甜瓜TIFY基因家族成员鉴定及低温胁迫响应表达分析

doi:10.16420/j.issn.0513-353x.2025-0225

园艺学报 ›› 2026, Vol. 53 ›› Issue (5): 1343-1356.doi: 10.16420/j.issn.0513-353x.2025-0225

• 遗传育种·种质资源·分子生物学 • 上一篇下一篇

甜瓜TIFY基因家族成员鉴定及低温胁迫响应表达分析

曹磊¹^,^*, 杜平²^,^*, 羊日令¹, 周宇豪¹, 侯娟¹, 李翔¹, 王盼乔¹, 李丽丽¹, 罗陈¹, 李琼¹, 毛文文¹^,^**(), 胡建斌¹^,^**()

¹ 河南农业大学园艺学院，河南省瓜类种质资源创新与利用工程技术研究中心，郑州 450046
² 中国林业科学研究院经济林研究所，郑州 450003

收稿日期:2025-12-29 修回日期:2026-03-19 出版日期:2026-05-26 发布日期:2026-05-26
通讯作者:
^** E-mail：jianbinhu@henau.edu.cn，
maowenwen@henau.edu.cn
作者简介:
*共同第一作者
基金资助:
国家自然科学基金项目(32302529); 国家自然科学基金项目(32472740); 中国博士后科学基金第75批面上资助项目(2024M750813); 河南省高等学校重点科研项目(24A210012)

Identification and Low Temperature Stress Responsive Expression Analysis of TIFY Gene Family in Melon

CAO Lei¹, DU Ping², YANG Riling¹, ZHOU Yuhao¹, HOU Juan¹, LI Xiang¹, WANG Panqiao¹, LI Lili¹, LUO Chen¹, LI Qiong¹, MAO Wenwen¹^,^**(), HU Jianbin¹^,^**()

¹ Research Center of Cucurbit Germplasm Enhancement and Utilization of Henan Province， College of Horticulture，Henan Agricultural University， Zhengzhou 450046， China
² Research Institute of Non-timber Forestry， Chinese Academy of Forestry， Zhengzhou 450003， China

Received:2025-12-29 Revised:2026-03-19 Published:2026-05-26 Online:2026-05-26
Contact:
^** E-mail：jianbinhu@henau.edu.cn，
maowenwen@henau.edu.cn

摘要/Abstract

摘要：

为了研究TIFY基因家族在甜瓜（Cucumis melo L.）低温胁迫响应中的作用，利用生物信息方法对甜瓜中TIFY家族成员进行全基因组鉴定，并结合转录组数据和实时荧光定量PCR技术，分析其在各组织及低温胁迫下的表达模式。结果表明，甜瓜中共鉴定到15个TIFY家族基因，可分为JAZ、TIFY、ZIM和PPD四个亚家族，分布在9条染色体上。组织表达分析显示，CmTIFY在不同组织部位中均有表达，其中CmTIFY5A、CmTIFY5B、CmTIFY5C、CmTIFY5D和CmTIFY9B在花器官中的表达量高于其他组织。随着果实成熟，CmTIFY5B基因表达量逐渐上升，而CmTIFY6B逐渐下降，CmTIFY5B与CmTIFY6B可能参与甜瓜果实成熟及品质形成的调控。低温胁迫下，多数CmTIFY基因表达量上调，其中CmTIFY5B和CmTIFY5C的上调最为显著。

关键词: 甜瓜, 非生物胁迫, TIFY, 基因家族, 表达分析, 低温胁迫

Abstract:

To investigate the role of the TIFY gene family in the low temperature stress response of melon（Cucumis melo L.），genome-wide identification of TIFY family members was performed using bioinformatics approaches. Tissue-specific expression patterns and low temperature stress responsive profiles were further analyzed through transcriptome sequencing and quantitative reverse transcription polymerase chain reaction technology. The results demonstrated that a total of 15 TIFY family genes were identified in melon，classified into four subfamilies：JAZ，TIFY，ZIM，and PPD，and distributed across nine chromosomes. Tissue-specific expression analysis revealed that CmTIFY genes were expressed in various tissues. Among them，CmTIFY5A，CmTIFY5B，CmTIFY5C，CmTIFY5D and CmTIFY9B exhibited higher expression levels in flowers compared to other tissues. With fruit ripening，the gene expression level of CmTIFY5B gradually increased，whereas that of CmTIFY6B gradually decreased. CmTIFY5B and CmTIFY6B may be involved in the regulation of melon fruit ripening and quality formation. The expression levels of most CmTIFY genes were up regulated under low temperature stress，among which CmTIFY5B and CmTIFY5C were most significantly up regulated under low temperature.

