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园艺学报 ›› 2026, Vol. 53 ›› Issue (3): 775-790.doi: 10.16420/j.issn.0513-353x.2025-0093

• 栽培·生理生化 • 上一篇    下一篇

马铃薯耐低氮种质资源筛选与氮效率分析

许国春, 许泳清, 李华伟, 纪荣昌, 罗文彬, 林赵淼, 邱思鑫, 汤浩*()   

  1. 福建省农业科学院作物研究所,农业农村部南方薯类科学观测实验站,福建省特色旱作物品种选育工程技术研究中心,福州 350013
  • 收稿日期:2025-10-15 修回日期:2025-12-04 出版日期:2026-03-25 发布日期:2026-03-20
  • 通讯作者:
    * E-mail:
  • 基金资助:
    福建省属公益类科研院所基本科研专项(2023R1029007); “5511”协同创新工程项目(XTCXGC2021005); 福建省种业创新与产业化工程项目(zycxny2021005); 现代农业产业技术体系建设专项资助(CARS-09-ES11)

Screening of Potato Germplasms for Low Nitrogen Tolerance and Analysis of Nitrogen Efficiency

XU Guochun, XU Yongqing, LI Huawei, JI Rongchang, LUO Wenbin, LIN Zhaomiao, QIU Sixin, TANG Hao*()   

  1. Scientific Observing and Experimental Station of Tuber and Root Crops in South China,Ministry of Agriculture and Rural Affairs,Fujian Engineering Research Center for Characteristic Upland Crops Breeding,Crop Research Institute,Fujian Academy of Agricultural Sciences,Fuzhou 350013,China
  • Received:2025-10-15 Revised:2025-12-04 Published:2026-03-25 Online:2026-03-20

摘要: 筛选耐低氮和氮高效种质对培育马铃薯绿色高效新品种具有重要意义。采用盆栽营养液控制试验方法,对180份马铃薯种质资源在低氮(0.1 mmol · L-1 NH4NO3)和正常氮(10 mmol · L-1 NH4NO3)处理下的叶片气体交换参数、相对叶绿素含量、地上部干物质积累和产量相关性状进行测定,分析各性状变异、相关性和低氮敏感性,筛选耐低氮和氮高效种质,并进行生理机制探讨和田间验证。结果表明,180份马铃薯种质资源的光合与产量相关性状变异显著,在正常氮和低氮处理下,9个性状的变异系数分别介于11.76% ~ 73.04%和13.48% ~ 112.13%之间;而且低氮处理下各性状的变异高于正常氮处理。测定性状两两之间多数具有较明显的相关关系,且在一定程度上受氮处理影响。低氮显著影响马铃薯叶片光合和产量性状,其中单株产量受低氮抑制最明显,其变异也最大,单株产量的耐低氮指数变异系数为57.53%。通过对单株产量耐低氮指数进行聚类,筛选获得10份耐低氮种质,其中7份属于双高效基因型(CIP8、CIP52、E33、CIP10、CIP16、T1和CIP66);在田间低氮条件下,耐低氮且氮高效种质同样具有较高的产量水平。分析发现,耐低氮种质比敏感种质具有更高的净光合速率、叶绿素含量、地上部干物质量和单株结薯数。本研究结果可为进一步挖掘优异基因资源和选育高产氮高效品种提供材料基础。

关键词: 马铃薯, 种质资源, 耐低氮种质, 氮效率, 产量, 盆栽试验

Abstract:

Screening for germplasm with tolerance to low nitrogen and high nitrogen efficiency (NE)is of great significance for breeding green and high-yielding new varieties of potatoes. In this study,a pot experiment with nutrient solution control was conducted to measure the leaf gas exchange parameters,relative chlorophyll content,aboveground dry matter accumulation,and yield-related traits of 180 potato germplasms under low nitrogen(0.1 mmol · L-1 NH4NO3,LN)and normal nitrogen(10 mmol · L-1 NH4NO3, NN)treatments. The study analyzed the variation,correlation,and low-nitrogen sensitivity of each trait,screened for low nitrogen tolerant and high NE germplasms,and conducted physiological mechanism analysis and field validation experiment. The results showed that,there was significant variation in photosynthesis and yield-related traits among the 180 potato germplasms. Under NN and LN treatments,the coefficient of variation for the 9 traits ranged from 11.76% to 73.04% and 13.48% to 112.13%,respectively. Moreover,the variation in traits under LN treatment was higher than under NN treatment. Most of the traits showed a clear correlation with each other,and to some extent,were influenced by nitrogen treatment. Low nitrogen significantly affected the leaf photosynthesis and yield-related traits of potatoes,with single plant yield(SPY)being the most suppressed by low nitrogen,but also showing the greatest variation of low nitrogen tolerance index(LNTI),with a coefficient of variation of 57.53%. By clustering the LNTI of SPY,10 low nitrogen tolerant germplasms were selected,of which CIP8,CIP52,E33,CIP10,CIP16,T1 and CIP66 had high NE both under LN and NN treatment (DNHE);under field conditions of low nitrogen,DNHE germplasms also had higher yield. Analysis found that,low nitrogen tolerant germplasms had higher net photosynthesis rates,chlorophyll content,aboveground dry matter,and tuber number per plant than sensitive germplasms. The results of this study can offer valuable materials for further exploration of excellent genetic resources and the breeding of varieties with high-yield and high NE.

Key words: potato, germplasm, low nitrogen tolerant germplasm, nitrogen efficiency, yield, pot experiment