园艺学报 ›› 2006, Vol. 33 ›› Issue (6): 1345-1348.

• 研究报告 • 上一篇    下一篇

白菜GLDH 基因cDNA的克隆及序列分析

任锡亮1;侯喜林1, 2;李英2   

  1. (1 南京农业大学作物遗传与种质创新国家重点实验室, 江苏南京210095; 2 南京农业大学园艺学院, 江苏南京210095)
  • 收稿日期:2005-12-19 修回日期:2006-04-13 出版日期:2006-12-25 发布日期:2006-12-25
  • 通讯作者: 侯喜林

Molecular Clon ing and Sequence Analysis of GLDH Gene cDNA from Brassica campestris ssp. chinensis

Ren Xiliang1;Hou Xilin1, 2;Li Ying2

  1. (1National Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing, Jiangsu 210095, China; 2College of Horticulture, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China)
  • Received:2005-12-19 Revised:2006-04-13 Online:2006-12-25 Published:2006-12-25
  • Contact: Hou Xilin

摘要: 以白菜品种‘苏州青’ cDNA 为模板, 采用RT-PCR、巢式PCR、3’RACE和5’RACE技术, 获得了L - 半乳糖酸- 1, 4 - 内酯脱氢酶(EC, GLDH) 基因cDNA 2 034 bp全长序列。序列分析表明, GLDH基因cDNA序列编码601个氨基酸。其氨基酸序列与花椰菜GLDH基因具有98%的同源性, 与拟南芥GLDH基因具有90%的同源性。该基因在GenBank中登录号为AY899298。

关键词: 白菜, L - 抗坏血酸, L - 半乳糖酸- 1, 4 - 内酯脱氢酶

Abstract: The L-galactono-1, 4-lactone dehydrogenase ( EC, GLDH) is a key enzyme that catalyzes the final step in the L-ascorbic acid synthetic pathway of plants. GLDH gene cDNA sequence was cloned with cDNA isolated from Brassica campestris ssp. chinensis‘Suzhouqing’. A 2 034 bp full-length cDNA sequence of GLDH was obtained using RT-PCR, nested PCR, 3 ’RACE and 5 ’RACE techniques.Further analysis on GLDH gene cDNA indicated that it encoded a peptide containing 601 amino acids. The amino acid sequence comparison with Brassica oleracea GLDH gene and Arabidopsis thaliana GLDH gene showed that identity was 98% and 90% , respectively. The sequence was accepted and released by GenBank (Accession number AY899298).

Key words: Brassica campestris ssp. chinensis Makino, L-ascorbic acid, L-galactono-1, 4-lactone dehydrogenase