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园艺学报 ›› 2012, Vol. 39 ›› Issue (3): 567-573.

• 研究报告 • 上一篇    下一篇

5–氮杂胞苷对白菜幼苗DNA 甲基化和耐热性的影响

 许会会, 刘维信, 孙 艳, 林 多   

  1. 青岛农业大学园林园艺学院,山东青岛 266109
  • 出版日期:2012-03-25 发布日期:2012-03-25

The Effects of 5-azacytidine on DNA Methylation and Heat Tolerance of Seedlings of Non-heading Chinese Cabbage

XU Hui-Hui, LIU  Wei-Xin, SUN   Yan, LIN   Duo   

  1. College of Landscaping & Horticulture,Qingdao Agricultural University,Qingdao,Shandong 266109,China
  • Online:2012-03-25 Published:2012-03-25

摘要: 研究了不同浓度5–氮杂胞苷(5-azaC)处理白菜‘夏帝’和‘冬赏味’品种的种子,对其幼苗甲基化和耐热性的影响。结果表明:不同浓度5-azaC 溶液处理可显著降低幼苗DNA 甲基化水平,植株形态发育未见异常。0.1 ~ 0.2 mol · L-1 5-azaC 处理可减缓幼苗在高温胁迫下的生长量、POD 活性和蛋白质含量的降低幅度,同时降低MDA 含量和细胞膜透性。

关键词: 白菜, 5–氮杂胞苷, 甲基化, 高温胁迫, 耐热性

Abstract: The effects of 5-azacytidine(5-azaC)on DNA methylation levels and heat tolerance of two commercial cultivars of non-heading Chinese cabbage [Brassica campestris L. ssp. chinensis(L.)Makino],e.i. ‘Xiadi’and‘Dongshangwei’,were studied. The results showed that DNA methylation levels of seedlings of the two cultivars were significantly decreased,no abnormal growth or development was observed. Pre-treatment of seeds of non-heading Chinese cabbage with 0.1 ~ 0.2 mol · L-1 of 5-azaC would slow down the decreasing of growth,POD activity,and soluble protein content of seedlings under heat stress,and decrease MDA content and cell membrane permeability as well.

Key words: non-heading Chinese cabbage, 5-azaC, DNA methylation, heat stress, heat tolerance

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