Abstract: Through the identification and bioinformatics analysis of Osmanthus fragrans，and the expression analysis of related genes during flower opening，the key genes that respond to the relatively low temperature regulation of osmanthus flower opening and coloring were obtained. Using transcriptome data to analyze osmanthus R2R3-MYB genes，a total of 35 R2R3-MYBs were obtained. These genes all contained R2R3 domains and were highly conserved. The MYB transcription factors were treated in 19 ℃（flowers can open）and 23 ℃（flowers can not open）and the relative expression of different tissues was found that OfMYB1，OfMYB12，OfMYB14，OfMYB23 and OfMYB24 with the opening of O. fragrans，the expression increased gradually. The expression of OfMYB1 and OfMYB14 was the highest among the petals in flowering stage，indicating that they might be involved in the process of osmanthus coloring. OfMYB4，OfMYB5，OfMYB17，OfMYB28，OfMYB34 expression increased first and then decreased during flower opening，which indicated that OfMYB4，OfMYB5，OfMYB17，OfMYB28 and OfMYB34 could be involved in the regulation of the process of flower opening.

Key words: Osmanthus fragrans, MYB transcription factor, ambient temperature, flower opening, gene expression