关键词: 猕猴桃, 猕猴桃病毒A, 猕猴桃病毒B, 基因组, 分子变异, 病毒检测

Abstract: Kiwifruit（Actinidia spp.）viruses has seriously damaged kiwifruit production. This study investigated the incidence and distribution of Actinidia virus A（AcVA）and Actinidia virus B（AcVB）in four major kiwifruit cultivars（‘Xuxiang’，‘Hayward’，‘Huayou’and‘Qinmei’）grown in Shaanxi. The results showed that AcVA and AcVB were widely spread，with high infection rates in Shaanxi. Among the four main kiwifruit producing areas tested in this study，the highest infection rates for AcVA and AcVB were obtained in Yangling（36.7%）and Meixian（36.9%）. Among the four tested kiwifruit cultivars，the highest infection rates for AcVA（32.3%）and AcVB（32.9%）were obtained in‘Xuxiang’. This study conducted next-generation sequencing and obtained the genome sequence of AcVA-ZZ and AcVB-ZZ isolates which consisted of 7 654 and 7 454 nucleotides，respectively. The genomes of AcVA-ZZ and AcVB-ZZ isolates were 82.7% and 78.5% homologous with those of TP7-93A and TP7-93B isolates previously reported. Phylogenetic trees of AcVA and AcVB coat protein gene showed substantial sequence variations and therefore were considered as two distinct clades.

Key words: Actinidia, Actinidia virus A, Actinidia virus B, genome；molecular variability, virus detection