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园艺学报 ›› 2003, Vol. 30 ›› Issue (1): 43-46.

• 研究论文 • 上一篇    下一篇

试管芋诱导的研究

刘玉平;柯卫东;黄新芳;彭静   

  1. (武汉市蔬菜科学研究所水生蔬菜研究室, 武汉430065)
  • 收稿日期:2001-11-05 修回日期:2002-07-12 出版日期:2003-02-25 发布日期:2003-02-25

Induction of in Vitro Corms of Taro ( Colocasia esculenta Schott. )

Liu Yuping;Ke Weidong;Huang Xinfang;Peng Jing   

  1. ( The Department of Aquatic Vegetable , Wuhan Institute of Vegetable Science , Wuhan 430065 , China)
  • Received:2001-11-05 Revised:2002-07-12 Online:2003-02-25 Published:2003-02-25

摘要: 以芋组培苗为试材, 研究了蔗糖浓度、激素浓度、光照时间、培养温度、不同大小试管苗,不同芋品种类型等因素对试管芋诱导的影响及不同基质对试管芋育苗成活率的影响。结果表明: 诱导试管芋较理想的培养基为MS + 蔗糖8 % + BA 1. 0 mg·L - 1 + NAA 0. 5 mg·L-1 , 光照12 h/ d , 培养温度30 ℃; 试管苗越大越有利于试管芋的形成; 试管芋可保持其原有的特性; 基质影响试管芋育苗的成活率, 4 种基质中蛭石最好。

关键词: 芋, 试管苗, 诱导, 育苗

Abstract: The effects of concentration of sucrose and hormone,photoperiod,temperature, the size of plantlets and the types of cultivars on the induction of in vitro corms and the effects of cultural bases on the survial ratio of the transplanted plants in vitro were studied with the plantlets cultured from taro ( Colocasia esculenta
Schott . ) shoot tips. The results are as follows: The suitable cultural medium for inducing in vitro corms is MS + sucrose 8 % +BA 1. 0 mg·L -1 + NAA 0. 5 mg·L-1 ; The suitable cultural conditions is 12 h/ d light , 30 ℃; The bigger the size of the plantlets are , the easier the in vitro corms are to be induced ; The characters of taro in vitro corms induced are in accordance with the types of the cultivars; The survial ratio are affected by the cultural bases, among which the vermiculite is the best.

Key words: Taro ( Colocasia esculenta Schott.), Tube plantlets, Induction, Seedling culture

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