ± 4 abc | 26.54 ± 0.52 a | 29.47 ± 0.45 bc | 466.62 ± 44.12 a | 10.12 ± 1.19 bc | |
2021 | 159.5 ± 23.8 bcd | 5.85 ± 0.72 ab | 68 ± 10 ab | 28.88 ± 2.59 abc | 19.95 ± 0.58 defg | 377.95 ± 35.56cd | 20.88 ± 0.15 abc |
品质指标 Quality index | 最小值 Minimum | 最大值 Maximum | ||||
---|---|---|---|---|---|---|
2019 | 2020 | 2021 | 2019 | 2020 | 2021 | |
可溶性固形物 Total soluble solid | T3 | T2 | T13 | T6 | T11 | T9 |
百粒质量 100 grain weight | T13 | T15 | T14 | T8 | T7 | T8 |
皮果比 Peel-to-fruit ratio | T16 | T5 | T7 | T12 | T15 | T14 |
籽果比 Seed-to-fruit ratio | T14 | T8 | T3 | T11 | T15 | T10 |
籽粒数Number of seeds | T7 | T8 | T13 | T1,T5,T 10,T 15 | T11 | T10,T 11 |
皮总酚 Total peel phenols | T12 | T8 | T14 | T8 | T12 | T4 |
籽总酚 Total seed phenols | T5 | T2 | T13 | T15 | T11 | T3 |
皮花色苷 Peel anthocyanins | T12 | T2 | T2 | T16 | T11 | T4 |
皮总黄酮 Total peel flavonoids | T12 | T2 | T2 | T8 | T6 | T4 |
籽总黄酮 Total seed flavonoids | T10 | T14 | T13 | T15 | T11 | T3 |
皮黄烷醇 Peel flavanols | T12 | T2 | T14 | T2 | T12 | T4 |
籽黄烷醇 Seed flavanols | T5 | T14 | T13 | T3 | T16 | T3 |
皮单宁 Peel tannins | T12 | T9 | T5 | T2 | T12 | T4 |
籽单宁 Seed tannins | T2 | T1 | T4 | T15 | T11 | T3 |
表4 不同年份各果实品质性状极值所在的处理
Table 4 Treatment of the extreme value of each fruit quality trait in different years
品质指标 Quality index | 最小值 Minimum | 最大值 Maximum | ||||
---|---|---|---|---|---|---|
2019 | 2020 | 2021 | 2019 | 2020 | 2021 | |
可溶性固形物 Total soluble solid | T3 | T2 | T13 | T6 | T11 | T9 |
百粒质量 100 grain weight | T13 | T15 | T14 | T8 | T7 | T8 |
皮果比 Peel-to-fruit ratio | T16 | T5 | T7 | T12 | T15 | T14 |
籽果比 Seed-to-fruit ratio | T14 | T8 | T3 | T11 | T15 | T10 |
籽粒数Number of seeds | T7 | T8 | T13 | T1,T5,T 10,T 15 | T11 | T10,T 11 |
皮总酚 Total peel phenols | T12 | T8 | T14 | T8 | T12 | T4 |
籽总酚 Total seed phenols | T5 | T2 | T13 | T15 | T11 | T3 |
皮花色苷 Peel anthocyanins | T12 | T2 | T2 | T16 | T11 | T4 |
皮总黄酮 Total peel flavonoids | T12 | T2 | T2 | T8 | T6 | T4 |
籽总黄酮 Total seed flavonoids | T10 | T14 | T13 | T15 | T11 | T3 |
皮黄烷醇 Peel flavanols | T12 | T2 | T14 | T2 | T12 | T4 |
籽黄烷醇 Seed flavanols | T5 | T14 | T13 | T3 | T16 | T3 |
皮单宁 Peel tannins | T12 | T9 | T5 | T2 | T12 | T4 |
籽单宁 Seed tannins | T2 | T1 | T4 | T15 | T11 | T3 |
品质指标 | 主成分 Principal component | ||
---|---|---|---|
Quality index | PC1 | PC2 | PC3 |
可溶性固形物 Total soluble solid | 0.540 | -0.059 | 0.746 |
百粒质量100 grain weight | 0.114 | 0.418 | -0.519 |
皮果比 Peel-to-fruit ratio | -0.675 | 0.170 | 0.357 |
籽果比 Seed-to-fruit ratio | 0.154 | -0.381 | 0.886 |
籽粒数 Number of seeds | 0.312 | -0.194 | 0.803 |
皮总酚 Total peel phenols | 0.791 | -0.531 | -0.161 |
籽总酚 Total seed phenols | 0.364 | 0.843 | 0.209 |
皮花色苷 Peel anthocyanins | 0.818 | -0.050 | 0.140 |
皮总黄酮 Total peel flavonoids | 0.914 | -0.186 | -0.177 |
籽总黄酮 Total seed flavonoids | 0.373 | 0.857 | -0.005 |
皮黄烷醇 Peel flavanols | 0.808 | -0.397 | -0.291 |
