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园艺学报 ›› 2013, Vol. 40 ›› Issue (10): 2026-2032.

• 研究报告 • 上一篇    下一篇

兰属植物目标起始密码子(SCoT)遗传多样性分析

高 岭,冯尚国,何仁锋,赵妙玉,王慧中*   

  1. (杭州师范大学生命与环境科学学院,浙江省药用植物种质改良和质量监控重点实验室,杭州 310036)
  • 收稿日期:2013-06-09 出版日期:2013-10-25 发布日期:2013-10-25

Application of SCoT Molecular Marker in Genetic Diversity of Cymbidium

GAO Ling,FENG Shang-guo,HE Ren-feng,ZHAO Miao-yu,and WANG Hui-zhong*   

  1. (College of Life and Environmental Sciences,Hangzhou Normal University,Zhejiang Provincial Key Laboratory for Genetic Improvement and Quality Control of Medicinal Plants,Hangzhou 310036,China)
  • Received:2013-06-09 Online:2013-10-25 Published:2013-10-25

摘要: 应用SCoT分子标记技术对兰属14个种的24个样品进行遗传多样性分析,27条引物共扩增出259条DNA条带,其中多态性条带224条,多态性百分比达86.49%,条带大小为100 ~ 2 000 bp,多数条带分布在500 ~ 1 300 bp之间。根据SCoT标记计算的遗传距离系数得到的聚类结果可知,24种兰属植物可以分为5类:A类:春兰、建兰、蕙兰、莲瓣兰;B类:墨兰、寒兰;C类:春剑;D类:兔耳兰;E类:多花兰、文山红柱兰、虎头兰、象牙白、碧玉兰、黄蝉兰,这与传统兰属分类有所区别。

关键词: 兰属植物, 分子标记, SCoT, 遗传多样性

Abstract: In this research,SCoT markers were applied to detect the genetic diversity among 24 Cymbidum samples of 14 species. Two hundred and fifty-nine DNA bands were generated by 27 primers including 224 polymorphic bands. The polymorphic percent is 86.49%. Strip size was from 100 bp to 2 000 bp,and most of the bands were concentrated between 500 bp and 1 300 bp. Dendrograms generated from coefficient of genetic distance between species on the basis of SCoT markers showed that the germplasms were divided into 5 clusters. Cluster A was composed of C. georingiiC. ensifoliumC. faberi,and C. tortosepalum;Cluster B included C. sinese and C. kanranC. goeringii var. ongibracteatum was cluster C;C. lancifolium belonged to cluster D;Cluster E was composed of C. floribundumC. wenshanenseC. hookerianumC. maguanenseC. lowianum and C. iridioides. The research showed that part of the result was different from the traditional classification of Cymbidium.

Key words: Cymbidum;molecular marker, SCoT, genetic diversity

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