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园艺学报 ›› 2020, Vol. 47 ›› Issue (7): 1383-1390.doi: 10.16420/j.issn.0513-353x.2019-0760

• 研究报告 • 上一篇    下一篇

猕猴桃病毒A和B在陕西省的分布、分子变异与基因组研究

赵 磊1,张元乐1,黄倩茹1,侯彩婷1,吴 宽1,3,曹孟籍4,王乔春1,2,*,吴云锋1,*   

  1. 1西北农林科技大学植物保护学院,旱区作物逆境生物学国家重点实验室,陕西杨凌 712100;2西北农林科技大学园艺学院,陕西杨凌 712100;3杨凌职业技术学院,陕西杨凌 712100;4西南大学柑橘研究所,重庆 400712
  • 出版日期:2020-07-25 发布日期:2020-07-25
  • 基金资助:
    宁夏回族自治区重点研发计划课题(2019BBF02013);陕西省重点研发计划课题(2018NY-101,2018ZDXM-NY-058);杨凌职业技术学院科学研究基金项目(A2017029)

Distribution,Molecular Characterization and Genome Sequence of Actinidia virus A(AcVA)and Actinidia virus B(AcVB)in Shaanxi

ZHAO Lei1,ZHANG Yuanle1,HUANG Qianru1,HOU Caiting1,WU Kuan1,3,CAO Mengji4,WANG Qiaochun1,2,*,and WU Yunfeng1,*   

  1. 1State Key Laboratory of Crop Stress Biology for Arid Areas,College of Plant Protection,Yangling,Shaanxi 712100,China;2College of Horticulture,Northwest A & F University,Yangling,Shaanxi 712100,China;3Yangling Vocational & Technical College,Yangling,Shaanxi 712100,China;4Citrus Research Institute,Southwest University,Chongqing 400712,China
  • Online:2020-07-25 Published:2020-07-25

摘要: 系统调查了中国陕西省猕猴桃主产区周至、眉县、杨凌和汉中‘徐香’、‘海沃德’、‘华优’和‘秦美’猕猴桃上猕猴桃病毒A(AcVA)和猕猴桃病毒B(AcVB)的带毒率和分布情况。结果表明,AcVA和AcVB在4个产区均有广泛地分布和较高的带毒率。其中,AcVA和AcVB分别在杨凌(36.7%)和眉县(36.9%)有最高的带毒率。按照品种划分,AcVA(32.3%)和AcVB(32.9%)均在‘徐香’品种上有最高的带毒率。通过高通量测序与分析获得了AcVA和AcVB的基因组序列,其分别由7 654和7 454个核苷酸组成,与之前报道的TP7-93A和TP7-93B分离物对应的同源率分别为82.7%和78.5%。AcVA和AcVB外壳蛋白基因系统发育树显示,2种病毒分成2个组,存在较大的分子变异。

关键词: 猕猴桃, 猕猴桃病毒A, 猕猴桃病毒B, 基因组, 分子变异, 病毒检测

Abstract: Kiwifruit(Actinidia spp.)viruses has seriously damaged kiwifruit production. This study investigated the incidence and distribution of Actinidia virus A(AcVA)and Actinidia virus B(AcVB)in four major kiwifruit cultivars(‘Xuxiang’,‘Hayward’,‘Huayou’and‘Qinmei’)grown in Shaanxi. The results showed that AcVA and AcVB were widely spread,with high infection rates in Shaanxi. Among the four main kiwifruit producing areas tested in this study,the highest infection rates for AcVA and AcVB were obtained in Yangling(36.7%)and Meixian(36.9%). Among the four tested kiwifruit cultivars,the highest infection rates for AcVA(32.3%)and AcVB(32.9%)were obtained in‘Xuxiang’. This study conducted next-generation sequencing and obtained the genome sequence of AcVA-ZZ and AcVB-ZZ isolates which consisted of 7 654 and 7 454 nucleotides,respectively. The genomes of AcVA-ZZ and AcVB-ZZ isolates were 82.7% and 78.5% homologous with those of TP7-93A and TP7-93B isolates previously reported. Phylogenetic trees of AcVA and AcVB coat protein gene showed substantial sequence variations and therefore were considered as two distinct clades.

Key words: Actinidia, Actinidia virus A, Actinidia virus B, genome;molecular variability, virus detection

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