蓝莓C2H2家族基因在花芽休眠解除中的作用初探

doi:10.16420/j.issn.0513-353x.2022-0044

摘要/Abstract

摘要：

为了解蓝莓C₂H₂锌指蛋白在花芽休眠中的功能，通过生物信息鉴定蓝莓C₂H₂-ZFP基因家族成员，利用转录组数据和荧光定量PCR分析其在花芽内休眠解除过程和外源脱落酸处理下的表达模式。在蓝莓中共鉴定到VcC₂H₂-ZFP家族79个成员，通过系统进化树和保守基序将其分为3组。分析表明：VcC₂H₂-ZFP家族成员在蓝莓各器官中均有表达，其中15个在花芽内休眠解除过程中表达存在显著差异，如Vc31g303.5、Vc7g51.6、Vc16g355.5在深度内休眠时期表达量高，而休眠解除后表达量较低。VcC₂H₂-ZFP启动子含有大量光反应、防御应激、脱落酸、赤霉素等激素响应元件。外源脱落酸处理花芽能显著促进Vc16g355.5、Vc7g51.6、Vc31g303.5等的表达。本研究结果揭示蓝莓部分VcC₂H₂-ZFP可能通过响应脱落酸参与花芽休眠过程的调控。

关键词: 蓝莓, 花芽, 休眠, C₂H₂家族基因, 脱落酸

Abstract:

In order to explore the function of C₂H₂ zinc finger protein in blueberry flower bud dormancy，members of VcC₂H₂-ZFP gene family were identified in blueberry by bioinformatics analysis. The expression pattern of C₂H₂ genes during the process of flower bud endodormancy release and treatment of exogenous abscisic acid（ABA）were analyzed through transcriptome data and Quantitative Real-time PCR（qRT-PCR）. In this study，79 VcC₂H₂-ZFP family members were identified in blueberry. According to the results of phylogenetic tree and conservative motif analysis，VcC₂H₂-ZFP family members were divided into three groups. The results of gene expression showed that VcC₂H₂-ZFP genes family were expressed in all organs of blueberry，among which 15 VcC₂H₂-ZFP genes were significantly differentially expressed during flower bud endodormancy release；the expression levels of Vc31g303.5，Vc7g51.6，and Vc16g355.5 were high at deep endodormancy stage，but decreased with the process of dormancy release. Cis-acting element analysis showed that the promoter of VcC₂H₂-ZFP genes contained a large number of hormone response elements such as photoreaction，stress defense，ABA，and gibberellin. The results of qRT-PCR showed that exogenous ABA treatment of blueberry flower buds can significantly promote the expression of VcC₂H₂-ZFP genes such as Vc16g355.5，Vc7g51.6，and Vc31g303.5. The results revealed that part of VcC₂H₂-ZFP might participate in the regulation of blueberry flower bud dormancy by responding to ABA.

Key words: blueberry, flower bud, dormancy, C₂H₂family gene, abscisic acid

中图分类号:

S663.2

李可, 申朦晓, 潘炜浩, 章诗渲, 毛欣烨, 尹亚红, 李永强, 朱友银, 郭卫东. 蓝莓C₂H₂家族基因在花芽休眠解除中的作用初探[J]. 园艺学报, 2023, 50(4): 737-753.

LI Ke, SHEN Mengxiao, PAN Weihao, ZHANG Shixuan, MAO Xinye, YIN Yahong, LI Yongqiang, ZHU Youyin, GUO Weidong. Preliminary Investigation of C₂H₂ Family Genes in Blueberry Flower Bud Dormancy Release[J]. Acta Horticulturae Sinica, 2023, 50(4): 737-753.