籽黄烷醇 Seed flavanols | 0.597 | 0.751 | 0.006 |
皮单宁 Peel tannins | 0.568 | -0.503 | -0.394 |
籽单宁 Seed tannins | 0.526 | 0.722 | 0.127 |
特征值 Eigenvalue | 4.893 | 3.651 | 2.759 |
贡献率/% Contribution rate | 34.947 | 26.076 | 19.710 |
累计贡献率/% Cumulative contribution rate | 34.947 | 61.023 | 80.733 |
表5 提取的主成分特征值、贡献率及累计贡献率
Table 5 Eigenvalue,contribution rate and cumulative contribution rate of extracted principal component
品质指标 | 主成分 Principal component | ||
---|---|---|---|
Quality index | PC1 | PC2 | PC3 |
可溶性固形物 Total soluble solid | 0.540 | -0.059 | 0.746 |
百粒质量100 grain weight | 0.114 | 0.418 | -0.519 |
皮果比 Peel-to-fruit ratio | -0.675 | 0.170 | 0.357 |
籽果比 Seed-to-fruit ratio | 0.154 | -0.381 | 0.886 |
籽粒数 Number of seeds | 0.312 | -0.194 | 0.803 |
皮总酚 Total peel phenols | 0.791 | -0.531 | -0.161 |
籽总酚 Total seed phenols | 0.364 | 0.843 | 0.209 |
皮花色苷 Peel anthocyanins | 0.818 | -0.050 | 0.140 |
皮总黄酮 Total peel flavonoids | 0.914 | -0.186 | -0.177 |
籽总黄酮 Total seed flavonoids | 0.373 | 0.857 | -0.005 |
皮黄烷醇 Peel flavanols | 0.808 | -0.397 | -0.291 |
籽黄烷醇 Seed flavanols | 0.597 | 0.751 | 0.006 |
皮单宁 Peel tannins | 0.568 | -0.503 | -0.394 |
籽单宁 Seed tannins | 0.526 | 0.722 | 0.127 |
特征值 Eigenvalue | 4.893 | 3.651 | 2.759 |
贡献率/% Contribution rate | 34.947 | 26.076 | 19.710 |
累计贡献率/% Cumulative contribution rate | 34.947 | 61.023 | 80.733 |
处理 Treatment | 主成分得分 Principal component score | 综合评分 Comprehensive score | 得分排序 Score ranking | ||
---|---|---|---|---|---|
Y1(0.433 × PC1) | Y2(0.323 × PC2) | Y3(0.244 × PC3) | Y = Y1 + Y2 + Y3 | ||
T1 | -1.185 | -0.518 | 0.185 | -1.518 | 15 |
T2 | -1.500 | -0.576 | -0.444 | -2.520 | 16 |
T3 | 0.158 | 1.160 | -0.479 | 0.839 | 5 |
T4 | 0.525 | -1.087 | -0.013 | -0.575 | 11 |
T5 | 0.134 | -0.081 | 0.222 | 0.275 | 7 |
T6 | 0.902 | 0.345 | 0.228 | 1.475 | 2 |
T7 | 0.121 | 0.288 | -0.461 | -0.052 | 10 |
T8 | -0.207 | 0.734 | -0.447 | 0.080 | 9 |
T9 | 0.019 | 0.725 | 0.467 | 1.211 | 3 |
T10 | -0.901 | -0.350 | 0.616 | -0.635 | 12 |
T11 | 1.467 | 0.042 | 0.333 | 1.842 | 1 |
T12 | -0.011 | 0.463 | 0.067 | 0.519 | 6 |
T13 | 0.054 | -0.706 | -0.750 | -1.402 | 14 |
T14 | -1.465 | 0.289 | 0.189 | -0.987 | 13 |
T15 | -0.061 | -0.111 | 0.407 | 0.235 | 8 |
T16 | 1.948 | -0.618 | -0.121 | 1.209 | 4 |
表6 不同施肥处理果实各项指标的主成分得分
Table 6 Principal component scores of fruits under different fertilization treatments
处理 Treatment | 主成分得分 Principal component score | 综合评分 Comprehensive score | 得分排序 Score ranking | ||
---|---|---|---|---|---|
Y1(0.433 × PC1) | Y2(0.323 × PC2) | Y3(0.244 × PC3) | Y = Y1 + Y2 + Y3 | ||
T1 | -1.185 | -0.518 | 0.185 | -1.518 | 15 |
T2 | -1.500 | -0.576 | -0.444 | -2.520 | 16 |
T3 | 0.158 | 1.160 | -0.479 | 0.839 | 5 |
T4 | 0.525 | -1.087 | -0.013 | -0.575 | 11 |
T5 | 0.134 | -0.081 | 0.222 | 0.275 | 7 |
T6 | 0.902 | 0.345 | 0.228 | 1.475 | 2 |
T7 | 0.121 | 0.288 | -0.461 | -0.052 | 10 |
