杏果实中调控有机酸积累的QTL定位及其主效基因筛选

doi:10.16420/j.issn.0513-353x.2024-0438

园艺学报 ›› 2025, Vol. 52 ›› Issue (4): 846-856.doi: 10.16420/j.issn.0513-353x.2024-0438

• 遗传育种·种质资源·分子生物学 • 上一篇下一篇

杏果实中调控有机酸积累的QTL定位及其主效基因筛选

姜凤超，杨丽，张俊环，张美玲，于文剑，孙浩元^*(zl249@ibcas.ac.cn)

北京市农林科学院林业果树研究所，农业农村部华北地区园艺作物生物学与种质创制重点实验室，农业农村部华北都市农业重点实验室，北京市落叶果树工程技术研究中心，国家林业与草原杏工程技术研究中心，北京 100093

收稿日期:2024-12-17 修回日期:2025-02-09 出版日期:2025-04-25 发布日期:2025-04-25
通讯作者: ^*E-mail：zl249@ibcas.ac.cn
基金资助:
国家自然科学基金项目（32025032，32441067）

QTL Mapping and Identification of Major Genes Regulating Organic Acid Accumulation in Apricot Fruits

JIANG Fengchao，YANG Li，ZHANG Junhuan，ZHANG Meiling，YU Wenjian，and SUN Haoyuan*(zl249@ibcas.ac.cn)

Institute of Forestry and Pomology，Beijing Academy of Agriculture and Forestry Sciences/Key Laboratory of Biology and Genetic Improvement of Horticultural Crops（North China），Ministry of Agriculture and Rural Affairs/Key Laboratory of Urban Agriculture（North China），Ministry of Agriculture and Rural Affairs/Beijing Engineering Research Center for Deciduous Fruit Trees/Apricot Engineering and Technology Research Center of the National Forestry and Grassland Administration，Beijing 100093，China

Received:2024-12-17 Revised:2025-02-09 Published:2025-04-25 Online:2025-04-25

摘要/Abstract

摘要： 以‘串枝红’为母本和‘骆驼黄’为父本构建杏F₁杂交群体，并采用液相色谱测定果实中苹果酸、柠檬酸和总酸含量，同时，利用重测序数据挖掘杂交群体的SNP变异信息，构建杏遗传连锁图谱。结果表明，苹果酸在发育过程中先升高后降低，而柠檬酸呈出一直升高的变化规律。苹果酸和总酸具有典型的数量遗传特征，而柠檬酸呈现偏正态分布，可能存在主效调控基因。杏遗传连锁图谱标记平均距离为0.52 cM，利用该图谱定位获得11个QTL，其中，与苹果酸相关区间包含88个基因，与柠檬酸相关包含310个基因，与总酸相关包含40个基因。加权基因共表达网络分析（WGCNA）表明苹果酸、柠檬酸和总酸分别与模块m13、m05和m08显著相关，富集分析发现柠檬酸相关候选基因主要与三羧酸循环循环（TCA）、丙酮酸代谢、糖酵解/糖原异生等途径和通路相关。对QTL、WGCNA和TCA循环涉及的基因取交集，发现仅PA08G28879在三者中同时出现，其可能是调控杏果实中柠檬酸积累的主效基因（PaMDH1），可作为探索杏果实有机酸积累分子机制的候选基因。

关键词: 杏, 杂交群体, 有机酸, 遗传变异, QTL定位

Abstract:

Based on a cross between Chuanzhihong（female）and Luotuohuang（male），an F₁ hybrid population of apricot was constructed. The levels of malic acid，citric acid，and total acids in the fruits were determined using liquid chromatography. Additionally，SNP variations in the hybrid population were identified through resequencing data to construct a genetic linkage map for apricots. The findings revealed that malic acid levels increased initially and then decreased during fruit development，while citric acid levels showed a continuous increase. Malic acid and total acids displayed typical quantitative genetic characteristics，whereas citric acid exhibited a positively skewed distribution，indicating the presence of major regulatory genes. The average inter-marker distance in the apricot genetic linkage map was 0.52 cM. Using this map，a total of 11 QTLs were identified，with 88 genes associated with malic acid，310 genes associated with citric acid，and 40 genes associated with total acids. WGCNA analysis demonstrated significant correlations between malic acid，citric acid，total acids，and modules m13，m05，and m08，respectively. Enrichment analysis revealed that candidate genes associated with citric acid were primarily involved in the TCA cycle，pyruvate metabolism，glycolysis/gluconeogenesis，and other related pathways. Intersection analysis of genes related to QTLs，WGCNA，and the TCA cycle identified a single gene，PA08G28879（PaMDH1），present in all three analyses，which may serve as the major gene regulating citric acid accumulation and the candidate gene for exploring the molecular mechanism of organic acid in apricot fruits.

Key words: apricot, F₁ population, organic acids, genetic variation, QTL mapping

中图分类号:

S 662.2

姜凤超, 杨丽, 张俊环, 张美玲, 于文剑, 孙浩元. 杏果实中调控有机酸积累的QTL定位及其主效基因筛选[J]. 园艺学报, 2025, 52(4): 846-856.

JIANG Fengchao, YANG Li, ZHANG Junhuan, ZHANG Meiling, YU Wenjian, SUN Haoyuan. QTL Mapping and Identification of Major Genes Regulating Organic Acid Accumulation in Apricot Fruits[J]. Acta Horticulturae Sinica, 2025, 52(4): 846-856.

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杏果实中调控有机酸积累的QTL定位及其主效基因筛选

QTL Mapping and Identification of Major Genes Regulating Organic Acid Accumulation in Apricot Fruits

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杏果实中调控有机酸积累的QTL定位及其主效基因筛选

QTL Mapping and Identification of Major Genes Regulating Organic Acid Accumulation in Apricot Fruits

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