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园艺学报 ›› 2021, Vol. 48 ›› Issue (7): 1340-1348.doi: 10.16420/j.issn.0513-353x.2021-0206

• 研究论文 • 上一篇    下一篇

番茄节间长度基因IL10IL11的鉴定和功能验证

刘根忠, 石春美, 于会洋, 王莹, 陈卫芳, 尚乐乐, 张余洋*(), 叶志彪   

  1. 华中农业大学园艺植物生物学教育部重点实验室,武汉 430070
  • 收稿日期:2021-04-27 修回日期:2021-05-28 出版日期:2021-07-25 发布日期:2021-08-10
  • 通讯作者: 张余洋 E-mail:yyzhang@mail.hzau.edu.cn
  • 基金资助:
    国家重点研发计划项目(2016YFD0100204-21);国家重点研发计划项目(2018YFD1000800);国家自然科学基金项目(31991182);国家自然科学基金项目(31972426);武汉市应用基础前沿项目(2019020701011492)

Identification and Functional Verification of Tomato Stem Internode Length Genes IL10 and IL11

LIU Genzhong, SHI Chunmei, YU Huiyang, WANG Ying, CHEN Weifang, SHANG Lele, ZHANG Yuyang*(), YE Zhibiao   

  1. Key Laboratory of Horticultural Plant Biology,Ministry of Education,Huazhong Agricultural University,Wuhan 430070,China
  • Received:2021-04-27 Revised:2021-05-28 Online:2021-07-25 Published:2021-08-10
  • Contact: ZHANG Yuyang E-mail:yyzhang@mail.hzau.edu.cn

摘要:

通过对266份番茄核心种质资源第3穗果实处节间长度进行测定,进行全基因组关联分析,共鉴定7个与节间长度相关的QTL位点。对10和11号染色体中的显著性SNP进行候选基因分析,进一步挖掘控制番茄节间长度的主效基因IL10(Forkhead-associated domain protein)和IL11(Auxin-regulated protein)。以‘Ailsa Craig’番茄为背景材料,通过农杆菌介导的方法进行遗传转化,获得了11株IL10基因沉默、10株IL11基因沉默的株系,其节间长度比野生型短。综上所述,IL10IL11为控制番茄节间长度的关键基因,对番茄节间长度起正向调控作用。

关键词: 番茄, 茎秆, 节间长度, 全基因组关联分析, 候选基因

Abstract:

We measured and analyzed internode length at the third panicle of 266 tomato accessions. Seven QTL related to tomato internode length were identified by genome-wide association study. Candidate genes were analyzed based on the significant SNPs in chromosomes 10 and 11. The IL10(Forkhead- associated domain protein)and IL11(Auxin-regulated protein)related to the internode length were further excavated. Tomato‘Ailsa Craig’was used as the background material. 11 IL10-RNAi lines and 10 IL11-RNAi lines were obtained by Agrobacterium-mediated transformation. The internode length of IL10- and IL11-silencing transgenic lines was significantly shorter than that of the wild type. To sum up,IL10 and IL11 are the key genes that positively regulates tomato internode length.

Key words: tomato, stem, internode length, genome-wide association study, candidate gene

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