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园艺学报 ›› 2015, Vol. 42 ›› Issue (7): 1385-1392.doi: 10.16420/j.issn.0513-353x.2014-1146

• 研究报告 • 上一篇    下一篇

荔枝类甜蛋白基因的克隆与表达分析

王树军,冯超,王凌云,李焕苓,刘保华,王家保   

  1. 1中国热带农业科学院环境与植物保护研究所,海口 571101;2海南大学农学院,海口 570228
  • 出版日期:2015-07-25 发布日期:2015-07-25
  • 基金资助:

    国家现代农业产业技术体系建设专项资金项目(CARS-33-02);国家自然科学基金项目(31272137)

Cloning and Expression Analysis of Thaumatin-like Protein Gene from Litchi chinenesis

WANG Shu-Jun, FENG Chao, WANG Ling-Yun, LI Huan-Ling, LIU Bao-Hua, WANG Jia-Bao   

  1. 1Environment and Plant Protection Institute,Chinese Academy of Tropical Agricultural Sciences,Haikou 571101,China;2 College of Agriculture,Hainan University,Haikou 570228,China
  • Online:2015-07-25 Published:2015-07-25

摘要: 以‘妃子笑’荔枝(Litchi chinenesis)为试材,通过PCR方法克隆了荔枝类甜蛋白基因LcTLP(登录号JF682821)的cDNA全长和完整开放阅读框(ORF)对应的gDNA序列。序列分析表明:该基因无内含子,cDNA序列含有一个672 bp的ORF,编码223个氨基酸残基序列。荧光定量PCR结果表明:LcTLP在花中表达量最高,其次是果皮,在果肉中表达量最低;在果实发育过程中,果皮中LcTLP表达量先上升后下降,采后果皮中的表达量高于采前,炭疽菌侵染诱导LcTLP的表达,失水和低温能抑制LcTLP的表达。

关键词: 荔枝, 类甜蛋白基因, 基因克隆, 基因表达

Abstract: The full length of cDNA sequence and the genome sequence corresponding to the open reading frame(ORF)of a thaumatin-like protein gene(LcTLP,Accession No:JF682821)were obtained from‘Feizixiao’litchi(Litchi chinensis Sonn.‘Feizixaio’)by PCR method. The cDNA sequence was contained an ORF with 672 bp in length,encoding a polypeptide with 223 amino acid residues. There was no intron in LcTLP genome sequence. Quantitative real-time PCR results showed that the expression of LcTLP was the highest in the flower,followed by in the pericarp,and the lowest in the pulp. During the fruit development,the expression of LcTLP in the pericarp increased firstly,and then decreased. The expression of the gene in the pericarp of postharvested fruit was higher than that of developed fruit. The expression of LcTLP in the pericarp could be induced by Colletotrichum infection;However,it could be inhibited by water loss and low temperature.

Key words: litchi, thaumatin-like protein, gene cloning, gene expression

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