葡萄VvSWEET蛋白家族的全基因组鉴定及VvSWEET15在糖含量积累的功能研究

doi:10.16420/j.issn.0513-353x.2025-0792

园艺学报 ›› 2026, Vol. 53 ›› Issue (7): 2019-2033.doi: 10.16420/j.issn.0513-353x.2025-0792

• 遗传育种 · 种质资源 · 分子生物学 • 上一篇下一篇

葡萄VvSWEET蛋白家族的全基因组鉴定及VvSWEET15在糖含量积累的功能研究

鄢云¹^,^*, 肖阳¹^,^*, 姚雅淇¹, 侬玉英¹, 郭得君², 邵建辉³, 马春花¹, 苏静¹^,^**()

¹ 云南农业大学园林园艺学院，昆明 650201
² 甘肃农垦永昌农场有限公司，甘肃永昌 737200
³ 云南农业大学植物保护学院，昆明 650201

收稿日期:2025-08-26 修回日期:2026-04-19 出版日期:2026-07-25 发布日期:2026-07-23
通讯作者:
^** E-mail：2023020@ynau.edu.cn
作者简介:
^* 共同第一作者
基金资助:
国家自然科学基金地区项目(32460720); 国家自然科学基金地区项目(32160691); 国家自然科学基金地区项目(32360739); 云南省科技人才与平台计划项目(202505AS350015); 云南省科技厅农业联合项目(202301BD070001-020)

Genome-Wide Identification of Grapevine SWEET Protein Family Reveals the Potential Function of VvSWEET15 in Sugar Accumulation

YAN Yun¹, XIAO Yang¹, YAO Yaqi¹, NONG Yuying¹, GUO Dejun², SHAO Jianhui³, MA Chunhua¹, SU Jing¹^,^**()

¹ College of Landscape Architecture and Horticulture， Yunnan Agricultural University， Kunming 650201， China
² Gansu Nongken Yongchang Farm Co.， Ltd.，Yongchang， Gansu 737200， China
³ College of Plant Protection， Yunnan Agricultural University， Kunming 650201， China

Received:2025-08-26 Revised:2026-04-19 Published:2026-07-25 Online:2026-07-23

摘要/Abstract

摘要：

SWEET（Sugars Will Eventually be Exported Transporters）家族是近年来新发现的一类糖转运蛋白，调节糖类化合物在植物体内卸载及积累，参与植物衰老、花蜜分泌、激素信号、发病机制和非生物胁迫响应等过程。本研究中基于葡萄（Vitis vinifera L.）全基因组信息对糖转运蛋白SWEET家族成员进行鉴定，分析其在果实不同发育时期的表达特征，旨在挖掘调控果实糖积累的关键基因。通过生物信息学方法鉴定VvSWEET基因，系统分析其理化性质、进化关系、染色体定位、基因结构以及启动子顺式作用元件等特征。结合‘红地球’葡萄不同发育时期果实转录组数据，筛选出与己糖含量积累呈显著正相关的基因VvSWEET15，并通过基因克隆、亚细胞定位和基因沉默等技术，明确VvSWEET15在果实糖积累过程中的功能。共鉴定到16个VvSWEET家族成员，主要分布在10条染色体上。分析表明，葡萄VvSWEET基因可分为4个进化分支（CladeⅠ、Ⅱ、Ⅲ和Ⅳ），同一分支成员在基因结构和蛋白保守基序上高度相似。启动子区域富含光照、激素、发育和胁迫相关的顺式作用元件。分析表明，花后40 ~ 120 d VvSWEET15表达水平呈上升趋势，并与果实中己糖含量呈显著正相关。VvSWEET15定位于细胞质膜，瞬时沉默其表达显著降低了果实中果糖和葡萄糖含量，表明VvSWEET15对果实己糖积累起重要作用。

关键词: 葡萄, 糖转运蛋白, VvSWEET, 进化分析, 糖积累

Abstract:

SWEET is a newly discovered class of sugar transporters that regulate the unloading and accumulation of sugar in plants and participate in plant senescence，nectar secretion，hormone signaling，pathogenesis，and abiotic stress response. The study systematically identifies the VvSWEETs protein family in grapevine（Vitis vinifera L.）and analyses its expression patterns across different stages of berry development to explore genes related to sugar accumulation in grape berries，which is crucial for improving fruit quality. The physicochemical properties，phylogenetic relationships，gene structure，and cis-regulatory elements of VvSWEETs were analyzed using grape whole-genome data. Integrating these analyses with transcriptome data from‘Red Globe’at different developmental stages，VvSWEET15 was identified as a key candidate. Its function in fruit sugar accumulation was elucidated through gene cloning，subcellular localization，and gene silencing. The grape genome contains 16 VvSWEET genes，distributed on 10 chromosomes and categorized into four evolutionary clades（CladesⅠ，Ⅱ，Ⅲ and Ⅳ）. Genes within each clade share similar structural characteristics. The promoter region is enriched in cis-acting elements responsive to light, phytohormones, development, and stress. Transcriptome analysis revealed increased VvSWEET15 expression from 40 to 120 days after flowering，positively correlating with hexose accumulation in ripe berries. VvSWEET15 is a protein localized to the plasma membrane，and transient silencing of its expression significantly reduced the fructose and glucose concentrations in fruits，indicating that VvSWEET15 plays an important role in the accumulation of sugar concentration in grape fruits.

