番茄中ABC转运蛋白SlABCG23调控茉莉酸信号途径

doi:10.16420/j.issn.0513-353x.2022-0160

摘要/Abstract

摘要：

茉莉酸（jasmonic acid，JA）的运输依赖于转运蛋白的参与。ABC（ATP binding cassette）转运蛋白参与植物体内多种物质的运输。已知在番茄中存在4个与拟南芥中JA转运蛋白具有较高同源性的ABC转运蛋白。对其进行了Motif、蛋白质结构域和跨膜结构分析，并选取其中的SlABCG23进行功能初步鉴定，推测其可能与JA信号途径的调控有关。构建了SlABCG23的过表达载体，通过农杆菌侵染法得到3株过表达番茄植株。通过表型分析发现，SlABCG23过表达植株的花器官增大，花粉萌发受阻，果实内种子发育异常且体内JA含量降低。推测SlABCG23可能通过影响JA的含量参与调控JA信号途径。

关键词: 番茄, 茉莉酸, 转运蛋白, SlABCG23

Abstract:

The transport of jasmonic acid（JA）depends on the participation of transporters. ABC（ATP binding cassette）transporter is involved in the transport of various substances in plants. Four ABC transporters with high homology to the JA transporter in Arabidopsis thaliana are known to exist in tomato. Motif，protein conserved domain and transmembrane structure analysis were performed，finally，SlABCG23 was selected for preliminary function identification. It was speculated that SlABCG23 may be related to the regulation of JA signaling pathway. The overexpression vector of SlABCG23 was constructed，and three overexpression tomato plants were obtained by Agrobacterium infection. Phenotypic analysis showed that SlABCG23 overexpressed plants had enlarged flower organs，blocked pollen germination，abnormal seed development in fruits，following with decreased JA content in vivo. Therefore，it is speculated that SlABCG23 might be involved in regulating JA signaling pathway by affecting JA content.

Key words: tomato, jasmonic acid, transporter protein, SlABCG23

中图分类号:

S641.2

刘玉菡, 陶宁, 王庆国, 李清清. 番茄中ABC转运蛋白SlABCG23调控茉莉酸信号途径[J]. 园艺学报, 2023, 50(3): 559-568.

LIU Yuhan, TAO Ning, WANG Qingguo, LI Qingqing. ABC Transporter SlABCG23 Regulates Jasmonic Acid Signaling Pathway in Tomato[J]. Acta Horticulturae Sinica, 2023, 50(3): 559-568.

图/表 9

表1 本研究中所用引物

Table 1 The primers used in this study

用途 Function	引物名称 Primer name	序列（5′-3′） Sequence
全长扩增 Full length amplification	SlABCG23	F：GAGCTCATGTCTAGTCTTTGTGGAGGC R：GGTACCCCTCCTCTTATTTTTGCTTCCA
qRT-PCR	qSlABCG23	F：GCTTATCGCCGTTCCTCTTATG R：GGCTAGTCCAACTGACCAATATG
qRT-PCR	SlActin	F：TGTCCCTATTTACGAGGGTTATGC R：CAGTTAAATCACGACCAGCAAGAT
过表达植株鉴定Identification of overexpressing lines	OE-SlABCG23	F：GCACAATCCCACTATCCTTCGCA R：AGCAGAGTCGCCAAAGCGTTT

图1 Motif分析

Fig. 1 Motif analysis

图2 SlABCG23蛋白质保守结构域分析

Fig. 2 Analysis of SlABCG23 protein conserved domains

图3 SlABCG23跨膜结构域分析

Fig. 3 Analysis of SlABCG23 transmembrane domain

图4 SlABCG23在番茄不同组织中的表达特异性不同小写字母代表差异性显著（P < 0.05），下同。

Fig. 4 The expression level of SlABCG23 in different tissues Different lowercase letters represent significant differences（P < 0.05），the same below.

图5 SlABCG23转基因阳性番茄植株（OE1、OE6和OE11）PCR鉴定（A）、表达量半定量（B）和定量（C）

Fig. 5 PCR confirmation of SlABCG23 overexpressing lines（OE1，OE6 and OE11）（A），RT-PCR（B）and qRT-PCR（C）

图6 番茄过表达SlABCG23株系（OE1、OE6和OE11）花器官表型（A）和长度（B）以及果实表型（C）

Fig. 6 The phenotype of SlABCG23 overexpressed plants（OE1，OE6 and OE11）flowers（A）,the length of flower（B）and fruits（C）

图7 番茄野生型（WT）和过表达SlABCG23株系（OE1、OE6和OE11）花粉形态及萌发观察

Fig. 7 Pollen morphology and germination of wild type（WT）and SlABCG23 overexpressed lines（OE1，OE6 and OE11）

图8 番茄野生型（WT）和SlABCG2过表达（OE）株系花器官中的JA含量

Fig. 8 The JA content in flower organs of wild type（WT）and SlABCG23 overexpressed tomato plants（OE）

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