园艺学报 ›› 2008, Vol. 35 ›› Issue (7): 1011-1016.

• 蔬菜 • 上一篇    下一篇




  1. 1中国农业科学院蔬菜花卉研究所,北京 100081;2上海市农业科学院园艺所,上海 201106)
  • 收稿日期:2008-03-05 修回日期:2008-05-26 出版日期:2008-07-25 发布日期:2008-07-25
  • 通讯作者: 杜永臣

Identification of Differentially Expressed Genes by cDNA-AFLP Approach During Heat Stress in Tomato Leaves

LIU Zhi-yong1,2, DU Yong-chen1*, WANG Xiao-xuan1, GUO Yan-mei1, and GAO Jian-chang1

  1. 1Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China;2Horticultural Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201106, China)
  • Received:2008-03-05 Revised:2008-05-26 Online:2008-07-25 Published:2008-07-25
  • Contact: DU Yong-chen

摘要: 为鉴定番茄高温胁迫响应基因,应用cDNA-AFLP技术,以番茄耐热品系Saladette幼苗为研究对象,对高温胁迫早期叶片基因表达进行了mRNA指纹分析。通过768对引物组合的筛选,共分离得到187个差异表达的转录衍生片段(TDF),其中增强表达或高温胁迫下特异性表达TDF153条,抑制表达34条。对其中47个TDF进行了克隆、测序和序列分析。结果表明:34个TDF与NCBI或TIGR已有序列同源(E-Value<10-5),功能涉及信号传导、转录调控以及逆境诱导蛋白等,另有13个TDF无同源序列或同源性低,预测是一些未知功能基因。

关键词: 番茄, 高温胁迫, cDNA-AFLP, 差异表达基因

Abstract: In order to identify genes involved in heat shock responses in tomato, cDNA-AFLP approach was employed to identify genes differentially expressed in tomato leaves at high temperature. 768 primer combinations were used for selective amplification. 187 TDFs were selected for their differential expression under heat stress. Among the 187 TDFs, 153 TDFs were up-regulated and 34 TDFs were suppressed. 47 TDFs were cloned and sequenced. Compared with the publicly available databases, 34 TDFs presented some significant similarity with known plant sequences, whose functions are involved in gene regulation, transcription factor, signal transduction, stress defense,etc. 13 TDFs with low identity and no match to known genes may represent novel tomato genes.

Key words: tomato (Solanum lycopersicum), heat stress, cDNA-AFLP, differentially expressed genes