T8 | -0.207 | 0.734 | -0.447 | 0.080 | 9 |
T9 | 0.019 | 0.725 | 0.467 | 1.211 | 3 |
T10 | -0.901 | -0.350 | 0.616 | -0.635 | 12 |
T11 | 1.467 | 0.042 | 0.333 | 1.842 | 1 |
T12 | -0.011 | 0.463 | 0.067 | 0.519 | 6 |
T13 | 0.054 | -0.706 | -0.750 | -1.402 | 14 |
T14 | -1.465 | 0.289 | 0.189 | -0.987 | 13 |
T15 | -0.061 | -0.111 | 0.407 | 0.235 | 8 |
T16 | 1.948 | -0.618 | -0.121 | 1.209 | 4 |
生育期 Stage | 器官 Organ | mg · g-1 | |||||
---|---|---|---|---|---|---|---|
N | P | K | Ca | Mg | |||
盛花期 Full-bloom stage | 花序 Inflorescence | 26.61 ± 2.46 b | 7.54 ± 1.74 a | 27.35 ± 4.30 b | 17.46 ± 5.90 b | 4.31 ± 2.06 b | |
叶片Leaf | 32.63 ± 5.78 a | 6.47 ± 1.62 ab | 10.94 ± 0.82 cd | 28.17 ± 5.57 b | 3.72 ± 0.82 b | ||
叶柄Petiole | 14.55 ± 3.09 c | 7.05 ± 0.55 ab | 25.83 ± 6.84 bc | 28.02 ± 8.06 b | 6.82 ± 4.36 ab | ||
转色期 Veraison stage | 果实Fruit | 10.52 ± 1.21 cd | 4.97 ± 1.87 ab | 18.74 ± 1.18 bcd | 15.51 ± 6.43 b | 2.85 ± 1.22 b | |
叶片Leaf | 26.60 ± 4.67 b | 5.76 ± 2.25 ab | 18.34 ± 15.44 bcd | 51.91 ± 17.64 a | 6.60 ± 5.61 ab | ||
叶柄Petiole | 9.19 ± 1.75 cd | 6.50 ± 3.32 ab | 42.08 ± 15.54 a | 53.13 ± 6.26 a | 11.54 ± 1.50 a | ||
成熟期 Maturation stage | 果实Fruit | 7.61 ± 0.58 d | 3.17 ± 1.36 b | 15.46 ± 9.10 bcd | 12.48 ± 16.91 b | 3.59 ± 5.17 b | |
叶片Leaf | 24.30 ± 2.61 b | 4.94 ± 2.29 ab | 7.95 ± 0.94 d | 53.03 ± 19.07 a | 4.86 ± 2.44 b | ||
叶柄Petiole | 8.21 ± 0.93 d | 5.50 ± 2.24 ab | 16.24 ± 3.03 bcd | 54.58 ± 17.05 a | 12.10 ± 2.21 a | ||
生育期 Stage | 器官 Organ | mg · kg-1 | |||||
Fe | Mn | Zn | Cu | B | Mo | ||
盛花期 Full-bloom stage | 花序 Inflorescence | 194.76 ± 50.51 bcd | 80.60 ± 25.53 e | 41.59 ± 8.98 bc | 16.84 ± 3.73 c | 28.43 ± 6.77 a | 0.70 ± 0.18 bc |
叶片Leaf | 277.48 ± 37.07 ab | 233.82 ± 5.54 cd | 37.58 ± 12.68 bcd | 11.18 ± 5.09 c | 27.32 ± 5.77 ab | 1.21 ± 0.32 ab | |
叶柄Petiole | 84.14 ± 28.37 e | 160.72 ± 36.21 de | 35.46 ± 21.91 cd | 9.43 ± 1.82 c | 23.12 ± 4.74 abc | 0.54 ± 0.33 c | |
转色期 Veraison stage | 果实Fruit | 91.78 ± 10.27 e | 34.98 ± 9.12 e | 15.30 ± 1.94 de | 41.95 ± 18.44 c | 22.89 ± 2.95 abc | 0.34 ± 0.13 c |
叶片Leaf | 305.05 ± 106.78 a | 445.99 ± 140.82 b | 59.73 ± 13.29 ab | 1 006.81 ± 524.08 a | 20.53 ± 7.45 abc | 1.60 ± 0.21 a | |
叶柄Petiole | 108.37 ± 47.58 de | 459.48 ± 119.71 b | 65.01 ± 16.70 a | 95.80 ± 40.21 c | 18.36 ± 2.63 b | 0.51 ± 0.20 c | |
成熟期 Maturation stage | 果实Fruit | 129.36 ± 81.56 cde | 20.11 ± 5.83 e | 11.81 ± 0.57 e | 21.40 ± 7.17 c | 18.56 ± 5.15 abc | 0.27 ± 0.23 c |
叶片Leaf | 221.86 ± 42.70 abc | 359.41 ± 33.28 bc | 42.39 ± 2.39 bc | 504.93 ± 370.09 b | 13.76 ± 3.85 c | 1.44 ± 0.78 a | |
叶柄Petiole | 77.27 ± 3.67 e | 682.62 ± 139.45 a | 80.62 ± 14.29 a | 64.00 ± 52.27 c | 18.93 ± 4.57 abc | 0.67 ± 0.20 bc |
表7 2019—2021年最佳处理(T11)各生育期植株矿质元素平均含量
Table 7 Mean content of mineral elements in plants at different growth stages under optimal treatment(T11)from 2019 to 2021
生育期 Stage | 器官 Organ | mg · g-1 | |||||
