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园艺学报 ›› 2026, Vol. 53 ›› Issue (3): 763-774.doi: 10.16420/j.issn.0513-353x.2025-0277

• 栽培·生理生化 • 上一篇    下一篇

‘金奉’猕猴桃实生种质溃疡病、软腐病抗性及果实品质评价

陈艳燕1,2, 乐晨曦1, 涂慧1, 师冀康1, 陶俊杰1, 潘慧2, 李文艺2, 李黎2,*(), 黄春辉1,*()   

  1. 1 江西农业大学农学院,南昌 330045
    2 中国科学院武汉植物园,中国科学院植物种质创新与特色农业重点实验室,中国科学院猕猴桃产业技术工程实验室,武汉 430074
  • 收稿日期:2025-06-23 修回日期:2025-10-23 出版日期:2026-03-25 发布日期:2026-03-20
  • 通讯作者:
    * E-mail:
  • 基金资助:
    江西省重点研发计划项目(20232BBF60004); 国家自然科学基金面上项目(32272506); 贵州省科技重大专项项目(黔科合重大专项字[2024]026); 湖北省第四批现代农业产业技术体系专项资金资助(2023HBSTX4-08); 现代农业产业技术体系建设专项资助(CARS-26); 湖北省支持种业高质量发展资金农业种质资源保护利用课题(HBZY2023A001-05)

Evaluation of Resistance to Bacterial Canker Disease and Fungal Soft Rot and Fruit Quality of Jinfeng Kiwifruit Seed Offspring

CHEN Yanyan1,2, YUE Chenxi1, TU Hui1, SHI Jikang1, TAO Junjie1, PAN Hui2, LI Wenyi2, LI Li2,*(), HUANG Chunhui1,*()   

  1. 1 College of Agriculture,Jiangxi Agricultural University,Nanchang 330045,China
    2 Wuhan Botanical Garden,Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture,Engineering Laboratory for Kiwifruit Industrial Technology,Chinese Academy of Sciences,Wuhan 430074,China
  • Received:2025-06-23 Revised:2025-10-23 Published:2026-03-25 Online:2026-03-20

摘要:

以40份已结果的‘金奉’猕猴桃实生种质为试材,运用离体枝条人工接种溃疡病病菌的方法评价溃疡病抗性,离体果实人工接种软腐病病菌方法评价软腐病抗性;同时检测果形指数、可溶性固形物含量、干物质含量、维生素C含量、糖酸含量等9个指标,分析评价果实品质。基于枝条溃疡病病斑长度和果实软腐病斑直径,可将40份‘金奉’猕猴桃实生种质划分为抗病、耐病、感病及高感4个级别;其中溃疡病抗病种质11份,耐病种质17份,感病种质7份,高感种质5份;软腐病抗病种质13份,耐病种质14份,感病种质9份,高感种质4份;兼具溃疡病、软腐病抗病种质5份。相关性分析表明,果实软腐病抗性与可溶性固形物、干物质及可溶性糖含量等3个关键品质指标间显著相关。通过主成分分析法及因子分析法建立果实品质综合评价模型后,对40份‘金奉’实生种质进行果实品质优劣排序,前5位的种质依次为4-266、8-396、2-112、1-77、5-325,结合溃疡病、软腐病抗性表现,最终筛选出3份高抗病且品质优良的种质(4-266、2-112、1-77)。

关键词: 猕猴桃, 溃疡病, 软腐病, 种质, 抗性评价, 果实品质

Abstract:

This study utilized 40 fruiting‘Jinfeng’kiwifruit seedling accessions as test materials,the resistance to bacterial canker was evaluated by artificial inoculation of detached shoots with the pathogen,and the resistance to soft rot was evaluated by artificial inoculation of detached fruits with the pathogen. Simultaneously,nine indicators including fruit shape index,soluble solids content,dry matter content,vitamin C content,and sugar-acid content were measured to analyze and evaluate fruit quality. Based on the length of canker lesions on shoots and the diameter of soft rot lesions on fruits,the 40‘Jinfeng’kiwifruit seedling accessions were classified into 4 levels:resistant,tolerant,susceptible,and highly susceptible. Among them,11 accessions were resistant to canker,17 were tolerant,7 were susceptible,and 5 were highly susceptible. For soft rot,13 accessions were resistant,14 were tolerant,9 were susceptible,and 4 were highly susceptible. Five accessions exhibited resistance to both canker and soft rot. Correlation analysis revealed significant correlations between resistance to fruit soft rot and 3 key quality indicators:soluble solids content,dry matter content,and soluble sugar content. After establishing a comprehensive fruit quality evaluation model using principal component analysis and factor analysis,the fruit quality of the 40‘Jinfeng’seedling accessions was ranked. The top 5 accessions,in order,were 4-266,8-396,2-112,1-77,and 5-325. Combining the resistance performance to canker and soft rot of the top five ranked accessions,three accessions(4-266,2-112,1-77)were ultimately selected as highly disease-resistant and high-quality germplasm.

Key words: kiwifruit, bacterial canker, soft rot, germplasm resources, resistance evaluation, fruit quality