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园艺学报 ›› 2024, Vol. 51 ›› Issue (11): 2575-2593.doi: 10.16420/j.issn.0513-353x.2023-0563

• 栽培·生理生化 • 上一篇    下一篇

酿酒葡萄‘梅乐’植株和土壤营养水平与果实品质的关联性分析

王小龙1,*, 邵学东2,*, 张正文2, 钟晓敏2, 刘畅1, 王志强1, 王宝亮1, 冀晓昊1, 史祥宾1, 王海波1,**()   

  1. 1 中国农业科学院果树研究所,农业农村部园艺作物种质资源利用重点实验室,辽宁省落叶果树矿质营养与肥料高效利用重点实验室,辽宁兴城 125100
    2 君顶酒庄有限公司,山东蓬莱 265600
  • 收稿日期:2024-06-25 修回日期:2024-08-16 出版日期:2024-12-12 发布日期:2024-11-25
  • 通讯作者:
    ** E-mail:
  • 作者简介:

    共同第一作者

  • 基金资助:
    现代农业产业技术体系建设专项资助(nycytx-30-zp); 中国农业科学院科技创新工程项目(CAAS-ASTIP-2016-RIP-04)

Correlation Analysis of Fruit Quality and the Nutrient Level in Plant and Soil of Wine Grape‘Merlot’

WANG Xiaolong1, SHAO Xuedong2, ZHANG Zhengwen2, ZHONG Xiaomin2, LIU Chang1, WANG Zhiqiang1, WANG Baoliang1, JI Xiaohao1, SHI Xiangbin1, WANG Haibo1,**()   

  1. 1 Key Laboratory of Germplasm Resources Utilization of Horticultural Crops,Ministry of Agriculture and Rural Affairs,Key Laboratory of Mineral Nutrition and Fertilizers Efficient Utilization of Deciduous Fruit Tree,Fruit Research Institute,Chinese Academy of Agricultural Sciences,Xingcheng,Liaoning 125100,China
    2 Junding Winery Co. Ltd.,Penglai,Shandong 265600,China
  • Received:2024-06-25 Revised:2024-08-16 Published:2024-12-12 Online:2024-11-25

摘要:

以山东烟台产区‘梅乐’酿酒葡萄为试材,于2019—2021年结合正交设计L16(45)‘5416’配方施肥方案,利用主成分分析法(PCA)对各施肥处理成熟期果实的百粒质量、可溶性固形物、皮果比、籽果比、籽粒数、果皮和种子(皮/籽)总酚、皮花色苷、皮/籽总黄酮、皮/籽黄烷醇、皮/籽单宁等14个品质指标进行综合评价。PCA结果表明,T11处理(N 124.5 kg · hm-2、P2O5 46.5 kg · hm-2、K2O 0 kg · hm-2、CaO 56.3 kg · hm-2和MgO 69.8 kg · hm-2)对提高‘梅乐’葡萄综合品质的效果较为明显。利用相关性分析法,确定萌芽期0 ~ 40 cm土壤N、K、Mg和成熟期P、Ca含量为花色苷的土壤营养诊断因子,确定转色期叶柄N,盛花期花序P、Zn、B,盛花期叶片K、Mo,转色期叶片Ca、Fe和Mn,成熟期叶柄Mg,成熟期果实Cu含量为花色苷的植株营养诊断因子。利用组分营养诊断法(CND)建立诊断因子与花色苷的函数模型,最终制定高花色苷葡萄园(即高质葡萄园)土壤和植株的营养诊断标准:高质园(土壤元素含量累积方差所对应的花色苷归一化值 > 0.6372,即拐点值)的土壤营养诊断标准为N 0.06 ~ 0.21 mg · g-1、P 0.08 ~ 1.17 mg · g-1、K 0.05 ~ 0.23 mg · g-1、Ca 0.52 ~ 8.51 mg · g-1和Mg 0.04 ~ 0.65 mg · g-1;高质园(植株大中量元素含量累积方差所对应的花色苷归一化值 > 0.8353;微量元素含量累积方差所对应的花色苷归一化值 > 0.8699)的植株营养诊断标准为N 5.38 ~ 11.00 mg · g-1、P 5.45 ~ 10.88 mg · g-1、K 6.73 ~ 39.75 mg · g-1、Ca 35.57 ~ 68.83 mg · g-1、Mg 9.30 ~ 24.63 mg · g-1、Fe 233.93 ~ 398.83 mg · kg-1、Mn 292.55 ~ 520.30 mg · kg-1、Zn 29.03 ~ 73.65 mg · kg-1、Cu 18.22 ~ 28.93 mg · kg-1、B 20.52 ~ 35.13 mg · kg-1和Mo 0.78 ~ 1.85 mg · kg-1;针对‘梅乐’葡萄低质园(植株大中量元素含量累积方差所对应的花色苷归一化值 < 0.8353;土壤营养元素含量累积方差所对应的花色苷归一化值 < 0.6372)的植株和土壤丰缺特点,建议采用少量多次的方式向土壤施用P肥;完善水肥管理制度,提高植株对K和Ca元素的吸收能力;通过叶面喷施适当补充缺乏的微量元素。