---|---|---|---|---|---|---|---|
N | P | K | Ca | Mg | |||
盛花期 Full-bloom stage | 花序 Inflorescence | 26.61 ± 2.46 b | 7.54 ± 1.74 a | 27.35 ± 4.30 b | 17.46 ± 5.90 b | 4.31 ± 2.06 b | |
叶片Leaf | 32.63 ± 5.78 a | 6.47 ± 1.62 ab | 10.94 ± 0.82 cd | 28.17 ± 5.57 b | 3.72 ± 0.82 b | ||
叶柄Petiole | 14.55 ± 3.09 c | 7.05 ± 0.55 ab | 25.83 ± 6.84 bc | 28.02 ± 8.06 b | 6.82 ± 4.36 ab | ||
转色期 Veraison stage | 果实Fruit | 10.52 ± 1.21 cd | 4.97 ± 1.87 ab | 18.74 ± 1.18 bcd | 15.51 ± 6.43 b | 2.85 ± 1.22 b | |
叶片Leaf | 26.60 ± 4.67 b | 5.76 ± 2.25 ab | 18.34 ± 15.44 bcd | 51.91 ± 17.64 a | 6.60 ± 5.61 ab | ||
叶柄Petiole | 9.19 ± 1.75 cd | 6.50 ± 3.32 ab | 42.08 ± 15.54 a | 53.13 ± 6.26 a | 11.54 ± 1.50 a | ||
成熟期 Maturation stage | 果实Fruit | 7.61 ± 0.58 d | 3.17 ± 1.36 b | 15.46 ± 9.10 bcd | 12.48 ± 16.91 b | 3.59 ± 5.17 b | |
叶片Leaf | 24.30 ± 2.61 b | 4.94 ± 2.29 ab | 7.95 ± 0.94 d | 53.03 ± 19.07 a | 4.86 ± 2.44 b | ||
叶柄Petiole | 8.21 ± 0.93 d | 5.50 ± 2.24 ab | 16.24 ± 3.03 bcd | 54.58 ± 17.05 a | 12.10 ± 2.21 a | ||
生育期 Stage | 器官 Organ | mg · kg-1 | |||||
Fe | Mn | Zn | Cu | B | Mo | ||
盛花期 Full-bloom stage | 花序 Inflorescence | 194.76 ± 50.51 bcd | 80.60 ± 25.53 e | 41.59 ± 8.98 bc | 16.84 ± 3.73 c | 28.43 ± 6.77 a | 0.70 ± 0.18 bc |
叶片Leaf | 277.48 ± 37.07 ab | 233.82 ± 5.54 cd | 37.58 ± 12.68 bcd | 11.18 ± 5.09 c | 27.32 ± 5.77 ab | 1.21 ± 0.32 ab | |
叶柄Petiole | 84.14 ± 28.37 e | 160.72 ± 36.21 de | 35.46 ± 21.91 cd | 9.43 ± 1.82 c | 23.12 ± 4.74 abc | 0.54 ± 0.33 c | |
转色期 Veraison stage | 果实Fruit | 91.78 ± 10.27 e | 34.98 ± 9.12 e | 15.30 ± 1.94 de | 41.95 ± 18.44 c | 22.89 ± 2.95 abc | 0.34 ± 0.13 c |
叶片Leaf | 305.05 ± 106.78 a | 445.99 ± 140.82 b | 59.73 ± 13.29 ab | 1 006.81 ± 524.08 a | 20.53 ± 7.45 abc | 1.60 ± 0.21 a | |
叶柄Petiole | 108.37 ± 47.58 de | 459.48 ± 119.71 b | 65.01 ± 16.70 a | 95.80 ± 40.21 c | 18.36 ± 2.63 b | 0.51 ± 0.20 c | |
成熟期 Maturation stage | 果实Fruit | 129.36 ± 81.56 cde | 20.11 ± 5.83 e | 11.81 ± 0.57 e | 21.40 ± 7.17 c | 18.56 ± 5.15 abc | 0.27 ± 0.23 c |
叶片Leaf | 221.86 ± 42.70 abc | 359.41 ± 33.28 bc | 42.39 ± 2.39 bc | 504.93 ± 370.09 b | 13.76 ± 3.85 c | 1.44 ± 0.78 a | |
叶柄Petiole | 77.27 ± 3.67 e | 682.62 ± 139.45 a | 80.62 ± 14.29 a | 64.00 ± 52.27 c | 18.93 ± 4.57 abc | 0.67 ± 0.20 bc |
年份 Year | 生育期 Stage | N/(mg · g-1) | P/(mg · g-1) | K/(mg · g-1) | Ca/(mg · g-1) | Mg/(mg · g-1) |
---|---|---|---|---|---|---|
2019 | 萌芽期Germination stage | 0.0995 ± 0.0083 c | 0.7207 ± 0.0033 b | 0.1111 ± 0.0107 b | 0.6508 ± 0.0014 b | 0.0307 ± 0.0008 d |
初花期Initial flowering stage | 0.1099 ± 0.0087c | 0.7652 ± 0.0315 b | 0.1125 ± 0.0102 b | 0.7118 ± 0.0125 a | 0.0716 ± 0.0001 a | |
末花期End bloom stage | 0.1362 ± 0.0101 b | 1.0121 ± 0.0945 a | 0.1728 ± 0.0000 a | 0.5684 ± 0.0044 c | 0.0314 ± 0.0025 d | |
转色期Veraison stage | 0.1786 ± 0.0015 a | 1.1401 ± 0.0626 a | 0.1753 ± 0.0057 a | 0.6024 ± 0.0381 c | 0.0474 ± 0.0044 c | |
