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https://www.ahs.ac.cn/images/0513-353X/images/top-banner6.jpg|#|黄瓜
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园艺学报 ›› 2003, Vol. 30 ›› Issue (1): 47-50.

• 研究论文 • 上一篇    下一篇

黄瓜栽培种、近缘野生种、种间杂种及其回交后代的RAPD 分析

庄飞云;陈劲枫*   

  1. (作物遗传与种质创新国家重点实验室, 南京农业大学蔬菜研究所, 南京210095)
  • 收稿日期:2002-01-23 修回日期:2002-06-03 出版日期:2003-02-25 发布日期:2003-02-25

RAPD Analysis of Cultivated Cucumber,Wild Cucumis Species, Interspecific Hybrid and Its Progenies from Backcrossing

Zhuang Feiyun;Chen Jinfeng   

  1. (National Key Laboratory of Plant Genetics and Breeding , Vegetable Research Institute , Nanjing Agricultural University , Nanjing 210095 , China)
  • Received:2002-01-23 Revised:2002-06-03 Online:2003-02-25 Published:2003-02-25

摘要: 黄瓜栽培种、黄瓜野生变种、近缘野生种、种间杂交种及其与黄瓜回交自交的后代以及甜瓜为试材, 采用RAPD 方法探讨其亲缘关系。对23 份试材进行扩增, 筛选出31 条随机引物的扩增产物进行结果分析, 共产生375 个位点, 具多态性位点占90. 0 % , 平均每条扩增出6. 3 条。通过UPGMA 聚类分析,以遗传距离0. 37 为阀值, 可将23 份试材归为黄瓜、近缘野生种、种间杂交种及甜瓜亚属种4 类。

关键词: 黄瓜, 种间杂交, 亲缘关系, RAPD

Abstract: Cultivated cucumber and its wild varieties , wild Cucumis species ( C. hystrix Chakr. ) ,interspecific hybrids and the BC1S2 , and melon cultivars were used to study the relationship by RAPD markers. 31 arbitrary primers were screened to detected polymorphism among the total 23 cultigens used. Of the 375 sites
produced , 90. 0 % were polymorphic. Each primer amplified an average of 6. 3 fragments. The results from UPGMA cluster analysis suggested that 23 cultigens could be classified into four groups , they are : cultivated cucumber and its wild variety , C. hystrix , C. ×hytivus , and melons.

Key words: Cucumis, Interspecific hybridization, Phylogeny, RAPD

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