园艺学报 ›› 2016, Vol. 43 ›› Issue (1): 25-37.doi: 10.16420/j.issn.0513-353x.2015-0324

• 果树 • 上一篇    下一篇


高 源,王 昆*,王大江,龚 欣,刘立军,刘凤之   

  1. 中国农业科学院果树研究所,农业部园艺作物种质资源利用重点实验室,辽宁兴城 125100
  • 出版日期:2016-01-25 发布日期:2016-01-25
  • 基金资助:


Molecular ID Establishment of Apple Cultivars by TP-M13-SSR

GAO Yuan,WANG Kun*,WANG Da-jiang,GONG Xin,LIU Li-jun,and LIU Feng-zhi   

  1. Research Institute of Pomology,Chinese Academy of Agricultural Sciences/Key Laboratory of Biology and Genetic Improvement of Horticultural Crop Germplasm Resources Utilization,Ministry of Agriculture,Xingcheng,Liaoning 125100,China
  • Online:2016-01-25 Published:2016-01-25



关键词: 苹果, 栽培品种, TP-M13-SSR, 指纹图谱, 分子身份证


A total of 314 apple cultivars from 19 countries all over the world in the national pear and apple repository of Xingcheng were studied with tailed primer M13 microsatellite markers(TP-M13-SSR). Analysis was made to establish the 314 apple cultivars molecular ID. The results showed that by using 16 selected SSR markers,357 alleles were detected with a mean value of 22.3 alleles. Based on number of alleles and Shannon’s information index,more and more loci were added to distinguish all the apple accessions,and finally six core primers at least were screened to establish germplasms molecular ID. Base on genetic fingerprints at six loci,loci that amplified by each marker were coded,then the code were combined as a molecular ID. Using barcode technology molecular ID can be transferred into barcode ID that can be scaned quickly by machine. Every apple germplasm obtain it’s special molecular ID. The purpose that using least primers distinguishes most apple germplasms has been come true.

Key words: apple, cultivar, TP-M13-SSR, genetic fingerprints, molecular ID