Establishment of Resistance Evaluation System of Banana to Fusarium oxysporum f. sp. cubense Tropical Race 4

doi:10.16420/j.issn.0513-353x.2015-0781

ACTA HORTICULTURAE SINICA ›› 2016, Vol. 43 ›› Issue (5): 876-884.doi: 10.16420/j.issn.0513-353x.2015-0781

Establishment of Resistance Evaluation System of Banana to Fusarium oxysporum f. sp. cubense Tropical Race 4

ZUO Cun-wu1，LI Bin2，LI Chun-yu1，WEI Yue-rong1，HU Chun-hua1，DENG Gui-ming1，KUANG Rui-bin1，Yang Qiao-song1，and YI Gan-jun1,*

（1Institute of Fruit Tree Research，Guangdong Academy of Agricultural Sciences，Key Laboratory of South Subtropical Fruit Biology and Genetic Resource Utilization，Ministry of Agriculture，Guangzhou 510640，China；2Collage of Life Science，South China Agricultural University，Guangzhou 510640，China）

Online:2016-05-25 Published:2016-05-25

Abstract

Abstract:

Fusarium wilt is the most destructive disease of banana production，which caused by Fusarium oxysporum f. sp. cubense（Foc）. Foc tropical race 4（Foc TR4）is the most pathogenic type. Rapid and accurate evaluation system for resistance of banana to Foc TR4 is essential for both basic research and banana breeding. In this study，we compared the incubation effects of immersing root method and improved drenching root method，and the detection effectives of two primers. The results showed that the resistance of banana could be evaluated accurately by using improved immersing root method with concentration of 1 × 10⁴. For molecular detection，corms with index of disease up to scale 3 could be amplified by first PCR using the special primers Foc TR4 F/R，and all diseased bubs of ID scale up to 1 could be de detected by Sub-PCR.

Key words: banana, Fusarium wilt, Fusarium oxysporum f. sp. cubense tropical race 4（Foc TR4）, resistance evaluation, molecular detection

CLC Number:

S 668.1

ZUO Cun-wu1，LI Bin2，LI Chun-yu1，WEI Yue-rong1，HU Chun-hua1，DENG Gui-ming1，KUANG Rui-bin1，Yang Qiao-song1，and YI Gan-jun1,*. Establishment of Resistance Evaluation System of Banana to Fusarium oxysporum f. sp. cubense Tropical Race 4[J]. ACTA HORTICULTURAE SINICA, 2016, 43(5): 876-884.

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Establishment of Resistance Evaluation System of Banana to Fusarium oxysporum f. sp. cubense Tropical Race 4

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