图/表 10

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目的基因 Gene name	上游引物（5′-3′） Forward primer	下游引物（5′-3′） Reverse primer	扩增长度/bp Amplicon size	退火温度/℃ T_m
Vc38g176.32	TTATTCGGTGGTTCACGGCA	TTGCGAAGTCACGGATGGAA	365	55
Vc7g51.6	GCTCGATCTGTCACAAGAGTT	GACGTCGTAAGTCCACTGTTT	109	55
Vc3g10.6	AACAGAGGATTCGACCTGAAC	CCCAAATCAGGTTGGAAAGATAAA	155	55
Vc31g303.5	GGAGGAGCAAACAGTGGAATTA	CAAATCCGAATTCCGGCAAAG	112	55
Vc24g106.5	AAGAAGACGAAGAAGGAGGTAATAAG	GCGACACCGATTTGGTAGAA	192	55
Vc16g355.5	GGAGGAGCAAACAGTGGAATTA	CCAAATCCATATTCCGGCAAAG	113	55
Vc14g348.9	GTGTGACGACGACATCTGAA	CCCAAATCAGGTTGGAAAGATAAA	196	55
Vc11g294.5	CGATATGTGGGTCGGAGTTT	CATCGGTGCTGGTAGATTCA	183	55
Vc21g155.6	CAAAGGGCCCAACTGCAACAGAT	TACACCCCGAGAAGGGACACGAA	483	55
Vc23g300.2	CCACAGGTGCTTTCCAACGGGTCA	CGCCGAATCCTTGCCAGAATTCAG	194	60.5
Vc37g104.16	CTGATAAAATGGAGACTGATGAAA	CCAGACAACAGATAAGAAAACGAA	244	60.5
Vc37g230.20	TCGACAAAGGTGGTTCAGGT	TGTCCCTGTACCCATACCCA	213	60.5
Vc38g80.29	GAAGAGGAGAAAGGGAATGCCACT	AACCCATCAAGCAATCCATCAAAA	152	60.5
Vc42g36.8	GCAAAGGGCCCAACTGCAACAGAT	CACCCCGAGAAGGGACACGAAAAC	482	60.5
Vc6g338.31	GAAGAGGAGAAAGGGAATGCCACT	AACCCATCAAGCAATCCATCAAAA	152	62
VcGAPDH	TGAGAAAGAATACAAGCCAGAT	CAGGCAACACCTTACCAA	260	55 ~ 62

目的基因 Gene name	上游引物（5′-3′） Forward primer	下游引物（5′-3′） Reverse primer	扩增长度/bp Amplicon size	退火温度/℃ T_m
Vc38g176.32	TTATTCGGTGGTTCACGGCA	TTGCGAAGTCACGGATGGAA	365	55
Vc7g51.6	GCTCGATCTGTCACAAGAGTT	GACGTCGTAAGTCCACTGTTT	109	55
Vc3g10.6	AACAGAGGATTCGACCTGAAC	CCCAAATCAGGTTGGAAAGATAAA	155	55
Vc31g303.5	GGAGGAGCAAACAGTGGAATTA	CAAATCCGAATTCCGGCAAAG	112	55
Vc24g106.5	AAGAAGACGAAGAAGGAGGTAATAAG	GCGACACCGATTTGGTAGAA	192	55
Vc16g355.5	GGAGGAGCAAACAGTGGAATTA	CCAAATCCATATTCCGGCAAAG	113	55
Vc14g348.9	GTGTGACGACGACATCTGAA	CCCAAATCAGGTTGGAAAGATAAA	196	55
Vc11g294.5	CGATATGTGGGTCGGAGTTT	CATCGGTGCTGGTAGATTCA	183	55
Vc21g155.6	CAAAGGGCCCAACTGCAACAGAT	TACACCCCGAGAAGGGACACGAA	483	55
Vc23g300.2	CCACAGGTGCTTTCCAACGGGTCA	CGCCGAATCCTTGCCAGAATTCAG	194	60.5
Vc37g104.16	CTGATAAAATGGAGACTGATGAAA	CCAGACAACAGATAAGAAAACGAA	244	60.5
Vc37g230.20	TCGACAAAGGTGGTTCAGGT	TGTCCCTGTACCCATACCCA	213	60.5
Vc38g80.29	GAAGAGGAGAAAGGGAATGCCACT	AACCCATCAAGCAATCCATCAAAA	152	60.5
Vc42g36.8	GCAAAGGGCCCAACTGCAACAGAT	CACCCCGAGAAGGGACACGAAAAC	482	60.5
Vc6g338.31	GAAGAGGAGAAAGGGAATGCCACT	AACCCATCAAGCAATCCATCAAAA	152	62
VcGAPDH	TGAGAAAGAATACAAGCCAGAT	CAGGCAACACCTTACCAA	260	55 ~ 62