关键词: 酿酒葡萄, 土壤, 植株, 营养诊断, 主成分分析, 品质

Abstract:

The aim is to lay a foundation for improving the quality of Merlot fruit and guiding precise fertilization in orchards. Taking Merlot grapewine in Yantai,Shandong Province as the test material,combined with the“5416”formula fertilization program from 2019 to 2021,principal component analysis (PCA)was used to evaluate 14 quality indexes of fruit at maturity stage,including 100 grain mass,soluble solid matter,ratio of skin to fruit,ratio of seed to seed,number of grains,total phenols,anthocyanins in peel,total flavonoids in peel and seed,flavanols in peel and seed,and tannins in peel and seed. PCA results show that T11 treatment(N 124.5 kg · hm-2,P2O5 46.5 kg · hm-2,K2O 0 kg · hm-2,CaO 56.3 kg · hm-2 and MgO 69.8 kg · hm-2)had obvious effect on improving the comprehensive quality of Merlot grapewine. Correlation analysis was used to determine N in germination stage,P in maturation stage,K in maturation stage,Ca in maturation stage and Mg in germination stage distributed in 0-40 cm as soil nutrient diagnostic factors for anthocyanins. Plant nutritional diagnostic factors for anthocyanins were determined:N of petiole at veraison stage,P of inflorescence at full-bloom stage,K of leaf at full-bloom stage,Ca of leaf at veraison stage,Mg of petiole at maturity stage,Fe and Mn of leaf at veraison stage,Cu of fruit at maturity stage,Zn and B of inflorescence at full-bloom and Mo of leaf at full-bloom stage. The function model of diagnostic factors and anthocyanins was established by the method of component nutrition diagnosis(CND),and the diagnostic criteria for soil and plant nutrition of high-anthocyanin vineyards(high-quality vineyards)were finally established:The normalized value of anthocyanins corresponding to cumulative variance of soil element content > 0.6372;The normalized value of anthocyanins corresponding to the cumulative variance of plant medium and large elements content was > 0.8353;The normalized value of anthocyanin corresponding to cumulative variance of trace element content was > 0.8699. The diagnostic criteria for soil nutrition were N 0.06-0.21 mg · g-1,P 0.08-1.17 mg · g-1,K 0.05-0.23 mg · g-1,Ca 0.52-8.51 mg · g-1 and Mg 0.04-0.65 mg · g-1. The diagnostic criteria of plant nutrition were N 5.38-11.00 mg · g-1,P 5.45-10.88 mg · g-1,K 6.73-39.75 mg · g-1,Ca 35.57-68.83 mg · g-1,Mg 9.30-24.63 mg · g-1,Fe 233.93-398.83 mg · kg-1,Mn 292.55-520.30 mg · kg-1,Zn 29.03-73.65 mg · kg-1,Cu 18.22-28.93 mg · kg-1,B 20.52-35.13 mg · kg-1 and Mo 0.78-1.85 mg · kg-1. According to the abundance and deficiency characteristics of plants and soil elements in low quality vineyard(the normalized value of anthocyanins corresponding to the cumulative variance of the content of large and medium elements in the low-quality vineyards of Melot grapewine was < 0.8353;the normalized value of anthocyanin corresponding to the cumulative variance of soil elements was < 0.6372),it is recommended to apply P fertilizer to soil in a small amount and multiple times. Improve the water and fertilizer management system to improve the absorption capacity of K and Ca elements. The deficient trace elements are properly supplemented by foliar spraying.

Key words: wine grape, soil, plant, nutritional diagnosis, principal component analysis, quality