成熟期Maturation stage | 0.0659 ± 0.0010 d | 1.0856 ± 0.0971 a | 0.1260 ± 0.0108 b | 0.5222 ± 0.0210 d | 0.0567 ± 0.0045 b | |
2020 | 萌芽期Germination stage | 0.0826 ± 0.0053 c | 0.0471 ± 0.0010 bc | 0.0497 ± 0.0045 d | 7.4751 ± 0.2836 bc | 0.3311 ± 0.0098 c |
初花期Initial flowering stage | 0.0815 ± 0.0004 c | 0.0279 ± 0.0026 d | 0.1075 ± 0.0062 c | 8.2173 ± 0.6376 ab | 0.3646 ± 0.0320 c | |
末花期End bloom stage | 0.1078 ± 0.0002 a | 0.0453 ± 0.0005 c | 0.1020 ± 0.0031 c | 7.2338 ± 0.2683 c | 0.7397 ± 0.0120 b | |
转色期Veraison stage | 0.0950 ± 0.0089 b | 0.0533 ± 0.0020 b | 0.1494 ± 0.0043 a | 8.8712 ± 0.2700 a | 1.0688 ± 0.0480 a | |
成熟期Maturation stage | 0.0477 ± 0.0003 d | 0.1097 ± 0.0070 a | 0.1173 ± 0.0017 b | 7.6929 ± 0.6675 bc | 0.6979 ± 0.0168 b | |
2021 | 萌芽期Germination stage | 0.1059 ± 0.0036 c | 0.1530 ± 0.0031 c | 0.2127 ± 0.0058 a | 6.4961 ± 0.0290 b | 0.7139 ± 0.0273 b |
初花期Initial flowering stage | 0.1411 ± 0.0070 b | 0.2389 ± 0.0200 a | 0.1531 ± 0.0142 b | 6.4551 ± 0.3399 b | 0.5786 ± 0.0285 c | |
末花期End bloom stage | 0.1594 ± 0.0010 a | 0.2219 ± 0.0209 ab | 0.1399 ± 0.0132 bc | 7.1421 ± 0.0727 a | 0.6909 ± 0.0018 b | |
转色期Veraison stage | 0.1541 ± 0.0094 a | 0.2159 ± 0.0066 ab | 0.1267 ± 0.0126 d | 7.1042 ± 0.3704 a | 0.7596 ± 0.0014 a | |
成熟期Maturation stage | 0.0765 ± 0.0033 d | 0.2026 ± 0.0004 b | 0.1225 ± 0.0088 d | 6.1230 ± 0.0166 b | 0.5207 ± 0.0266 d |
表8 最佳处理T11各生育期土壤矿质元素含量
Table 8 Content of mineral elements in soil at different growth stages under optimal treatment(T11)
年份 Year | 生育期 Stage | N/(mg · g-1) | P/(mg · g-1) | K/(mg · g-1) | Ca/(mg · g-1) | Mg/(mg · g-1) |
---|---|---|---|---|---|---|
2019 | 萌芽期Germination stage | 0.0995 ± 0.0083 c | 0.7207 ± 0.0033 b | 0.1111 ± 0.0107 b | 0.6508 ± 0.0014 b | 0.0307 ± 0.0008 d |
初花期Initial flowering stage | 0.1099 ± 0.0087c | 0.7652 ± 0.0315 b | 0.1125 ± 0.0102 b | 0.7118 ± 0.0125 a | 0.0716 ± 0.0001 a | |
末花期End bloom stage | 0.1362 ± 0.0101 b | 1.0121 ± 0.0945 a | 0.1728 ± 0.0000 a | 0.5684 ± 0.0044 c | 0.0314 ± 0.0025 d | |
转色期Veraison stage | 0.1786 ± 0.0015 a | 1.1401 ± 0.0626 a | 0.1753 ± 0.0057 a | 0.6024 ± 0.0381 c | 0.0474 ± 0.0044 c | |
成熟期Maturation stage | 0.0659 ± 0.0010 d | 1.0856 ± 0.0971 a | 0.1260 ± 0.0108 b | 0.5222 ± 0.0210 d | 0.0567 ± 0.0045 b | |
2020 | 萌芽期Germination stage | 0.0826 ± 0.0053 c | 0.0471 ± 0.0010 bc | 0.0497 ± 0.0045 d | 7.4751 ± 0.2836 bc | 0.3311 ± 0.0098 c |
初花期Initial flowering stage | 0.0815 ± 0.0004 c | 0.0279 ± 0.0026 d | 0.1075 ± 0.0062 c | 8.2173 ± 0.6376 ab | 0.3646 ± 0.0320 c | |
末花期End bloom stage | 0.1078 ± 0.0002 a | 0.0453 ± 0.0005 c | 0.1020 ± 0.0031 c | 7.2338 ± 0.2683 c | 0.7397 ± 0.0120 b | |
转色期Veraison stage | 0.0950 ± 0.0089 b | 0.0533 ± 0.0020 b | 0.1494 ± 0.0043 a | 8.8712 ± 0.2700 a | 1.0688 ± 0.0480 a | |