基因名 Gene name	基因ID Accession No.	氨基酸数 Amino acid number	分子量/kD Molecular weight	等电点 pI	不稳定系数 Instability index	亲水值 Hydropathicity	亚细胞定位 Subcellular location
Vc19g325.6	VaccDscaff19-processed-325.6	396	43.8	8.94	49.42	-0.696	Nucl，Cyto
Vc15g278.5	VaccDscaff15-processed-278.5	394	43.7	9.00	46.09	-0.704	Nucl，Cyto
Vc11g294.4	VaccDscaff11-processed-294.4	398	44.2	9.01	48.83	-0.727	Nucl，Cyto
Vc24g106.6	VaccDscaff24-processed-106.6	398	44.2	8.94	48.52	-0.734	Nucl，Cyto
Vc30g269.43	VaccDscaff30-augustus-269.43	508	56.4	6.79	41.71	-0.436	Nucl，Chlo
Vc13g13.36	VaccDscaff13-augustus-13.36	456	50.7	5.87	41.61	-0.398	Nucl
Vc42g47.29	VaccDscaff42-augustus-47.29	456	50.6	5.87	41.10	-0.397	Nucl
Vc47g65.34	VaccDscaff47-augustus-65.34	388	42.9	8.51	45.21	-0.701	Nucl
Vc43g136.13	VaccDscaff43-augustus-136.13	379	41.9	8.61	46.09	-0.635	Nucl
Vc22g249.38	VaccDscaff22-augustus-249.38	378	41.7	8.61	43.32	-0.599	Nucl
Vc39g85.33	VaccDscaff39-augustus-85.33	381	42.3	5.60	44.46	-0.452	Nucl
Vc37g230.20	VaccDscaff37-augustus-230.20	392	43.3	5.58	42.06	-0.421	Nucl
Vc38g80.29	VaccDscaff38-snap-80.29	392	43.1	5.44	43.73	-0.430	Nucl
Vc6g338.31	VaccDscaff6-augustus-338.31	392	43.2	5.43	42.87	-0.438	Nucl，Pero
Vc38g176.32	VaccDscaff38-augustus-176.32	478	53.4	5.84	50.44	-0.775	Nucl，Pero
Vc6g193.34	VaccDscaff6-augustus-193.34	478	53.4	5.84	50.44	-0.775	Nucl，Pero
Vc37g104.16	VaccDscaff37-augustus-104.16	478	53.4	5.84	50.88	-0.756	Nucl，Pero
Vc32g62.9	VaccDscaff32-processed-62.9	382	41.4	6.57	53.67	-0.656	Nucl
Vc32g14.2	VaccDscaff32-processed-14.2	474	52.0	8.39	54.84	-0.510	Nucl
Vc13g317.8	VaccDscaff13-processed-317.8	477	52.1	8.09	55.80	-0.521	Nucl
Vc30g79.13	VaccDscaff30-processed-79.13	474	52.0	8.07	54.80	-0.524	Nucl
Vc19g325.7	VaccDscaff19-processed-325.7	254	27.9	5.94	64.39	-0.774	Nucl
Vc15g278.6	VaccDscaff15-processed-278.6	254	27.9	5.94	64.05	-0.784	Nucl
Vc11g294.5	VaccDscaff11-processed-294.5	254	28.0	5.81	65.34	-0.805	Nucl
Vc24g106.5	VaccDscaff24-processed-106.5	254	28.0	5.88	63.53	-0.826	Nucl
Vc33g157.2	VaccDscaff33-processed-157.2	244	27.5	7.11	74.79	-0.976	Nucl
Vc21g197.4	VaccDscaff21-processed-197.4	245	27.6	7.11	73.79	-0.938	Nucl
Vc29g187.1	VaccDscaff29-processed-187.1	245	27.6	7.11	74.52	-0.953	Nucl
Vc19g69.12	VaccDscaff19-processed-69.12	355	39.0	5.51	69.83	-0.699	Nucl
Vc26g194.1	VaccDscaff26-processed-194.1	244	27.5	6.51	76.04	-0.933	Nucl
Vc20g74.12	VaccDscaff20-processed-74.12	355	38.8	5.51	68.93	-0.683	Nucl
Vc28g285.19	VaccDscaff28-processed-285.19	355	38.9	5.49	68.69	-0.692	Nucl
Vc21g236.10	VaccDscaff21-processed-236.10	286	31.0	7.65	56.50	-0.810	Nucl
Vc26g232.8	VaccDscaff26-processed-232.8	286	31.1	8.14	54.76	-0.819	Nucl
Vc33g119.6	VaccDscaff33-processed-119.6	287	31.1	7.65	54.81	-0.808	Nucl
Vc40g163.1	VaccDscaff40-processed-163.1	371	41.9	5.75	64.69	-1.037	Nucl
Vc41g152.1	VaccDscaff41-processed-152.1	373	42.1	5.82	62.04	-1.050	Nucl
Vc23g116.9	VaccDscaff23-processed-116.9	373	42.1	5.68	61.92	-1.053	Nucl
