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园艺学报 ›› 2017, Vol. 44 ›› Issue (1): 161-169.doi: 10.16420/j.issn.0513-353x.2016-0453

• 研究报告 • 上一篇    下一篇

广西甜茶种质资源遗传多样性的ISSR分析

陈宗游1,2,3黄夕洋2  辉1,2,*王满莲2柴胜丰1,2   

  1. 1广西植物功能物质研究与利用重点实验室,广西桂林 541006;2广西壮族自治区中国科学院广西植物研究所,广西桂林 541006;3广西大学农学院,南宁 530005

ISSR Analysis on Genetic Diversity for Germplasm Resources of Rubus suavissimus

CHEN Zongyou1,2,3,HUANG Xiyang2,TANG Hui1,2,*,WANG Manlian2,and CHAI Shengfeng1,2   

  1. 1Guangxi Key Laboratory of Functional Phytochemicals Research and UtilizationGuilinGuangxi 541006,China2Guangxi Institute of BotanyGuangxi Zhuang Autonomous Region and the Chinese Academy of SciencesGuilinGuangxi 541006,China3College of AgronomyGuangxi UniversityNanning 530005,China
  • Online:2017-01-25 Published:2017-01-25

摘要:

采用ISSR分子标记对广西甜茶(Rubus suavissimus S. Lee)种质资源的遗传多样性进行分析。14条引物在4个广西甜茶种群总共85个个体上共检测到164个位点,其中多态性位点148个,多态位点百分率(PPL)为90.24%,物种水平Nei氏基因多样性指数(He)和香农多样性指数(Ho)分别为0.2514和0.3875,表现出较高的遗传多样性;而在种群水平上,PPL57.32% ~ 68.90%(均值为61.74%)He0.1831 ~ 0.2161(均值为0.1958)Ho0.2776 ~ 0.3264(均值为0.2956)遗传多样性为中等偏低水平Nei氏遗传多样性分析和AMOVA分析表明,广西甜茶的遗传变异主要存在于种群内,但其种群间存在极显著的遗传变异(P < 0.01),其种群遗传分化达到极大水平(Φst = 0.262 > 0.25)。Mantel检测表明,种群间的遗传距离和地理距离之间存在显著相关性(r = 0.967,P < 0.05)。UPGMA聚类分析和主成分分析(PCA)结果一致,4个广西甜茶种群可分成2个遗传分支。鉴于广西甜茶在种群水平上表现出中等偏低的遗传多样性水平,且其生境受到破坏以及野生资源受到滥挖乱采,广西甜茶面临种群退化的威胁,对其应加强保护,做到保护和利用并举。

关键词: 广西甜茶, 遗传多样性, ISSR, 遗传分化, 基因流

Abstract:

In this study,the genetic diversity of germplasm resources of Rubus suavissimus Lee was analyzed by ISSR(Inter simple sequence repeats)molecular marker technique. Fourteen ISSR primers were used to amplify 85 DNA samples which were extracted from the individuals of four natural populations of R. suavissimus. A total of 164 bands were detected,of which 148 bands were polymorphic with a polymorphic proportion of 90.24%. At the species level,the Nei’s genetic diversity(He)and and Shannon’s information index(Howere 0.2514 and 0.3875,respectively. R. suavissimus maintained a relatively high genetic variability at the species level.PPL range from 57.32% to 68.90% with a mean of 61.74%He range from 0.1831 to 0.2161 with a mean of 0.1958,Horange from0.2776 to 0.3264 with a mean of 0.2956. And there was a lower middle genetic variability at the population level.Although most variation consistently originated from the interior of populations which was detected based on Nei’s genetic diversity analysis and analysis of molecular variance,a significantly genetic differentiation existed among populations(P < 0.01,Φst = 0.262 > 0.25). A mantel test indicated there was a significant relationship between genetic distance and geographic distance among the populations studied(r = 0.967,P < 0.05). The result of UPGMA clustering analysis was basically similar to that of the principle coordinate analysis(PCA),and four populations could be classified into two distinct genetic groups. Given the lower middle genetic variability at the population level and a serious habitat destruction and indiscriminate digging Luancai in the species,the populations of R. suavissimus faces the threat of degradation,and it should be taken practical measures to enhance the protection and rational utilization of resources for the species. At the population levelS.

Key words: Rubus suavissimus, genetic diversity, ISSR, genetic differentiation, gene flow

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