成熟期Maturation stage | 0.0477 ± 0.0003 d | 0.1097 ± 0.0070 a | 0.1173 ± 0.0017 b | 7.6929 ± 0.6675 bc | 0.6979 ± 0.0168 b | |
2021 | 萌芽期Germination stage | 0.1059 ± 0.0036 c | 0.1530 ± 0.0031 c | 0.2127 ± 0.0058 a | 6.4961 ± 0.0290 b | 0.7139 ± 0.0273 b |
初花期Initial flowering stage | 0.1411 ± 0.0070 b | 0.2389 ± 0.0200 a | 0.1531 ± 0.0142 b | 6.4551 ± 0.3399 b | 0.5786 ± 0.0285 c | |
末花期End bloom stage | 0.1594 ± 0.0010 a | 0.2219 ± 0.0209 ab | 0.1399 ± 0.0132 bc | 7.1421 ± 0.0727 a | 0.6909 ± 0.0018 b | |
转色期Veraison stage | 0.1541 ± 0.0094 a | 0.2159 ± 0.0066 ab | 0.1267 ± 0.0126 d | 7.1042 ± 0.3704 a | 0.7596 ± 0.0014 a | |
成熟期Maturation stage | 0.0765 ± 0.0033 d | 0.2026 ± 0.0004 b | 0.1225 ± 0.0088 d | 6.1230 ± 0.0166 b | 0.5207 ± 0.0266 d |
图1 矿质元素与果实品质叠加热图的Mantel Test分析 X1 ~ X14分别代表可溶性固形物、百粒质量、皮果比、籽果比、籽粒数、皮总酚、籽总酚、皮花色苷、皮总黄酮、籽总黄酮、皮黄烷醇、籽黄烷醇、皮单宁和籽单宁;p:植株元素;s:土壤元素。Mantel Test’s r代表品质矩阵和元素矩阵相关性,Mantel Test’s P代表品质矩阵和元素矩阵相关性的显著性,Pearson’s r是指各果实品质之间的相关性。
Fig. 1 Mantel Test analysis of superimposed heat map of mineral elements and fruit qualities X1-X14 represents total soluble solid,100 grain weight,peel-to-fruit ratio,seed-to-fruit ratio,number of seeds,total peel phenols,total seed phenols,peel anthocyanins,total peel flavones,total seed flavones,peel flavones,seed flavanols,peel tannins and seed tannins,respectively. The p and s_ represent plant element and soil element,respectively. Mantel Test’s r represents the correlation between quality matrix and element matrix,Mantel Test’s P represents the significance of the correlation between quality matrix and element matrix,and Pearson’s r refers to the correlation between various fruit qualities.
组织部位 | 元素 | 盛花期 | 转色期 | 成熟期 |
---|---|---|---|---|
Tissue | Element | Full-bloom stage | Veraison stage | Maturation stage |
花序/果实 Inflorescence/fruit | N | 0.175 | -0.401** | -0.190 |
P | 0.767** | 0.527** | 0.361* | |
K | 0.285* | -0.305* | -0.295* | |
Ca | 0.421** | 0.291* | 0.379** | |
Mg | 0.431** | 0.525** | 0.500** | |
Fe | 0.442** | -0.048 | 0.254 | |
Mn | 0.370** | 0.317* | 0.087 | |
Zn | 0.590** | 0.190 | -0.140 | |
Cu | 0.669** | 0.477** | 0.678** | |
B | 0.786** | 0.374** | 0.512** | |
Mo | -0.336* | 0.511** | 0.642** | |
叶片 Leaf | N | -0.616** | -0.530** | -0.405** |
P | 0.583** | 0.235 | 0.296* | |
K | 0.413** | 0.223 | 0.315* | |
Ca | 0.333* | 0.566** | 0.278 | |
Mg | 0.460** | 0.530** | 0.483** | |
Fe | 0.267 | 0.546** | 0.297* | |
Mn | 0.261 | 0.787** | 0.369** | |
Zn | 0.230 | 0.055 | -0.017 | |
Cu | 0.562** | 0.667** | 0.522** | |
B | 0.619** | 0.617** | 0.319* | |
Mo | -0.653** | -0.175 | -0.445** | |
叶柄 Petiole | N | 0.450** | -0.698** | -0.396** |
P | 0.368* | 0.559** | 0.638** | |
K | -0.295* | -0.055 | -0.214 | |
Ca | 0.205 | 0.510** | 0.562** | |
Mg | 0.370** | 0.542** | 0.626** | |
Fe | -0.015 | -0.187 | 0.059 | |
Mn | -0.186 | -0.207 | 0.390** | |
Zn | -0.030 | -0.638** | 0.326* | |
Cu | 0.198 | 0.240 | 0.575** | |