Vc23g130.8	VaccDscaff23-processed-130.8	371	41.8	5.81	64.61	-1.011	Nucl
Vc29g224.1	VaccDscaff29-processed-224.1	275	29.7	8.45	53.45	-0.817	Nucl，Extr
Vc47g65.8	VaccDscaff47-processed-65.8	275	29.9	6.32	56.61	-0.635	Nucl
Vc18g40.7	VaccDscaff18-processed-40.7	236	24.6	8.93	59.17	-0.567	Nucl
Vc43g135.1	VaccDscaff43-processed-135.1	272	29.6	6.25	55.65	-0.584	Nucl
Vc16g355.5	VaccDscaff16-processed-355.5	234	24.4	8.92	59.76	-0.562	Nucl
Vc22g250.7	VaccDscaff22-processed-250.7	272	29.6	6.25	55.53	-0.606	Nucl
Vc7g51.6	VaccDscaff7-processed-51.6	224	23.5	9.01	62.98	-0.544	Nucl
Vc31g303.5	VaccDscaff31-processed-303.5	233	24.3	8.93	61.63	-0.561	Nucl
Vc40g40.5	VaccDscaff40-processed-40.5	240	25.5	7.72	71.73	-0.707	Nucl
Vc23g300.2	VaccDscaff23-processed-300.2	239	25.4	8.22	71.35	-0.700	Nucl
Vc12g112.2	VaccDscaff12-processed-112.2	240	25.5	8.17	70.40	-0.668	Nucl
Vc1g306.32	VaccDscaff1-processed-306.32	239	25.8	7.87	57.87	-0.782	Nucl
Vc10g124.14	VaccDscaff10-processed-124.14	306	33.2	7.86	64.71	-0.660	Nucl
Vc3g10.6	VaccDscaff3-processed-10.6	217	23.4	8.68	67.52	-0.675	Nucl
Vc14g348.9	VaccDscaff14-processed-348.9	233	24.7	8.67	61.00	-0.624	Nucl
Vc2g402.7	VaccDscaff2-processed-402.7	234	24.8	8.67	59.96	-0.573	Nucl
Vc22g45.12	VaccDscaff22-processed-45.12	371	42.3	6.26	58.05	-1.124	Nucl
Vc21g155.6	VaccDscaff21-processed-155.6	182	20.4	8.84	51.53	-0.348	Nucl
Vc26g158.3	VaccDscaff26-processed-158.3	182	20.4	8.67	52.00	-0.348	Nucl
Vc33g183.0	VaccDscaff33-processed-183.0	182	20.4	8.67	52.00	-0.348	Nucl
Vc29g155.8	VaccDscaff29-processed-155.8	182	20.4	8.66	55.59	-0.415	Nucl
Vc20g57.10	VaccDscaff20-processed-57.10	161	17.7	9.02	53.48	-0.437	Nucl，Cyto，Extr
Vc48g64.13	VaccDscaff48-processed-64.13	173	18.9	9.34	48.58	-0.395	Nucl，Cyto，Extr
Vc14g51.11	VaccDscaff14-processed-51.11	228	25.1	5.19	64.33	-0.483	Nucl，Pero
Vc20g133.0	VaccDscaff20-processed-133.0	228	24.2	7.08	60.52	-0.638	Nucl
Vc28g213.2	VaccDscaff28-processed-213.2	228	24.2	7.08	60.52	-0.638	Nucl
Vc2g14.22	VaccDscaff2-processed-14.22	228	25.2	5.07	61.95	-0.466	Nucl，Pero
Vc42g36.8	VaccDscaff42-processed-36.8	232	25.4	5.16	46.05	-0.316	Nucl，Pero
Vc19g135.9	VaccDscaff19-processed-135.9	222	23.7	7.08	55.23	-0.605	Nucl，Pero
Vc30g280.2	VaccDscaff30-processed-280.2	232	25.5	5.16	46.41	-0.319	Nucl
Vc27g295.0	VaccDscaff27-processed-295.0	263	28.8	8.80	62.35	-0.655	Nucl，Pero
Vc17g324.14	VaccDscaff17-processed-324.14	263	28.8	8.59	62.70	-0.664	Nucl，Pero
Vc34g49.12	VaccDscaff34-processed-49.12	263	28.8	8.59	62.70	-0.664	Nucl，Pero
Vc44g170.19	VaccDscaff44-augustus-170.19	248	27.0	7.14	60.50	-0.597	Nucl
Vc20g156.15	VaccDscaff20-augustus-156.15	248	27.0	6.50	57.33	-0.583	Nucl
Vc28g167.32	VaccDscaff28-augustus-167.32	248	27.0	6.75	60.99	-0.583	Nucl
Vc44g65.1	VaccDscaff44-processed-65.1	247	26.6	8.38	54.41	-0.681	Nucl
Vc28g73.2	VaccDscaff28-processed-73.2	247	26.6	8.38	53.87	-0.681	Nucl
Vc20g302.3	VaccDscaff20-processed-302.3	250	26.9	7.80	55.74	-0.688	Nucl
Vc8g145.9	VaccDscaff8-processed-145.9	237	25.8	7.87	57.57	-0.784	Nucl

