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园艺学报 ›› 2021, Vol. 48 ›› Issue (5): 947-959.doi: 10.16420/j.issn.0513-353x.2020-0800

• 研究论文 • 上一篇    下一篇

辣椒PEBP基因家族的全基因组鉴定、比较进化与组织表达分析

牛西强1, 罗潇云1, 康凯程2, 黄先忠1,2,*(), 胡能兵1, 隋益虎1, 艾昊1   

  1. 1安徽科技学院农学院,安徽凤阳 233100
    2石河子大学生命科学学院,植物基因组学实验室,新疆石河子 832003
  • 收稿日期:2020-12-07 修回日期:2021-03-21 出版日期:2021-05-25 发布日期:2021-06-07
  • 通讯作者: 黄先忠 E-mail:huangxz@ahstu.edu.cn
  • 基金资助:
    国家自然科学基金项目(31860393);安徽省科技攻关面上项目(1704g07020111);安徽科技学院学科带头人引进人才启动经费项目(NXYJ202001);安徽省大学生创新创业训练计划项目(S202010879303)

Genome-wide Identification,Comparative Evolution and Expression Analysis of PEBP Gene Family from Capsicum annuum

NIU Xiqaing1, LUO Xiaoyun1, KANG Kaicheng2, HUANG Xianzhong1,2,*(), HU Nengbing1, SUI Yihu1, AI Hao1   

  1. 1College of Agriculture,Anhui Science and Technology University,Fengyang,Anhui 233100,China
    2Plant Genomics Laboratory,College of Life Sciences,Shihezi University,Shihezi,Xinjiang 832003,China
  • Received:2020-12-07 Revised:2021-03-21 Online:2021-05-25 Published:2021-06-07
  • Contact: HUANG Xianzhong E-mail:huangxz@ahstu.edu.cn

摘要:

植物磷脂酰乙醇胺结合蛋白(Phosphatidyl ethanolamine-binding protein,PEBP)分为MFT、FT和TFL1共3个亚家族,其中FT和TFL1亚家族蛋白构成了植物的成花激素—抗成花激素系统,调控开花时间和株形结构。基于辣椒(Capsicum annuum)全基因组数据,对辣椒PEBP基因家族进行鉴定,并对基因和蛋白结构、比较进化、共线性和组织表达特征等进行了分析。结果表明,辣椒基因组包含9个PEBP成员,并且9个基因均含有4个外显子和3个内含子,定位在6条染色体上,其编码蛋白质定位在细胞质和细胞核中。与番茄PEBP同源基因的比较进化分析表明,辣椒的PEBP基因受纯化选择,其中CaFT2CaFT4在进化中较稳定,辣椒和番茄之间存在8对共线性基因。qRT-PCR分析表明CaPEBP基因在所检测的组织中明显差异表达,如CaMFT/CaTFL1-1在果实中高表达,CaFT1在叶片中相对表达量最高,CaFT3在顶端分生组织和花中表达量最高,CaTFL1-4在根中特异表达。

关键词: 辣椒, 成花素, PEBP基因家族, 全基因组鉴定, 开花调控, 基因表达

Abstract:

The plant phosphatidyl ethanolamine-binding protein(PEBP)is divided into three subfamilies:MFT-like,FT-like,and TFL1-like. The FT and TFL1 subfamilies comprise the florigen-antiflorigen system of plants that regulate flowering time and plant architecture. Here,the PEBP gene family was identified by genome-wide identification of the whole genome data of the annual pepper (Capsicum annuum),and the structures of gene and protein,comparative evolution,collinearity and tissue expression profile were analyzed. The results showed that the C. annuum genome contains nine PEBP genes,including one MFT,four FT,and four TFL1 genes. All the nine PEBPgenes contain four exons and three introns,which are located on six chromosomes. Moreover,these nine PEBP proteins are located in the cytoplasm and nucleus. Comparative evolution analysis between C. annuum and Solanum lycopersicum showed that CaPEBP genes were subjected to purification selection during evolution. Among them,CaFT2 and CaFT4 were relatively stable during evolution;there were eight pairs of collinearity genes between pepper and tomato. The qRT-PCR expression profile ofCaPEBP genes were significantly differentially expressed in the tissues tested. For example,CaMFT/CaTFL1-1 was highly expressed in fruits,and CaFT1 had the highest relative expression level in leaves;the expression of CaFT3 was the highest in shoot apical meristem and flower,while CaTFL1-4 was specifically expressed in roots.

Key words: Capsicum annuum, florigen, PEBP gene family, genome-wide analysis, flowering regulation, gene expression

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