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园艺学报 ›› 2021, Vol. 48 ›› Issue (1): 60-72.doi: 10.16420/j.issn.0513-353x.2020-0295

• 研究论文 • 上一篇    下一篇

菜薹BrNAP克隆及其在采后叶片衰老中的功能分析

李钰卓1, 刘柯1, 袁璐1, 曹丽雯1, 王挺进1, 甘苏生2,**(), 陈利萍1,**()   

  1. 1浙江大学农业与生物技术学院园艺系,杭州 310058
    2康奈尔大学植物科学学院植物生物学系,纽约州伊萨卡 14853
  • 收稿日期:2020-06-08 修回日期:2020-07-27 出版日期:2021-01-25 发布日期:2021-01-29
  • 通讯作者: 甘苏生,陈利萍 E-mail:sg288@cornell.edu;chenliping@zju.edu.cn
  • 基金资助:
    国家重点研发计划项目(2019YFD1000300)

Cloning and Functional Analyses of BrNAP in Postharvest Leaf Senescence in Chinese Flowering Cabbage

LI Yuzhuo1, LIU Ke1, YUAN Lu1, CAO Liwen1, WANG Tingjin1, GAN Susheng2,**(), CHEN Liping1,**()   

  1. 1Department of Horticulture,College of Agriculture and Biotechnology,Zhejiang University,Hangzhou 310058,China
    2Plant Biology Section,School of Integrative Plant Science,Cornell University,Ithaca,NY 14853,USA
  • Received:2020-06-08 Revised:2020-07-27 Online:2021-01-25 Published:2021-01-29
  • Contact: GAN Susheng,CHEN Liping E-mail:sg288@cornell.edu;chenliping@zju.edu.cn

摘要:

在菜薹(Brassica rapa var. parachinensis)中克隆BrNAP1并分析其功能。BrNAP1编码区全长813 bp,编码270个氨基酸,具有NAP转录因子特有的保守结构域,属于NAP亚家族成员。BrNAP1表达量与叶片衰老程度呈正相关且受ABA诱导表达上调。亚细胞定位试验表明BrNAP1定位于细胞核。互补试验显示BrNAP1能使拟南芥atnap滞绿表型回复至野生型,过表达则能引起采后叶片早衰。双荧光素酶试验表明BrNAP1能够激活BrSAG113表达。这些说明BrNAP1是菜薹采后叶片衰老的正调控基因。

关键词: 菜薹, 采后, 叶片衰老, BrNAP1, 功能分析, 脱落酸

Abstract:

This research revealed the cloning and functional analyses of AtNAP orthologous gene named BrNAP1 in Chinese flowering cabbage(Brassica rapa var. parachinensis). The coding region of BrNAP1 is 813 bp in length and encodes 270 amino acids. It has a unique conserved domain of NAP transcription factors and belongs to the NAP subfamily. BrNAP1 expression increases with the progression of leaf senescence and can be induced by ABA treatment. Subcellular localization experiment revealed that BrNAP1 is a nuclear protein. Complementary experiments showed that BrNAP1 can restore the Arabidopsis atnap phenotype to the wild type. Overexpression of BrNAP1 causes precocious leaf senescence. BrNAP1 transcriptionally induced the activity of BrSAG113 in dual-luciferase assay. These results suggested that BrNAP1 has the AtNAP-like function and is a positive regulator gene of leaf senescence in Chinese flowering cabbage.

Key words: Chinese flowering cabbage, postharvest, leaf senescence, BrNAP1, functional analysis, abscisic acid

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