基因名 Gene name	基因ID Accession No.	氨基酸数 Amino acid number	分子量/kD Molecular weight	等电点 pI	不稳定系数 Instability index	亲水值 Hydropathicity	亚细胞定位 Subcellular location
Vc19g325.6	VaccDscaff19-processed-325.6	396	43.8	8.94	49.42	-0.696	Nucl，Cyto
Vc15g278.5	VaccDscaff15-processed-278.5	394	43.7	9.00	46.09	-0.704	Nucl，Cyto
Vc11g294.4	VaccDscaff11-processed-294.4	398	44.2	9.01	48.83	-0.727	Nucl，Cyto
Vc24g106.6	VaccDscaff24-processed-106.6	398	44.2	8.94	48.52	-0.734	Nucl，Cyto
Vc30g269.43	VaccDscaff30-augustus-269.43	508	56.4	6.79	41.71	-0.436	Nucl，Chlo
Vc13g13.36	VaccDscaff13-augustus-13.36	456	50.7	5.87	41.61	-0.398	Nucl
Vc42g47.29	VaccDscaff42-augustus-47.29	456	50.6	5.87	41.10	-0.397	Nucl
Vc47g65.34	VaccDscaff47-augustus-65.34	388	42.9	8.51	45.21	-0.701	Nucl
Vc43g136.13	VaccDscaff43-augustus-136.13	379	41.9	8.61	46.09	-0.635	Nucl
Vc22g249.38	VaccDscaff22-augustus-249.38	378	41.7	8.61	43.32	-0.599	Nucl
Vc39g85.33	VaccDscaff39-augustus-85.33	381	42.3	5.60	44.46	-0.452	Nucl
Vc37g230.20	VaccDscaff37-augustus-230.20	392	43.3	5.58	42.06	-0.421	Nucl
Vc38g80.29	VaccDscaff38-snap-80.29	392	43.1	5.44	43.73	-0.430	Nucl
Vc6g338.31	VaccDscaff6-augustus-338.31	392	43.2	5.43	42.87	-0.438	Nucl，Pero
Vc38g176.32	VaccDscaff38-augustus-176.32	478	53.4	5.84	50.44	-0.775	Nucl，Pero
Vc6g193.34	VaccDscaff6-augustus-193.34	478	53.4	5.84	50.44	-0.775	Nucl，Pero
Vc37g104.16	VaccDscaff37-augustus-104.16	478	53.4	5.84	50.88	-0.756	Nucl，Pero
Vc32g62.9	VaccDscaff32-processed-62.9	382	41.4	6.57	53.67	-0.656	Nucl
Vc32g14.2	VaccDscaff32-processed-14.2	474	52.0	8.39	54.84	-0.510	Nucl
Vc13g317.8	VaccDscaff13-processed-317.8	477	52.1	8.09	55.80	-0.521	Nucl
Vc30g79.13	VaccDscaff30-processed-79.13	474	52.0	8.07	54.80	-0.524	Nucl
Vc19g325.7	VaccDscaff19-processed-325.7	254	27.9	5.94	64.39	-0.774	Nucl
Vc15g278.6	VaccDscaff15-processed-278.6	254	27.9	5.94	64.05	-0.784	Nucl
Vc11g294.5	VaccDscaff11-processed-294.5	254	28.0	5.81	65.34	-0.805	Nucl
Vc24g106.5	VaccDscaff24-processed-106.5	254	28.0	5.88	63.53	-0.826	Nucl
Vc33g157.2	VaccDscaff33-processed-157.2	244	27.5	7.11	74.79	-0.976	Nucl