B | 0.157 | -0.318* | 0.471** | |
Mo | -0.640** | -0.520** | -0.462** |
表9 植株矿质元素含量与‘梅乐’葡萄果皮花色苷含量的相关性
Table 9 Correlation coefficients between plant mineral elements and peel anthocyanins
组织部位 | 元素 | 盛花期 | 转色期 | 成熟期 |
---|---|---|---|---|
Tissue | Element | Full-bloom stage | Veraison stage | Maturation stage |
花序/果实 Inflorescence/fruit | N | 0.175 | -0.401** | -0.190 |
P | 0.767** | 0.527** | 0.361* | |
K | 0.285* | -0.305* | -0.295* | |
Ca | 0.421** | 0.291* | 0.379** | |
Mg | 0.431** | 0.525** | 0.500** | |
Fe | 0.442** | -0.048 | 0.254 | |
Mn | 0.370** | 0.317* | 0.087 | |
Zn | 0.590** | 0.190 | -0.140 | |
Cu | 0.669** | 0.477** | 0.678** | |
B | 0.786** | 0.374** | 0.512** | |
Mo | -0.336* | 0.511** | 0.642** | |
叶片 Leaf | N | -0.616** | -0.530** | -0.405** |
P | 0.583** | 0.235 | 0.296* | |
K | 0.413** | 0.223 | 0.315* | |
Ca | 0.333* | 0.566** | 0.278 | |
Mg | 0.460** | 0.530** | 0.483** | |
Fe | 0.267 | 0.546** | 0.297* | |
Mn | 0.261 | 0.787** | 0.369** | |
Zn | 0.230 | 0.055 | -0.017 | |
Cu | 0.562** | 0.667** | 0.522** | |
B | 0.619** | 0.617** | 0.319* | |
Mo | -0.653** | -0.175 | -0.445** | |
叶柄 Petiole | N | 0.450** | -0.698** | -0.396** |
P | 0.368* | 0.559** | 0.638** | |
K | -0.295* | -0.055 | -0.214 | |
Ca | 0.205 | 0.510** | 0.562** | |
Mg | 0.370** | 0.542** | 0.626** | |
Fe | -0.015 | -0.187 | 0.059 | |
Mn | -0.186 | -0.207 | 0.390** | |
Zn | -0.030 | -0.638** | 0.326* | |
Cu | 0.198 | 0.240 | 0.575** | |
B | 0.157 | -0.318* | 0.471** | |
Mo | -0.640** | -0.520** | -0.462** |
元素 | 萌芽期 | 初花期 | 末花期 | 转色期 | 成熟期 |
---|---|---|---|---|---|
Element | Germination stage | Initial flowering stage | End bloom stage | Veraison stage | Maturation stage |
N | -0.383** | -0.362* | -0.351* | 0.248 | 0.126 |
P | 0.465** | 0.460** | 0.525** | 0.549** | 0.553** |
K | -0.598** | -0.521** | -0.365* | 0.178 | -0.145 |
Ca | -0.532** | -0.547** | -0.563** | -0.503** | -0.632** |
Mg | -0.784** | -0.744** | -0.536** | -0.543** | -0.607** |
表10 土壤矿质元素含量与‘梅乐’葡萄果皮花色苷含量的相关性
Table 10 Correlation coefficients between soil mineral elements and peel anthocyanins
元素 | 萌芽期 | 初花期 | 末花期 | 转色期 | 成熟期 |
---|---|---|---|---|---|
Element | Germination stage | Initial flowering stage | End bloom stage | Veraison stage | Maturation stage |
N | -0.383** | -0.362* | -0.351* | 0.248 | 0.126 |
P | 0.465** | 0.460** | 0.525** | 0.549** | 0.553** |
K | -0.598** | -0.521** | -0.365* | 0.178 | -0.145 |
Ca | -0.532** | -0.547** | -0.563** | -0.503** | -0.632** |
Mg | -0.784** | -0.744** | -0.536** | -0.543** | -0.607** |
图2 ‘梅乐’葡萄植株和土壤大中量元素分析参数与皮花色苷之间的函数关系式 CI:品质指数,表示利用极差法将各处理皮花色苷进行标准化处理后的数据,下同。
Fig. 2 Functional relationship between large and medium element in plant and soil and peel anthocyanins CI:Quality index,represents the data after standardized processing of peel anthocyanins for each treatment using range method. The same below.