Key words: melon, abiotic stress, TIFY, gene family, expression analysis, low temperature stress

曹磊, 杜平, 羊日令, 周宇豪, 侯娟, 李翔, 王盼乔, 李丽丽, 罗陈, 李琼, 毛文文, 胡建斌. 甜瓜TIFY基因家族成员鉴定及低温胁迫响应表达分析[J]. 园艺学报, 2026, 53(5): 1343-1356.

CAO Lei, DU Ping, YANG Riling, ZHOU Yuhao, HOU Juan, LI Xiang, WANG Panqiao, LI Lili, LUO Chen, LI Qiong, MAO Wenwen, HU Jianbin. Identification and Low Temperature Stress Responsive Expression Analysis of TIFY Gene Family in Melon[J]. Acta Horticulturae Sinica, 2026, 53(5): 1343-1356.

图/表 8

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	袁晓, 蒋园园, 朱云娜, 曲姗姗, 王玉昆, 原远, 王斌. 2024. JAZ家族基因在采后黄瓜低温贮藏条件下的表达分析. 浙江农业学报, 36 (8):1820-1831. doi: 10.3969/j.issn.1004-1524.20231044
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基因 Gene	上游引物（5′-3′） Forward primer	下游引物（5′-3′） Reverse primer
CmTIFY1	CAGCAAAGCGTGGATTTGGTA	TCGAAACCGATTTAACGAGGC
CmTIFY4	TGCAGACTTCCAGTGACAGAG	CGACAATCCAGAATCCCGCA
CmTIFY5A	ACGTCGAGGAGAGATCGACA	TAGAGAGCCCAGAACCGGAA
CmTIFY5B	CCACGCCTGATCTTACCGAG	CCCGCTCCTCATCCATACTC
CmTIFY5C	TGAACTTCAGGCAAGAGCCAT	ATGGAGAAACCTGCGTTCCC
CmTIFY5D	GGCAACCAGAGAAATGGAGGA	TTTCTTCATCGAAAGCCCCG
CmTIFY6A	ATGTTCTTAGCTGGGGCTGG	ATAGGAGCAGCATCACTTGC
CmTIFY6B	TTGCAACCTCCCTTTCGGTT	CACACGGATCCGGCATAGAA
CmTIFY8A	AGCCACCACGAATTGCTTCT	ACACGAGGCACAAACTGACT
CmTIFY8B	GAAAGCTGTGTGTTGCTGGG	CTTACCACCACTGCTGCCAT
CmTIFY9A	GTTCTCTCGCCTTTGCCTCT	GAACGTCAAAAACGGCGACA
CmTIFY9B	CGCCTCTGAATCCAGGTTGA	TGCTTTTGGCCTCATCACGA
CmTIFY10	ACGCTGGCCAAGTTTTTGTG	GAGTTCCGACCAAGATGGGG
CmTIFY11A	AGAAATCTGCCTCTGTGGCG	CCCTCTCCGACGGTAGATCA
CmTIFY11B	CGCCGGAAAAGTTTTGGTGT	ATAATCTGTTGGGGGCGACG
CmActin	GCCCTTCCTCATGCCATTCT	CAATTTCCCGTTCGGCAGTG

基因 Gene	上游引物（5′-3′） Forward primer	下游引物（5′-3′） Reverse primer
CmTIFY1	CAGCAAAGCGTGGATTTGGTA	TCGAAACCGATTTAACGAGGC
CmTIFY4	TGCAGACTTCCAGTGACAGAG	CGACAATCCAGAATCCCGCA
CmTIFY5A	ACGTCGAGGAGAGATCGACA	TAGAGAGCCCAGAACCGGAA
CmTIFY5B	CCACGCCTGATCTTACCGAG	CCCGCTCCTCATCCATACTC
CmTIFY5C	TGAACTTCAGGCAAGAGCCAT	ATGGAGAAACCTGCGTTCCC
CmTIFY5D	GGCAACCAGAGAAATGGAGGA	TTTCTTCATCGAAAGCCCCG
CmTIFY6A	ATGTTCTTAGCTGGGGCTGG	ATAGGAGCAGCATCACTTGC
CmTIFY6B	TTGCAACCTCCCTTTCGGTT	CACACGGATCCGGCATAGAA
CmTIFY8A	AGCCACCACGAATTGCTTCT	ACACGAGGCACAAACTGACT
CmTIFY8B	GAAAGCTGTGTGTTGCTGGG	CTTACCACCACTGCTGCCAT
CmTIFY9A	GTTCTCTCGCCTTTGCCTCT	GAACGTCAAAAACGGCGACA
CmTIFY9B	CGCCTCTGAATCCAGGTTGA	TGCTTTTGGCCTCATCACGA
CmTIFY10	ACGCTGGCCAAGTTTTTGTG	GAGTTCCGACCAAGATGGGG
CmTIFY11A	AGAAATCTGCCTCTGTGGCG	CCCTCTCCGACGGTAGATCA
CmTIFY11B	CGCCGGAAAAGTTTTGGTGT	ATAATCTGTTGGGGGCGACG
CmActin	GCCCTTCCTCATGCCATTCT	CAATTTCCCGTTCGGCAGTG