Vc21g197.4	VaccDscaff21-processed-197.4	245	27.6	7.11	73.79	-0.938	Nucl
Vc29g187.1	VaccDscaff29-processed-187.1	245	27.6	7.11	74.52	-0.953	Nucl
Vc19g69.12	VaccDscaff19-processed-69.12	355	39.0	5.51	69.83	-0.699	Nucl
Vc26g194.1	VaccDscaff26-processed-194.1	244	27.5	6.51	76.04	-0.933	Nucl
Vc20g74.12	VaccDscaff20-processed-74.12	355	38.8	5.51	68.93	-0.683	Nucl
Vc28g285.19	VaccDscaff28-processed-285.19	355	38.9	5.49	68.69	-0.692	Nucl
Vc21g236.10	VaccDscaff21-processed-236.10	286	31.0	7.65	56.50	-0.810	Nucl
Vc26g232.8	VaccDscaff26-processed-232.8	286	31.1	8.14	54.76	-0.819	Nucl
Vc33g119.6	VaccDscaff33-processed-119.6	287	31.1	7.65	54.81	-0.808	Nucl
Vc40g163.1	VaccDscaff40-processed-163.1	371	41.9	5.75	64.69	-1.037	Nucl
Vc41g152.1	VaccDscaff41-processed-152.1	373	42.1	5.82	62.04	-1.050	Nucl
Vc23g116.9	VaccDscaff23-processed-116.9	373	42.1	5.68	61.92	-1.053	Nucl
Vc23g130.8	VaccDscaff23-processed-130.8	371	41.8	5.81	64.61	-1.011	Nucl
Vc29g224.1	VaccDscaff29-processed-224.1	275	29.7	8.45	53.45	-0.817	Nucl，Extr
Vc47g65.8	VaccDscaff47-processed-65.8	275	29.9	6.32	56.61	-0.635	Nucl
Vc18g40.7	VaccDscaff18-processed-40.7	236	24.6	8.93	59.17	-0.567	Nucl
Vc43g135.1	VaccDscaff43-processed-135.1	272	29.6	6.25	55.65	-0.584	Nucl
Vc16g355.5	VaccDscaff16-processed-355.5	234	24.4	8.92	59.76	-0.562	Nucl
Vc22g250.7	VaccDscaff22-processed-250.7	272	29.6	6.25	55.53	-0.606	Nucl
Vc7g51.6	VaccDscaff7-processed-51.6	224	23.5	9.01	62.98	-0.544	Nucl
Vc31g303.5	VaccDscaff31-processed-303.5	233	24.3	8.93	61.63	-0.561	Nucl
Vc40g40.5	VaccDscaff40-processed-40.5	240	25.5	7.72	71.73	-0.707	Nucl
Vc23g300.2	VaccDscaff23-processed-300.2	239	25.4	8.22	71.35	-0.700	Nucl
Vc12g112.2	VaccDscaff12-processed-112.2	240	25.5	8.17	70.40	-0.668	Nucl
Vc1g306.32	VaccDscaff1-processed-306.32	239	25.8	7.87	57.87	-0.782	Nucl
Vc10g124.14	VaccDscaff10-processed-124.14	306	33.2	7.86	64.71	-0.660	Nucl
Vc3g10.6	VaccDscaff3-processed-10.6	217	23.4	8.68	67.52	-0.675	Nucl
Vc14g348.9	VaccDscaff14-processed-348.9	233	24.7	8.67	61.00	-0.624	Nucl
