关键词: 羽衣甘蓝, 白菜型油菜, 硫代葡萄糖苷, 超高效液相色谱—飞行时间质谱, 黄酮醇, 生物合成, 代谢调控, 分子机制, 基因工程

Abstract:

A method for analysis of glucosinolates in cruciferous vegetable was developed by using UHPLC–Triple–TOF–MS. Vegetable samples were extracted using 70% methanol and injected after concentration. UPLC BEH C₁₈ column was selected with acetonitrile–water（both 0.1% formic acid）as mobile phase. Chromatographic separation was achieved under gradient elution in 10 min. In ESI negative ion mode，TOF–MS scan–IDA–Product ion scan was performed to acquire both MS and MS/MS information from one injection. Based on high resolution TOF–MS，accurate masses of molecular ions and fragment ions were obtained for high accuracy-identification. This method was applied to the seeds of Yellow Sarson（Brassica rapa ssp. trillocularis）and the leaves of kale（Brassica oleracea）. Finally，24 and 15 glucosinolates were analyzed in the seeds and leaves，respectively. The results demonstrated that this method has distinct advantages of high sensitivity，good accuracy and high throughput，which is suitable for analysis of glucosinolates in cruciferous vegetables.

Key words: kale, Yellow Sarson, glucosinolate, UHPLC–Triple–TOF–MS, Flavonol, biosynthesis, regulation, molecular mechanism, genetic engineering