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园艺学报 ›› 2018, Vol. 45 ›› Issue (12): 2321-2330.doi: 10.16420/j.issn.0513-353x.2018-0220

• 研究论文 • 上一篇    下一篇

臭氧胁迫及恢复过程中葡萄叶片光系统活性的变化

邢 浩1,2,郝桂梅1,卞凤娥1,陈钲文1,王 辉1,翟 衡1,孙永江1,*,杜远鹏1,*   

  1. 1山东农业大学园艺科学与工程学院/作物生物学国家重点实验室,农业部黄淮地区园艺作物生物学与种质创制重点实验室,山东泰安 271018;2蓬莱市葡萄与葡萄酒局,山东蓬莱 265600
  • 出版日期:2018-12-25 发布日期:2018-12-25
  • 基金资助:
    国家自然科学基金项目(31572084);国家现代农业产业技术体系建设专项资金项目(CARS-29);长江学者和创新团队发展计划项目(IRT15R42);山东省“双一流”建设奖补资金项目(SYL2017YSTD10)

The Response of Light System Activity in Grapevine Leaves During Ozone Stress and Recovery Periods

XING Hao1,2,HAO Guimei1,BIAN Feng’e1,CHEN Zhengwen1,WANG Hui1,ZHAI Heng1,SUN Yongjiang1,*,and DU Yuanpeng1,*   

  1. 1State Key Laboratory of Crop Biology,College of Horticulture Science and Engineering,Key Laboratory of Biology and Genetic Improvement of Horticultural Crops in Huanghuai Region,Ministry of Agriculture,Shandong Agricultural University,Tai’an,Shandong 271018,China;2Penglai Vine and Wine Bureau,Penglai,Shandong 265600,China
  • Online:2018-12-25 Published:2018-12-25

摘要: 为探究臭氧(O3)胁迫及恢复过程中葡萄叶片光系统活性的响应,以1年生‘赤霞珠’葡萄(Vitis vinifera L.‘Cabernet Sauvignon’)为试材,设置臭氧 + 弱光(90 nL · L-1 O3 + 10 μmol · m-2 · s-1)、臭氧 + 适光(90 nL · L-1 + 800 μmol · m-2 · s-1)、臭氧 + 强光(90 nL · L-1 + 1 600 μmol · m-2 · s-1)3种处理,均处理3 h,以大气臭氧浓度、适光处理(10 nL · L-1 + 800 μmol · m-2 · s-1)作为对照,分别测定处理后1、5、9和13 h的叶片气体交换参数,同时利用Dual-PAM100荧光仪测定叶片PSⅠ及PSⅡ光系统活性分析臭氧胁迫及恢复过程中光系统活性的响应。结果表明:臭氧胁迫可以降低葡萄净光合速率和气孔导度,同时降低了叶片的Y(Ⅰ)和Y(Ⅱ)值,在相同臭氧浓度下,随着光照增强叶片受到的伤害加大。恢复13 h后,臭氧弱光和臭氧适光处理的叶片净光合速率、Y(Ⅰ)和Y(Ⅱ)值回升,但臭氧强光处理的叶片伤害不断加深,光系统活性不能得到恢复。

关键词: 葡萄, 臭氧, 光强, 光系统活性, 恢复

Abstract: To study the response of light system activity in grapevine leaves during ozone(O3)stress and recovery period,one-year old Vitis vinifera L.‘Cabernet Sauvignon’was used as material to be treated with ozone and low light(90 nL · L-1 + 10 μmol · m-2 · s-1),ozone and light(90 nL · L-1 + 800 μmol · m-2 · s-1),ozone and strong light(90 nL · L-1 + 1 600 μmol · m-2 · s-1). Normal light(10 nL · L-1 O3 + 800 μmol · m-2 · s-1)was as the control. The gas exchange parameters of grape leaves at 1,5,9 and 13 h after treatment were determined,and the photosystem activities of PSⅠand PSⅡ in grape leaves were measured using Dual-PAM100 fluorometer. The results showed that,the net photosynthetic rate and stomatal conductance of grape leaves were reduced under ozone stress,and the Y(Ⅰ) and Y(Ⅱ) values of grape leaves were reduced at the same time. Under the same ozone concentration,the damage of grape leaves increased with the increase of light intensity. The net photosynthetic rate,Y(Ⅰ) and Y(Ⅱ) values of dark ozone and light-resistant ozone could be increased at 13 hours after recovery,but the damage of leaves under ozone and strong light became more serious at 13 hours after recovery,and could not be recovered.

Key words: grape, ozone, light intensity, light system activity, recovery

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