Vc2g402.7	VaccDscaff2-processed-402.7	234	24.8	8.67	59.96	-0.573	Nucl
Vc22g45.12	VaccDscaff22-processed-45.12	371	42.3	6.26	58.05	-1.124	Nucl
Vc21g155.6	VaccDscaff21-processed-155.6	182	20.4	8.84	51.53	-0.348	Nucl
Vc26g158.3	VaccDscaff26-processed-158.3	182	20.4	8.67	52.00	-0.348	Nucl
Vc33g183.0	VaccDscaff33-processed-183.0	182	20.4	8.67	52.00	-0.348	Nucl
Vc29g155.8	VaccDscaff29-processed-155.8	182	20.4	8.66	55.59	-0.415	Nucl
Vc20g57.10	VaccDscaff20-processed-57.10	161	17.7	9.02	53.48	-0.437	Nucl，Cyto，Extr
Vc48g64.13	VaccDscaff48-processed-64.13	173	18.9	9.34	48.58	-0.395	Nucl，Cyto，Extr
Vc14g51.11	VaccDscaff14-processed-51.11	228	25.1	5.19	64.33	-0.483	Nucl，Pero
Vc20g133.0	VaccDscaff20-processed-133.0	228	24.2	7.08	60.52	-0.638	Nucl
Vc28g213.2	VaccDscaff28-processed-213.2	228	24.2	7.08	60.52	-0.638	Nucl
Vc2g14.22	VaccDscaff2-processed-14.22	228	25.2	5.07	61.95	-0.466	Nucl，Pero
Vc42g36.8	VaccDscaff42-processed-36.8	232	25.4	5.16	46.05	-0.316	Nucl，Pero
Vc19g135.9	VaccDscaff19-processed-135.9	222	23.7	7.08	55.23	-0.605	Nucl，Pero
Vc30g280.2	VaccDscaff30-processed-280.2	232	25.5	5.16	46.41	-0.319	Nucl
Vc27g295.0	VaccDscaff27-processed-295.0	263	28.8	8.80	62.35	-0.655	Nucl，Pero
Vc17g324.14	VaccDscaff17-processed-324.14	263	28.8	8.59	62.70	-0.664	Nucl，Pero
Vc34g49.12	VaccDscaff34-processed-49.12	263	28.8	8.59	62.70	-0.664	Nucl，Pero
Vc44g170.19	VaccDscaff44-augustus-170.19	248	27.0	7.14	60.50	-0.597	Nucl
Vc20g156.15	VaccDscaff20-augustus-156.15	248	27.0	6.50	57.33	-0.583	Nucl
Vc28g167.32	VaccDscaff28-augustus-167.32	248	27.0	6.75	60.99	-0.583	Nucl
Vc44g65.1	VaccDscaff44-processed-65.1	247	26.6	8.38	54.41	-0.681	Nucl
Vc28g73.2	VaccDscaff28-processed-73.2	247	26.6	8.38	53.87	-0.681	Nucl
Vc20g302.3	VaccDscaff20-processed-302.3	250	26.9	7.80	55.74	-0.688	Nucl
Vc8g145.9	VaccDscaff8-processed-145.9	237	25.8	7.87	57.57	-0.784	Nucl

蓝莓C₂H₂家族基因在花芽休眠解除中的作用初探

Preliminary Investigation of C₂H₂ Family Genes in Blueberry Flower Bud Dormancy Release

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