基因 Gene	基因序列号 Gene ID	CDS长度/bp CDS length	氨基酸数 Number of amino acids	相对分子质量/kD Molecular weight	等电点 pI	亚细胞定位 Subcellular localization
CmTIFY1	MELO3C011079	882	293	31.69	6.60	细胞核Nucleus
CmTIFY4	MELO3C014283	921	306	33.33	5.39	细胞核Nucleus
CmTIFY5A	MELO3C001202	450	149	16.83	9.02	细胞核Nucleus
CmTIFY5B	MELO3C002741	363	120	13.44	10.03	细胞核Nucleus
CmTIFY5C	MELO3C016195	384	127	14.84	10.88	细胞核Nucleus
CmTIFY5D	MELO3C007125	399	132	14.67	10.23	细胞核Nucleus
CmTIFY6A	MELO3C015030	1 062	353	36.71	8.66	细胞核Nucleus
CmTIFY6B	MELO3C002143	1 032	343	36.83	9.12	细胞核Nucleus
CmTIFY8A	MELO3C003675	1 215	404	43.32	8.61	细胞核Nucleus
CmTIFY8B	MELO3C011217	1 362	453	47.69	9.05	细胞核Nucleus
CmTIFY9A	MELO3C022348	558	185	20.35	9.64	细胞核Nucleus
CmTIFY9B	MELO3C017797	603	200	22.23	7.95	细胞核Nucleus
CmTIFY10	MELO3C015538	690	229	24.61	10.06	细胞核Nucleus
CmTIFY11A	MELO3C022678	630	209	22.73	9.32	细胞核Nucleus
CmTIFY11B	MELO3C024336	903	300	32.46	9.38	细胞核Nucleus

基因 Gene	基因序列号 Gene ID	CDS长度/bp CDS length	氨基酸数 Number of amino acids	相对分子质量/kD Molecular weight	等电点 pI	亚细胞定位 Subcellular localization
CmTIFY1	MELO3C011079	882	293	31.69	6.60	细胞核Nucleus
CmTIFY4	MELO3C014283	921	306	33.33	5.39	细胞核Nucleus
CmTIFY5A	MELO3C001202	450	149	16.83	9.02	细胞核Nucleus
CmTIFY5B	MELO3C002741	363	120	13.44	10.03	细胞核Nucleus
CmTIFY5C	MELO3C016195	384	127	14.84	10.88	细胞核Nucleus
CmTIFY5D	MELO3C007125	399	132	14.67	10.23	细胞核Nucleus
CmTIFY6A	MELO3C015030	1 062	353	36.71	8.66	细胞核Nucleus
CmTIFY6B	MELO3C002143	1 032	343	36.83	9.12	细胞核Nucleus
CmTIFY8A	MELO3C003675	1 215	404	43.32	8.61	细胞核Nucleus
CmTIFY8B	MELO3C011217	1 362	453	47.69	9.05	细胞核Nucleus
CmTIFY9A	MELO3C022348	558	185	20.35	9.64	细胞核Nucleus
CmTIFY9B	MELO3C017797	603	200	22.23	7.95	细胞核Nucleus
CmTIFY10	MELO3C015538	690	229	24.61	10.06	细胞核Nucleus
CmTIFY11A	MELO3C022678	630	209	22.73	9.32	细胞核Nucleus
CmTIFY11B	MELO3C024336	903	300	32.46	9.38	细胞核Nucleus

甜瓜TIFY基因家族成员鉴定及低温胁迫响应表达分析

Identification and Low Temperature Stress Responsive Expression Analysis of TIFY Gene Family in Melon

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甜瓜TIFY基因家族成员鉴定及低温胁迫响应表达分析

Identification and Low Temperature Stress Responsive Expression Analysis of TIFY Gene Family in Melon

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