园艺学报 ›› 2020, Vol. 47 ›› Issue (11): 2145-2160.doi: 10.16420/j.issn.0513-353x.2020-0064

• 研究论文 • 上一篇    下一篇


赵慧霞1,李凤霞2,郭 瑞1,陈禅友1,*   

  1. 1江汉大学生命科学院,武汉 430056;2中国农业科学院烟草研究所,山东青岛 266000
  • 出版日期:2020-11-25 发布日期:2020-12-07
  • 基金资助:

Genome-wide Characterization and Expression Analysis of GRF Gene Family in Capsicum

ZHAO Huixia1,LI Fengxia2,GUO Rui1,and CHEN Chanyou1,*   

  1. 1School of Life Science,Jianghan University,Wuhan 430056,China;2Tobacco Research Institute,Chinese Academy of Agricultural Sciences,Qingdao,Shandong 266000,China
  • Online:2020-11-25 Published:2020-12-07

摘要: 生长调节因子(Growth-regulating factor,GRF)是一类植物特异的转录因子,其作用涉及植物的生长发育、胁迫和激素信号响应等各个方面。目前还没有关于辣椒GRF的相关报道。利用BLASTP和隐马科夫模型(HMM)在辣椒属(Capsicum)5个参考基因组数据库鉴定了52个GRF家族成员。通过分析发现辣椒GRF转录因子长度为200 ~ 745 aa,分子量23 ~ 81 kD,理论等电点5.9 ~ 9.5。保守结构域和基序分析发现,大多数辣椒GRF N端具有QLQ和WRC保守结构域,C端至少具有FFD、TQL和GGPL结构域之一;少数GRF N端仅具有WRC结构域,C端缺乏保守序列。系统进化分析发现,辣椒GRF转录因子分为5个群,位于同一群的GRF保守结构域位置、基序组成、基因结构相似,辣椒GRF与番茄GRF具有一一对应的进化关系。转录组数据分析表明,CaGRF8在辣椒果实发育早中期表达量高,CaGRF4在果实发育后期表达量较高;低温、干旱、盐和赤霉素处理后辣椒幼苗CaGRF表达量发生明显变化,CaGRF8受到赤霉素诱导表达量升高,而CaGRF4的表达被赤霉素抑制。

关键词: 辣椒, GRF基因家族, 表达分析, GA3

Abstract: The Growth-Regulating Factors(GRFs)are plant-specific transcription factors that play pivotal roles in growth and development,stress and phytohormone response. However,the GRF family in Capsicum remains to be investigated. In this study,genome-wide retrievation of GRFs in five Capsicum reference genomes was carried out using BLASTP and HMM(Hidden Markov Model),and a total of 52 GRFs were identified. Further analysis was performed regarding their characterization,phylogenetic relationship,tissues expression profiles and expression level upon GA3 treatment. The length of pepper GRF proteins ranges from 200 to 745 amino acids(aa),with the molecular weight(MW)of 23–81 kD,and the theoretical isoelectric point of 5.9–9.5. The majority of GRF proteins harbor the conservative QLQ and WRC domains at N-terminal end,and one or more FFD,TQL,or GGPL motifs in the C-terminus. A few GRFs contain only the WRC domain in the N-terminus and the non-conserved

Key words: Capsicum, GRF gene family, expression analysis, GA3