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园艺学报 ›› 2019, Vol. 46 ›› Issue (8): 1553-1564.doi: 10.16420/j.issn.0513-353x.2018-1056

• 研究论文 • 上一篇    下一篇

菊花脑 × 甘菊种间F1杂种的鉴定和遗传多样性分析

刘颖鑫,李沛曈,迟天华,王海滨,管志勇,房伟民,陈发棣,张 飞*   

  1. 南京农业大学园艺学院,作物遗传与种质创新国家重点实验室,农业农村部景观设计重点实验室,南京 210095
  • 出版日期:2019-08-25 发布日期:2019-08-25
  • 基金资助:

    国家自然科学基金项目(31870306,31572152);江苏省优势学科建设工程项目

Identification and Genetic Diversity Analysis of Interspecific F1 Hybrids Between Chrysanthemum nankingense × C. lavandulifolium

LIU Yingxin,LI Peitong,CHI Tianhua,WANG Haibin,GUAN Zhiyong,FANG Weimin,CHEN Fadi,and ZHANG Fei*   

  1. State Key Laboratory of Crop Genetics and Germplasm Enhancement,Key Laboratory of Landscaping,Ministry of Agriculture and Rural Affairs,College of Horticulture,Nanjing Agricultural University,Nanjing 210095,China
  • Online:2019-08-25 Published:2019-08-25

摘要:

利用SSR和SRAP标记及表型性状研究了二倍体菊花近缘种菊花脑(Chrysanthemum nankingense)与甘菊(Chrysanthemum lavandulifolium)种间杂种的真实性和遗传多样性。结果表明,131个杂交F1代单株中,122个为真杂种,真杂种率为93.13%。42对SSR引物组合在菊花脑、甘菊及其122个F1杂种中扩增得到123个多态性条带,平均每对引物扩增出3条多态性条带;18对SRAP引物组合扩增得到55个多态性条带,平均每对引物扩增出3条多态性条带。菊花脑 × 甘菊种间F1杂种之间的遗传相似系数介于0.44 ~ 0.90,表型变异系数在10.16% ~ 16.67%之间,且存在超亲个体,说明种间杂种的遗传变异丰富;杂种的叶片表型偏向于母本。基于表型性状和SSR、SRAP分子标记的UPGMA聚类分析将亲本和122个杂交后代都分为7类,绝大多数杂种后代与母本菊花脑聚在一起,而父本甘菊和少部分杂种株系分别单独聚在一起,表明F1代与母本更为相似。母本与种间杂种的平均遗传相似性系数(0.62)高于父本(0.54),说明该杂种群体在遗传上更接近母本,与表型观察结果相吻合。

关键词: 菊花近缘种, 菊花脑, 甘菊, 种间杂交, 杂种鉴定, 分子标记, 遗传多样性

Abstract:

SSR and SRAP markers and morphological traits were used to analyse the genetic diversity of the interspecific F1 population derived from the cross between diploid Chrysanthemum related species C. nankingense and C. lavandulifolium. The results showed that 122 of 131 F1 hybrids were true hybrids,and the true hybrids rate was 93.13%. Forty-two SSR primer combinations amplified 123 polymorphic bands in C. nankingense,C. lavandulifolium and 122 F1 hybrids,with an average of 3 polymorphic bands per primer combination. Eighteen SRAP primer combinations amplified 55 polymorphic bands,with an average of 3 polymorphic bands per primer combination. The genetic similarity coefficients between the interspecific hybrids of C. nankingense × C. lavandulifolium were between 0.44 and 0.90,and the phenotypic variation coefficients of interspecific hybrids were between 10.16% and 16.67%. Some transgressive individuals were observed,indicating an abundant genetic variation in the interspecific F1 population. In addition,it was observed that the interspecific hybrids segregated towards the female parent in leaf morphology. UPGMA cluster analysis based on phenotypic traits and molecular markers both classified parents and 122 hybrid progenies into 7 groups. Most of the hybrid progenies were clustered together with the female parent(C. nankingense),while the male parent(C. lavandulifolium)and a few hybrid lines were clustered separately. The clustering results showed that the F1 hybrids were more similar to the female parent. The average coefficient of genetic similarity(0.62)between female and interspecific hybrids was higher than that of male parent(0.54). This indicates that the interspecific hybrids are closer to female parent in heredity,consistent with the results of morphological analysis.

Key words: Chrysanthemum related species, C. nankingense, C. lavandulifolium, interspecific hybridization, hybrid identification, molecular marker, genetic diversity

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