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园艺学报 ›› 2017, Vol. 44 ›› Issue (8): 1505-1516.doi: 10.16420/j.issn.0513-353x.2017-0155

• 研究论文 • 上一篇    下一篇

壳聚糖对番茄穴盘苗根际细菌群落结构的改良作用

董春娟,李 亮,王玲玲,尚庆茂*   

  1. (中国农业科学院蔬菜花卉研究所,农业部园艺作物生物学与种质创制重点实验室,环渤海湾地区设施蔬菜优质高效生产协同创新中心,北京 100081)
  • 出版日期:2017-08-25 发布日期:2017-08-25

Improvement Effects of Chitiosan on Rhizosphere Bacterial Community Structure of Tomato Plug Seedlings

DONG Chunjuan,LI Liang,WANG Lingling,and SHANG Qingmao*   

  1. (Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences;Key Laboratory of Biological and Genetic Improvement of Horticultural Crops,Ministry of Agriculture;Collaborative Innovation Center of Protected Vegetables Surround Bohai Gulf Region,Beijing 100081,China)
  • Online:2017-08-25 Published:2017-08-25

摘要: 以草炭、蛭石、珍珠岩混合物料为基质,并添加壳聚糖,进行番茄穴盘育苗试验,测定幼苗生长发育参数,并采用454焦磷酸测序技术分析穴盘苗根际细菌的种群变化。结果表明:添加壳聚糖对基质pH值无显著影响,但显著降低了生长后期基质EC值。番茄穴盘苗根际细菌优势菌群包括丛毛单胞菌科食酸菌属(8.92%)、丰祐菌科丰佑菌属(5.03%)以及草酸杆菌科Massilia属(2.43%)等。基质添加壳聚糖显著促进番茄穴盘苗的生长。壳聚糖引起根际细菌群落的丰富度和多样性降低,优势细菌主要包括丛毛单胞菌科Pelomonas属(13.52%)、假单胞菌科纤维弧菌属(8.21%)、根瘤菌科根瘤菌属(2.91%)和亚硝化单胞菌科亚硝化螺菌属(1.04%)等,这些优势细菌多具有植物促生潜力。添加壳聚糖对根际细菌具有定向调控作用,可以显著改善番茄穴盘苗根际基质的细菌群落结构。

关键词: 番茄, 基质, 壳聚糖, 根际, 细菌多样性

Abstract: In order to investigate the effects of chitosan on rhizosphere micro-environment of tomato plug seedlings,chitosan was applied to the substrate before sowing,and the rhizosphere bacterial diversities were detected by 454 pyrosequencing. The results showed that pH value of substrate did not change upon chitosan application,a decreased EC value was detected on 40 days after sowing. In the rhizosphere of tomato plug seedlings,the major bacteria genera included Acidovorax(8.92%),Opitutus(5.03%),and Massilia(2.43%). Application of chitosan could promote the growth of tomato seedlings. After chitosan application,the rhizosphere bacterial comunities changed markedly,and the bacterial richness and diversity decreased significantly. The dominant genera included Pelomonas(13.52%),Cellvibrio(8.21%),Rhizobium(2.91%),and Nitrosospira(1.04%). Most of these bacteria were potential plant growth-promoting bacteria. Taken together,chitosan could selectively promote the proliferation of benifical bacteria,and thereby improve the bacterial community in the rhizosphere substrate of tomato plug seedlings.

Key words: tomato, substrate, chitosan, rhizosphere, bacterial diversity

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