关键词: 华重楼, 种子, GA3, IAA, 转录组测序, 激素信号转导通路, 激素合成途径

Abstract: The experiment used transcriptome sequencing to analyze differentially expressed genes（DEGs）in hormone synthesis and hormone signal transduction pathways. The mechanism of the combination of GA3 and IAA on the germination of seeds of Paris polyphylla var. chinensis was preliminarily revealed. The results found that the germination rate of seeds of P. polyphylla var. chinensisreached 87.33% after 8 months of treatment with 600 mg · L-1 GA3 + 40 mg · L-1 IAA. Comparing the DEGs from transcriptome sequencing with the KEGG database. We found that treating P. polyphylla var. chinensis seeds with exogenous GA3 and IAA of could down-regulated the GA2ox and up-regulate the GA20ox. In the GA signal transduction pathway，the GID1 was down-regulated and the PIF4 was up-regulated. It was speculated that the GA signal transduction was enhanced by the combined increase action of the exogenous GA3 and the endogenous GA. In the IAA synthesis pathway，the exogenous IAA up-regulated the YUCCA and ALDH. In the IAA signal transduction pathway，the auxin influx carrier gene AUX1，AUX/IAA，and SAUR et al were up-regulated. It was speculated that the IAA signal transduction was enhanced by the combined increase of the exogenous IAA and the endogenous IAA. Thus，the seed germination promoted by the combination of GA3 and IAA was associated with the GA and IAA synthesis and the signal transduction.

Key words: Paris polyphylla var. chinensis, seed, GA3, IAA, transcriptome sequencing, hormone signal transduction pathway, hormone synthesis pathway