Key words: Vitis vinifera, sugar transporter, VvSWEET, phylogenetic analysis, sugar accumulation

鄢云, 肖阳, 姚雅淇, 侬玉英, 郭得君, 邵建辉, 马春花, 苏静. 葡萄VvSWEET蛋白家族的全基因组鉴定及VvSWEET15在糖含量积累的功能研究[J]. 园艺学报, 2026, 53(7): 2019-2033.

YAN Yun, XIAO Yang, YAO Yaqi, NONG Yuying, GUO Dejun, SHAO Jianhui, MA Chunhua, SU Jing. Genome-Wide Identification of Grapevine SWEET Protein Family Reveals the Potential Function of VvSWEET15 in Sugar Accumulation[J]. Acta Horticulturae Sinica, 2026, 53(7): 2019-2033.

图/表 10

表1 VvSWEET蛋白家族的理化特征

Table 1 The physicochemical characteristics of the VvSWEET protein family

基因ID Gene ID	位置与长度 Location and length	编码序列/bp CDS	等电点 pI	分子量/kD Molecular weight	氨基酸残基数量 No. of amino acids	总平均亲水性 Grand average of hydropathy	脂肪系数 Aliphatic index	不稳定系数Instability index
Vitvi18g01215	chr18:13433660..13436779 (3.12 kb)	747	9.64	27.35	248	0.682	111.29	28.21
Vitvi19g00024	chr19:535653..539306 (3.65 kb)	708	9.30	26.12	235	0.741	117.32	47.26
Vitvi10g00679	chr10:8728270..8732553 (4.28 kb)	699	8.84	25.78	232	0.839	115.99	53.21
Vitvi16g01984	chr16:20108856..20111853 (3 kb)	750	9.13	28.04	249	0.535	116.14	39.81
Vitvi14g01783	chr14:28363163..28366829 (3.67 kb)	775	9.36	27.90	254	0.517	108.98	38.85
Vitvi17g00791	chr17:9893489..9895615 (2.13 kb)	705	9.43	26.16	234	0.693	124.06	29.69
Vitvi17g00793	chr17:9939039..9941147 (2.11 kb)	699	9.49	25.97	232	0.698	119.66	30.31
Vitvi02g00181	chr02:1665640..1669158 (3.52 kb)	783	9.62	28.85	260	0.695	116.54	43.99
Vitvi04g01075	chr04:15667871..15670837 (2.97 kb)	837	9.05	31.52	278	0.576	112.16	39.12
Vitvi17g00070	chr17:1349304..1351901 (2.6 kb)	903	7.63	33.96	300	0.745	126.63	38.56
Vitvi07g00250	chr07:2765683..2768134 (2.45 kb)	831	9.30	31.00	276	0.535	106.05	34.08
Vitvi17g00069	chr17:1344616..1347770 (3.15 kb)	852	9.34	31.70	283	0.575	112.93	38.63
Vitvi01g01719	chr01:23088842..23091312 (2.47 kb)	870	9.08	32.15	289	0.492	110.00	35.66
Vitvi14g00149	chr14:1256163..1261104 (4.94 kb)	897	9.68	32.76	298	0.424	107.58	37.96
Vitvi14g00148	chr14:1248783..1251155 (2.37 kb)	717	6.90	26.47	238	0.782	124.79	46.38
Vitvi14g00147	chr14:1240096..1242964 (2.87 kb)	714	6.29	26.27	237	0.845	128.23	48.06

图1 葡萄和拟南芥SWEET蛋白家族成员系统发育树

Fig. 1 Phylogenetic tree of SWEET protein family members in Vitis vinifera and Arabidopsis

图2 VvSWEET基因的染色体定位

Fig. 2 The chromosomal location of the VvSWEET

图3 VvSWEET蛋白的保守基序及其结构分析

Fig. 3 The conserved motifs and gene structure of VvSWEET

图4 葡萄VvSWEET基因启动子顺式作用元件分析

Fig. 4 Analysis of the cis-acting elements in the promoter of the VvSWEET gene in grapevine

图5 VvSWEET基因在葡萄不同发育时期的表达特性（A）及其与果糖和葡萄糖相关性分析（B） S1 ~ S4时期分别为花后40、80、100和120 d；*** 表示在P ≤ 0.001水平存在显著相关。

Fig. 5 Expression profiles correlation analysis of VvSWEET in different fruit development stages of grapevine（A）and correlation analysis with fructose and glucose（B） S1 to S4 correspond to 40，80，100，and 120 days after flowering，respectively；*** indicates a significant correlation at P ≤ 0.001

图6 VvSWEET15基因的克隆

Fig. 6 Cloning of VvSWEET15 gene

图7 VvSWEET15测序比对

Fig. 7 Sequence comparison of VvSWEET15 gene

图8 VvSWEET15蛋白亚细胞定位

Fig. 8 Subcellular localization analysis of VvSWEET15

图9 VIGS介导沉默VvSWEET15的表达对葡萄果实中果糖和葡萄糖含量的影响数值由平均值 ± 标准误（n = 3）表示，**表示在P ≤ 0.01水平存在显著差异

Fig. 9 Effect of VIGS-mediated silencing of VvSWEET15 on fructose and glucose content in grape berry Bars represent the mean value ± SE（n = 3）. ** indicates a significant difference at P ≤ 0.01

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葡萄VvSWEET蛋白家族的全基因组鉴定及VvSWEET15在糖含量积累的功能研究

Genome-Wide Identification of Grapevine SWEET Protein Family Reveals the Potential Function of VvSWEET15 in Sugar Accumulation

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