园艺学报 ›› 2016, Vol. 43 ›› Issue (7): 1275-1285.doi: 10.16420/j.issn.0513-353x.2016-0145

• 蔬菜 • 上一篇    下一篇



  1. (中国农业科学院蔬菜花卉研究所,北京 100081)
  • 出版日期:2016-07-25 发布日期:2016-07-25

Fine Mapping of UI3a,a Gene Controlling Unilateral Incompatibility in Tomato

SONG Li-hua,CUI Li-peng,WANG Xiao-xuan,GAO Jian-chang,GUO Yan-mei,HUANG Ze-jun,and DU Yong-chen*   

  1. (Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences,Beijing 100081,China)
  • Online:2016-07-25 Published:2016-07-25


单向不亲和对于研究种间花粉不亲和及自交不亲和机制有重要意义。以潘那利番茄‘LA0716’渐渗系群体中的单向不亲和株系为主要材料,通过花粉萌发试验,正、反交试验和花粉管在雌蕊中的伸长研究,发现单向不亲和株系的花粉活力正常,其花粉与普通栽培番茄杂交可正常结实,但是其雌蕊不能接受自身或栽培番茄的花粉。然而,将秘鲁番茄‘LA3858’的花粉授予单向不亲和株系,花粉能够正常萌发,花粉管正常伸长和受精,但是杂交种子败育。利用20个分子标记对BC5S3群体的1 000株植株进行遗传分析,结合表型鉴定结果,将单向不亲和基因UI3a精细定位于第3号染色体上分子标记C2_At3g10220和1k-1294之间,物理距离为349 kb,该区域编码37个基因。

关键词: 番茄, 单向不亲和, UI3a基因, 精细定位


Unilateral incompatibility(UI)is a kind of incompatibility that occurs in one direction only,most often when the pollen donor is self-compatible(SC)and the recipient is self-incompatible(SI). In the present study,we identified 4 unilateral incompatibility lines(ui3a)from an introgression line(IL)population of Solanum pennellii‘LA0716in a fresh market tomato inbred line 1052 that was developed by our group. Pollen germination test showed that the pollen viability of these ui3a lines was normal. Crosses were successful when these ui3a lines as pollen donor and the self-compatibility(SC)lines from the IL population as recipient,but not vice versa. Pollen tube growth studies indicated that the pistils of these ui3a lines rejected the pollen of themselves and self-compatibility(SC)lines from the IL population,but accept the pollen of S. peruvianum accession‘LA3858. SubsequentlyUI3a gene was fine-mapped to a 349 kb region between markers C2_At3g10220 and 1k-1294 on chromosome 3. There were 37 predicted genes in this region.

Key words: tomato, unilateral incompatibility, UI3a, fine mapping