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园艺学报 ›› 2007, Vol. 34 ›› Issue (5): 1177-1182.

• 蔬菜 • 上一篇    下一篇

黄瓜无苦味基因bi的分子标记研究

池秀蓉;顾兴芳*;张圣平;王晓武;王 烨
  

  1. (中国农业科学院蔬菜花卉研究所,北京100081)
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2007-10-25 发布日期:2007-10-25

Identification of Molecular Markers Linked to Foliage Non-bitterness Gene(bi)in Cucumis sativus L.

CHI Xiu-rong;GU Xing-fang*;ZHANG Sheng-ping; WANG Xiao-wu;WANG Ye   

  1. (Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2007-10-25 Published:2007-10-25

摘要: 以黄瓜纯合自交系9110Gt(bibi)和03828(BiBi)为双亲获得的F2代为试材,利用分离群体组群分析法,从256对AFLP选择性引物中获得了与营养器官无苦味位点连锁距离为6.43 cM的AFLP分子标记TG/GCT150,并成功地将其转化为操作简便、表现稳定的SCAR标记SC87。

关键词: 黄瓜, 苦味, 分子标记, AFLP, SCAR

Abstract: In this study, AFLP technique and the method of bulked segregant analysis were used to study the F2 progeny of purified cucumber line 9110Gt(bibi)×03828(BiBi).One AFLP molecular markers TG/GCT150 linked to non-bitterness gene in cucumber with a genetic distance 6.43 cM were identified from 256 primer combinations. This band was collected and sequenced to synthesize a sequence characterized amplified region (SCAR) primer, and TG/GCT150 was converted to SCAR marker SC87 successfully.

Key words: Cucumber, Bitterness, Molecular marker, AFLP, SCAR