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园艺学报 ›› 2019, Vol. 46 ›› Issue (10): 1973-1988.doi: 10.16420/j.issn.0513-353x.2019-0028

• 研究论文 • 上一篇    下一篇

甘蓝PMEI家族基因结构、基因组分布和表达分析

白晓璟1,王玉奎1,左同鸿1,刘倩莹1,廉小平2,张贺翠1,张以忠1,蒲 敏1,罗绍兰1,朱利泉1,*   

  1. 1西南大学农学与生物科技学院,重庆 400700;2西南大学园艺园林学院,重庆 400700
  • 出版日期:2019-10-25 发布日期:2019-10-25
  • 基金资助:
    国家自然科学基金项目(31572127);西南大学基本科研业务费专项资金项目(XDJK2017D073)

The Strcture,Genome-Wide Distribution and Expression Analysis of Pectin Methylesterase Inhibitor Genes in Brassica oleracea

BAI Xiaojing1,WANG Yukui1,ZUO Tonghong1,LIU Qianying1,LIAN Xiaoping2,ZHANG Hecui1,ZHANG Yizhong1,PU Min1,LUO Shaolan1,and ZHU Liquan1,*   

  1. 1College of Agronomy and Biotechnology,Southwest University,Chongqing 400700,China;2College of Horticulture and Landscape Architecture,Southwest University,Chongqing 400700,China
  • Online:2019-10-25 Published:2019-10-25

摘要: 为了弄清甘蓝PMEI家族基因成员数、基因结构和分布及其与自交不亲和性的关系,利用转录组测序和相关生物信息学方法对其进行了基因结构分析和全基因组鉴定,对家族基因进行了表达谱分析,并通过实时荧光定量PCR技术验证了基因的表达情况。结果表明,甘蓝中PMEI家族包含101个基因,分为11组,其成员不均匀分布在9条染色体上,主要通过全基因组三倍体复制(whole genome triplication,WGT)和串联复制事件进行倍增,与拟南芥在1 556万年前发生分化。数字表达谱分析发现在101个基因中,BoPMEI56、BoPMEI54、BoPMEI32、BoPMEI29、BoPMEI78、BoPMEI30、BoPMEI57、BoPMEI58和BoPMEI84这9个基因在自花和异花授粉后表达趋势有明显差异。荧光定量 PCR 结果显示,其在自花授粉和异花授粉的表达变化趋势与转录组分析结果基本一致。这些结果表明:BoPMEI家族的101个成员中,可能至少有9个基因以不同方式参与调控甘蓝自花和异花授粉后的反应过程,可能与甘蓝自交不亲和性有关。

关键词: 甘蓝, BoPMEI基因家族, 生物信息学, 表达分析

Abstract: In order to clarify the number of members,structures and distribution of BoPMEI family,and find out the relationship between BoPMEI family and self-incompatibility,transcriptome data and bioinformatics approaches were used to analyze the gene structure and identify the whole genome. The expression profile of BoPMEI family genes was analyzed,and it was verified by quantitative real-time PCR. The results showed that a total of 101 BoPMEI genes were identified and they were divided into 11 groups. The expansion of BoPMEI family genes distributed unevenly on 9 chromosomes were mainly caused by whole genome triplication and tandem duplication. The divergence time was determined to be 15.56 million years ago. Among the 101 genes,9 genes,such as BoPMEI56,BoPMEI54,BoPMEI32,BoPMEI29,BoPMEI78,BoPMEI30,BoPMEI57,BoPMEI58 and BoPMEI84,had significant expression differences. qRT-PCR analysis revealed that the mRNA expression patterns of BoPMEI was similar to the RNA-seq analysis. These results indicated that at least 9 of 101 gene members were involved in the regulation of self-pollination and cross-pollination of Brassica oleracea in different ways and may be related to the self-incompatibility of Brassica oleracea.

Key words: Brassica oleracea, BoPMEI family genes, bioinformatics, expression analysis

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