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园艺学报 ›› 2011, Vol. 38 ›› Issue (4): 701-708.

• 蔬菜 • 上一篇    下一篇

番茄花柄离区发育基因JOINTLESS及互作蛋白基因的功能研究

王 翔1,2,尹 钧1,*   

  1. 1河南农业大学国家小麦工程技术研究中心,郑州 450002;2中国农业科学院作物科学研究所,北京 100081
  • 收稿日期:2011-01-18 修回日期:2011-03-22 出版日期:2011-04-25 发布日期:2011-04-25
  • 通讯作者: 尹 钧

Functional Studies of JOINTLESS and Its Interacting MADS-domain Proteins in Tomato

WANG Xiang 1,2 and YIN Jun 1,*   

  1. 1 National Center for Wheat Research,Henan Agricultural University,Zhengzhou 450002,China;2 Institute of Crop Sciences,Chinese Academy of Agricultural Sciences/National Key Facility for Crop Gene Resources and Genetic Improvement,Beijing 100081,China
  • Received:2011-01-18 Revised:2011-03-22 Online:2011-04-25 Published:2011-04-25
  • Contact: YIN Jun

摘要: 番茄离区发育控制基因JOINTLESS(J)是一个MADS-box家族基因。MADS-box基因是植物花发育过程中一类重要的转录因子,MADS-box蛋白调节高等植物发育过程中往往形成复合体控制基因的转录。为了研究J及其互作蛋白在番茄发育过程中的重要作用,选择7个与JOINTLESS蛋白互作的MADS-box基因进行RNAi功能研究。研究结果表明TAG1基因不仅严重影响番茄雄蕊和雌蕊的形态,还影响果实的育性。同时对J进行了过量表达分析,研究结果表明,J过量表达不但显著增加了番茄花序的分支和花的数量,还改变了果实形状。

关键词: 番茄, JOINTLESS, MADS-box基因, RNAi, 过量表达

Abstract: The MADS-box gene JOINTLESS(J)is required for flower abscission zone formation in tomato. MADS-box genes encode a class of transcription factors that usually form protein complexes controlling the transcription of genes. This study aims to identify the specific function of J and its interacting MADS-box in tomato. Seven of the JOINTLESS-interacting MADS-domain proteins are selected,and the functions of their encoding genes are studied using RNA interference(RNAi)and transgenic overexpression strategies. It is found that knockdown of TAG1 expression not only causes severe morphological changes in male and female floral organs but also reduces fertility. Overexpression of J leads to higher than normal numbers of flowers in individual inflorescences and changes in fruit shape.

Key words: tomato, JOINTLESS, MADS-box gene, RNAi, overexpression

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