https://www.ahs.ac.cn/images/0513-353X/images/top-banner1.jpg|#|苹果
https://www.ahs.ac.cn/images/0513-353X/images/top-banner2.jpg|#|甘蓝
https://www.ahs.ac.cn/images/0513-353X/images/top-banner3.jpg|#|菊花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner4.jpg|#|灵芝
https://www.ahs.ac.cn/images/0513-353X/images/top-banner5.jpg|#|桃
https://www.ahs.ac.cn/images/0513-353X/images/top-banner6.jpg|#|黄瓜
https://www.ahs.ac.cn/images/0513-353X/images/top-banner7.jpg|#|蝴蝶兰
https://www.ahs.ac.cn/images/0513-353X/images/top-banner8.jpg|#|樱桃
https://www.ahs.ac.cn/images/0513-353X/images/top-banner9.jpg|#|观赏荷花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner10.jpg|#|菊花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner11.jpg|#|月季
https://www.ahs.ac.cn/images/0513-353X/images/top-banner12.jpg|#|菊花

园艺学报 ›› 2023, Vol. 50 ›› Issue (5): 959-971.doi: 10.16420/j.issn.0513-353x.2022-0190

• 遗传育种·种质资源·分子生物学 • 上一篇    下一篇

外源山梨醇影响桃叶片和果实糖代谢的分子机制研究

周平, 郭瑞, 颜少宾, 金光()   

  1. 福建省农业科学院果树研究所,福州 350013
  • 收稿日期:2022-11-03 修回日期:2023-04-27 出版日期:2023-05-25 发布日期:2023-05-31
  • 通讯作者: *(E-mail:jinguang0591@163.com
  • 基金资助:
    福建省省属公益类科研院所基本科研专项(2022R1028009);福建省省属公益类科研院所基本科研专项(2021R10280011);福建省省属公益类科研院所基本科研专项(2020R1028003);福建省省属公益类科研院所基本科研专项(2018R1013-13);福建省省属公益类科研院所基本科研专项(2017R1013-1);财政部和农业农村部:国家现代农业产业技术体系(CARS-30-Z-07);福建省农业科学院科技创新团队项目(CXTD2021009-2)

Molecular Mechanism Study of Exogenous Sorbitol Effects on Sugar Metabolism in Peach Leaves and Fruits

ZHOU Ping, GUO Rui, YAN Shaobin, JIN Guang()   

  1. Fruit Research Institute,Fujian Academy of Agricultural Sciences,Fuzhou 350013,China
  • Received:2022-11-03 Revised:2023-04-27 Published:2023-05-25 Online:2023-05-31

摘要:

为研究桃树(Prunus persica)外源喷施山梨醇(1 mmol · L-1)对其糖代谢的影响,整合代谢组与转录组数据,分析叶片和果肉中蔗糖、果糖、葡萄糖、山梨醇的含量和特定糖代谢、糖转运基因表达的变化,以期明确受影响的代谢通路。测定结果表明,山梨醇处理叶片葡萄糖、果糖、蔗糖含量提升,山梨醇含量变化不明显;处理果肉山梨醇含量下降,其他糖含量变化不明显;处理叶片和果肉蔗糖/果糖、蔗糖/葡萄糖、山梨醇/果糖、山梨醇/葡萄糖和山梨醇/蔗糖比值均显著下降。山梨醇处理后,叶片和果肉糖代谢、糖转运基因表达谱变化不相同,糖代谢酶(PpSDHb、PpPFKa/b/e、PpHXKa、PpSUSb/c、PpINVe)和糖转运子(PpSOTa/i、PpSWEET10/13)基因参与调控叶片和果肉中的蔗糖、果糖、葡萄糖和山梨醇积累。转录组分析证实山梨醇处理造成的差异表达基因富集于糖代谢相关途径(叶片蔗糖、淀粉代谢途径和果肉碳代谢途径)及其他次级代谢途径。这些结果说明外源山梨醇对桃叶片和果实果肉可溶性糖含量和糖组分平衡的影响不同。山梨醇处理造成桃叶片、果实糖代谢酶和糖转运子基因表达变化,影响了糖代谢。

关键词: 桃, 叶片, 果实, 外源山梨醇, 糖代谢, 分子机制

Abstract:

To explore the effects of exogenous sorbitol on leaves and fruits of peach(Prunus persica),the metabolomics and transcriptomics were integrated to investigate changes in sugar(sucrose,glucose,fructose,and sorbitol)contents and expression levels of genes involved in sugar metabolism and transportation as well as relevant metabolic pathways. The results have shown that sucrose,glucose,and fructose contents increased,while there was no difference in sorbitol content in leaves under sorbitol treatment. However,sorbitol content decreased while other sugar contents remained constant in the flesh under sorbitol treatment. Furthermore,content ratios of sucrose/glucose,sucrose/fructose,sorbitol/glucose,sorbitol/fructose,and sorbitol/sucrose were dramatically declined in both leaves and the flesh. Transcriptional patterns of leaves and the flesh under exogenous sorbitol treatment were distinct,in which sugar-related genes including sugar-metabolizing enzymes(PpSDHb,PpPFKa/b/e,PpHXKa,PpSUSb/c and PpINVe)and sugar transporters(PpSOTa/I and PpSWEET10/13),mediated the sucrose,glucose,fructose and sorbitol accumulation in different organs. Transcriptomic analyses confirmed that differentially expressed genes were overrepresented in sugar metabolism-related pathways(including starch and sucrose metabolism in leaves and carbon metabolism in fruits)and other secondary metabolism processes. Our study suggested that exogenous sorbitol influenced soluble sugar contents and sugar composition balance of peach leaves and flesh differently. The leaves- and fruits-specific transcriptional changes of sugar-metabolizing and sugar transporting genes fine-tuned the sugar metabolism.

Key words: peach, leaf, fruit, exogenous sorbitol, sugar metabolism, molecular mechanism

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