园艺学报 ›› 2020, Vol. 47 ›› Issue (4): 613-622.doi: 10.16420/j.issn.0513-353x.2019-0548

• 研究论文 •    下一篇



  1. 山东农业大学园艺科学与工程学院,作物生物学国家重点试验室,山东果蔬优质高效生产协同创新中心,山东泰安 271018
  • 出版日期:2020-04-25 发布日期:2020-04-25
  • 基金资助:

Investigation on MdMYB2 from Apple in Response to Abiotic Stress

YANG Yuyiing,REN Yiran,ZHENG Pengfei,YOU Chunxiang,WANG Xiaofei*,and HAO Yujin*   

  1. College of Horticulture Science and Engineering,Shandong Agricultural University,State Key Laboratory of Crop Biology,Shandong Collaborative Innovation Center for Fruit and Vegetable Production with High Quality and Efficiency, Tai’an,Shandong 271018,China
  • Online:2020-04-25 Published:2020-04-25

摘要: 为阐明苹果(Malus × domestica)R2R3-MYB转录因子基因MdMYB2在非生物胁迫条件下的生物学功能,对MdMYB2的启动子序列进行分析,发现其含有非生物胁迫相关顺式作用元件,且MdMYB2的表达量受外源ABA、聚乙二醇(PEG)和4 ℃低温的诱导。在不同处理对MdMYB2过表达拟南芥植株和MdMYB2过表达苹果愈伤组织的试验中发现,外源ABA处理抑制了MdMYB2转基因拟南芥种子的萌发,且转基因幼苗提高了对ABA的敏感性、耐旱性和抗冷性。同时,过表达MdMYB2的苹果愈伤组织也表现出对ABA敏感、耐旱和抗冷的表型。以上结果说明MdMYB2在植物的逆境胁迫响应中发挥重要作用。

关键词: 苹果, MdMYB2, ABA, 干旱, 低温

Abstract: In order to elucidate the biological function of the R2R3-MYB transcription factor gene MdMYB2 of apple(Malus × domestica) under abiotic stress conditions,the promoter sequence of MdMYB2 was analyzed. It was found that the promoter sequence of MdMYB2 contained abscisic acid(ABA),defense,stress,and low-temperature related cis-acting elements,and the expression of MdMYB2 was regulated by exogenous ABA,polyethyleneglycol(PEG),and 4 ℃ treatments. For the MdMYB2-OE Arabidopsis plants and MdMYB2-OE apple calli,it was found that the application of ABA severely inhibited the germination of MdMYB2-OE Arabidopsis,while increased its ABA sensitivity,drought and cold tolerance. At the same time,MdMYB2-OE apple calli also showed sensitivity to exogenous ABA,as well as drought and cold tolerance. Overall,the above results indicated that MdMYB2 played an important role in the abiotic stress response of plants.

Key words: apple, MdMYB2, ABA, drought, low temperature