类型 Type | 营养诊断因子 Nutritional diagnostic factor | 高质园High quality orchard | 低质园 Low quality orchard | |||||
---|---|---|---|---|---|---|---|---|
最低值/ (mg · g-1) Min | 最高值/ (mg · g-1) Max | CNDr2 | 果园数量 Number of orchards | Ix | CNDr2 | 果园数量 Number of orchards | ||
植株大中量元素 Plant macroel and medium element | 转色期叶柄N Veraison stage-petiole N | 5.38 | 11.00 | 5.14 | 7 | 0.08 | 10.15 | 41 |
盛花期果实P Full-bloom stage-fruit P | 5.45 | 10.88 | -0.08 | |||||
盛花期叶片K Full-bloom stage-leaf K | 6.73 | 39.75 | -0.31 | |||||
转色期叶片Ca Veraison stage-leaf Ca | 35.57 | 68.83 | -0.52 | |||||
成熟期叶柄Mg Maturation stage-petiole Mg | 9.30 | 24.63 | 0.52 | |||||
植株微量元素 Plant trace element | 转色期叶片Fe Veraison stage-leaf Fe | 233.93 × 10-3 | 398.83 × 10-3 | 5.00 | 6 | 0.26 | 11.18 | 42 |
转色期叶片Mn Veraison stage-leaf Mn | 292.55 × 10-3 | 520.30 × 10-3 | -0.61 | |||||
转色期叶柄Zn Veraison stage-petiole Zn | 29.03 × 10-3 | 73.65 × 10-3 | 0.18 | |||||
成熟期果实Cu Maturation stage-fruit Cu | 18.22 × 10-3 | 28.93 × 10-3 | -0.05 | |||||
盛花期果实B Full-bloom stage-fruit B | 20.52 × 10-3 | 35.13 × 10-3 | -0.80 | |||||
盛花期叶片Mo Full-bloom stage-leaf Mo | 0.78 × 10-3 | 1.85 × 10-3 | 0.32 | |||||
土壤大中量元素 Soil macroel and medium element | 萌芽期N Germination stage N | 0.06 | 0.21 | 5.50 | 12 | -0.29 | 6.83 | 36 |
成熟期P Maturation stage P | 0.08 | 1.17 | -0.24 | |||||
萌芽期K Germination stage K | 0.05 | 0.23 | 0.34 | |||||
成熟期Ca Maturation stage Ca | 0.52 | 8.51 | 0.12 | |||||
萌芽期Mg Germination stage Mg | 0.04 | 0.65 | 0.29 |
表11 ‘梅乐’葡萄高质果园和低质果园植株和土壤营养元素含量分布特征
Table 11 Distribution characteristics of plant and soil elements in different orchards
类型 Type | 营养诊断因子 Nutritional diagnostic factor | 高质园High quality orchard | 低质园 Low quality orchard | |||||
---|---|---|---|---|---|---|---|---|
最低值/ (mg · g-1) Min | 最高值/ (mg · g-1) Max | CNDr2 | 果园数量 Number of orchards | Ix | CNDr2 | 果园数量 Number of orchards | ||
植株大中量元素 Plant macroel and medium element | 转色期叶柄N Veraison stage-petiole N | 5.38 | 11.00 | 5.14 | 7 | 0.08 | 10.15 | 41 |
盛花期果实P Full-bloom stage-fruit P | 5.45 | 10.88 | -0.08 | |||||
盛花期叶片K Full-bloom stage-leaf K | 6.73 | 39.75 | -0.31 | |||||
转色期叶片Ca Veraison stage-leaf Ca | 35.57 | 68.83 | -0.52 | |||||
成熟期叶柄Mg Maturation stage-petiole Mg | 9.30 | 24.63 | 0.52 | |||||
植株微量元素 Plant trace element | 转色期叶片Fe Veraison stage-leaf Fe | 233.93 × 10-3 | 398.83 × 10-3 | 5.00 | 6 | 0.26 | 11.18 | 42 |
转色期叶片Mn Veraison stage-leaf Mn | 292.55 × 10-3 | 520.30 × 10-3 | -0.61 | |||||
转色期叶柄Zn Veraison stage-petiole Zn | 29.03 × 10-3 | 73.65 × 10-3 | 0.18 | |||||
成熟期果实Cu Maturation stage-fruit Cu | 18.22 × 10-3 | 28.93 × 10-3 | -0.05 | |||||
盛花期果实B Full-bloom stage-fruit B | 20.52 × 10-3 | 35.13 × 10-3 | -0.80 | |||||
盛花期叶片Mo Full-bloom stage-leaf Mo | 0.78 × 10-3 | 1.85 × 10-3 | 0.32 | |||||
土壤大中量元素 Soil macroel and medium element | 萌芽期N Germination stage N | 0.06 | 0.21 | 5.50 | 12 | -0.29 | 6.83 | 36 |
成熟期P Maturation stage P | 0.08 | 1.17 | -0.24 | |||||
萌芽期K Germination stage K | 0.05 | 0.23 | 0.34 | |||||
成熟期Ca Maturation stage Ca | 0.52 | 8.51 | 0.12 | |||||
萌芽期Mg Germination stage Mg | 0.04 | 0.65 | 0.29 |
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