Genetic Relationships of Diospyros kaki and Related Diospyros Species Using Chloroplast DNA PCR-RFLP Markers

ACTA HORTICULTURAE SINICA ›› 2014, Vol. 41 ›› Issue (12): 2474-2480.

• Research Notes • Previous Articles Next Articles

Genetic Relationships of Diospyros kaki and Related Diospyros Species Using Chloroplast DNA PCR-RFLP Markers

LIANG Jin-jun，LIANG Yu-qin，and FU Jian-min

China Paulownia Research Center，Non-timber Forest Research and Development Center，Chinese Academy of Forestry，Zhengzhou 450003，China

Online:2014-12-25 Published:2014-12-25

Abstract

Abstract: Five special primer pairs of chloroplast genome were used to amplified chloroplast DNA （cpDNA）regions in 8 Diospyros species including 32 genotypes. The products were digested by seven restriction enzymes. The results showed high interspecific cpDNA variations within the genus Diospyros. Twenty-two D. kaki genotypes were detected no visually variation in this study. D. kaki had closely relationship with‘Jinzaoshi’，followed by D. oleifera，D. artrotricha，D. lotus and D. glaucifolia，but distant from D. virginiana and D. cathayensis in diagram based on the UPGMA cluster analysis by NTSYSpc 2.10e program. Wild persimmon（Zhejiang）is a variety varietas of persimmon. The result showed that wild persimmon（Zhejiang）is not closely similar to D. kaki.

Key words: Diospyros kaki, cpDNA, PCR-RFLP, genetic diversity, genetic relationship

CLC Number:

S 665.2

LIANG Jin-Jun, LIANG Yu-Qin, FU Jian-Min. Genetic Relationships of Diospyros kaki and Related Diospyros Species Using Chloroplast DNA PCR-RFLP Markers[J]. ACTA HORTICULTURAE SINICA, 2014, 41(12): 2474-2480.

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Genetic Relationships of Diospyros kaki and Related Diospyros Species Using Chloroplast DNA PCR-RFLP Markers

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