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园艺学报 ›› 2013, Vol. 40 ›› Issue (8): 1567-1573.

• 研究报告 • 上一篇    下一篇

香蕉抗逆相关基因MaERF的克隆与表达分析

张俊芳1,2,3,黄俊生3,丛汉卿2,李志英2,徐 立2,*   

  1. (1海南大学农学院,海口 570228;2中国热带农业科学院热带作物品种资源研究所,农业部华南作物基因资源与种质创制重点实验室,海南儋州 571737;3中国热带农业科学院环境与植物保护研究所,海口 571101)
  • 收稿日期:2013-03-07 出版日期:2013-08-25 发布日期:2013-08-25

Cloning and Expression Analysis of a New Stress-resistant ERF Gene from Banana

ZHANG Jun-fang1,2,3,HUANG Jun-sheng3,CONG Han-qing2,LI Zhi-ying2,and XU Li2,*   

  1. (1 College of Agriculture,Hainan University,Haikou 570228,China;2Institute of Tropical Crop Genetic Resources,Chinese Academy of Tropical Agricultural Sciences,Ministry of Agriculture Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern China,Danzhou,Hainan 571737,China;3Enviornment and Plant Protection Institution,Chinese Academy of Torpical Agricultural Sciences,Haikou 571101,China)
  • Received:2013-03-07 Online:2013-08-25 Published:2013-08-25

摘要: 根据已获得的AP2/ERF基因片段,通过RACE方法获得一个香蕉ERF基因完整编码区序列,命名为MaERF。该基因cDNA全长1 611 bp,其中开放阅读框1 128 bp,编码376个氨基酸,包含一个保守的AP2/ERF结构域,基因组序列全长2 881 bp,含有一个内含子,剪切位点符合“GT-AG”规则;系统进化树表明该基因属于植物ERF转录因子家族的B2亚群。半定量RT-PCR分析表明,该基因在香蕉各个器官中均有表达,其中在根和叶中的表达量较低,而在果实和花中的表达较高;实时荧光定量Real-time PCR检测表明,受尖孢镰刀菌侵染及低温胁迫后,香蕉中该基因表达明显上调,推测其在香蕉胁迫反应中可能发挥重要作用。

关键词: 香蕉, ERF基因, 克隆, 表达分析

Abstract: A full length cDNA named MaERF which was cloned from banana on the basis of a AP2/ERF fragment combined with RACE technology. The full cDNA of this gene was 1 611 bp,the open reading frame was 1 128 bp and coded a polypeptide of 376 amino acids. This gene had one typical AP2/ERF domain. The genome sequence full-length of this gene was 2 881 bp,and contained one intron splice site according to the rules of the“GT-AG”. The phylogenetic tree shows that the gene belongs to B2 subsets of ERF genes,and they are more involved in plant stress reactions. Semi-quantitative RT-PCR analysis showed that the gene was expressed in various organs of the banana,with lower the amount of expression in the roots and leaves,while high expression in fruit and flowers;Fluorescence quantitative real-time PCR detection showed this gene had high expression with Fusarium oxysporum and low temperature stress,so this gene may play an important role in bananas stress reaction.

Key words: banana, ERF gene;clone, expression analysis