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园艺学报 ›› 2020, Vol. 47 ›› Issue (4): 653-664.doi: 10.16420/j.issn.0513-353x.2019-0474

• 研究论文 • 上一篇    下一篇

番茄AP2/ERF超家族重鉴定及过表达SlERF.D.3株系表型分析

徐志璇,任仲海*   

  1. 山东农业大学园艺科学与工程学院,山东果蔬优质高效生产协同创新中心/农业部黄淮地区园艺作物生物学与种质创制重点实验室,作物生物学国家重点实验室,山东泰安 271018
  • 出版日期:2020-04-25 发布日期:2020-04-25
  • 基金资助:
    山东省“双一流”专项建设项目(SYL2017YSTD06);山东省泰山学者建设工程项目(ts20130932)

Re-identification of Tomato AP2/ERF Transcription Factor Superfamily and Phenotypic Analysis of the Overexpressing SlERF.D.3 Lines

XU Zhixuan and REN Zhonghai*   

  1. State Key Laboratory of Crop Biology,Shandong Collaborative Innovation Center of Fruit & Vegetable Quality and Efficient Production,Key Laboratory of Biology and Genetic Improvement of Horticultural Crops in Huang-Huai Region,Ministry of Agriculture,College of Horticultural Science and Engineering,Shandong Agricultural University,Tai’an,Shandong 271018,China
  • Online:2020-04-25 Published:2020-04-25

摘要: 基于番茄基因组数据库的更新,系统地对番茄AP2/ERF超家族成员进行了更新,并对其进行了染色体定位、保守基序、基因结构和对灰霉菌及生长素胁迫响应的分析。共鉴定出141个ERF家族基因,其中新鉴定出的ERF亚家族成员42个;随机分布在12条染色体上,大都只含有外显子而无内含子,部分基因不同程度的受到灰霉菌和生长素处理的诱导表达。作为拟南芥AtERF109在番茄中的同源基因,SlERF.D.3的表达可被灰霉菌抑制且可迅速强烈地响应生长素处理。为进一步明确SlERF.D.3的生物学功能,以番茄‘Ailsa Craig’为材料克隆了此基因的编码区,构建过表达载体并转化番茄,获得了3个T0代过表达株系。与野生型相比,转基因株系的叶片较细长、向下卷曲,且果实出现乳状突起。这为研究SlERF.D.3在调节番茄的生长发育和胁迫响应过程中的作用奠定了基础。

关键词: 番茄, ERF转录因子家族, SlERF.D.3, 生长发育

Abstract: In this study,based on the update of the tomato genome database,we performed the systematical analysis of tomato AP2/ERF superfamily members,including their chromosomal localization,conserved motifs,gene structure,and responses to Botrytis cinerea and auxin stress. A total of 141 ERF family genes including 42 new ones were identified and randomly distributed on 12 chromosomes. Most of them only contained one exon. Some genes could be induced by B. cinerea and auxin treatment in different degrees. AtERF109 plays important roles in both growth and development and stress responses in Arabidopsis,and tomato homologous gene SlERF.D.3 could be inhibited by B. cinerea and induced by auxin rapidly and strongly. In order to further clarify the biological function of SlERF.D.3,its coding region of 888 bp was cloned from tomato variety‘Ailsa Craig’and constructed into the overexpression vector. Three transgenic tomato lines overexpressing SlERF.D.3 were obtained through Agrobacterium-mediated transformation and had more slender and downward curling leaves,and the external surface of the fruits had a small number of protuberances compared to wild type. This study provides a basis for further clarifying SlERF.D.3’s role in the regulation of plant growth and development and stress responses.

Key words: tomato, ERF family, SlERF.D.3, growth and development

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