关键词: 葡萄, 葡萄蚕豆萎蔫病毒, 实时荧光定量RT-PCR, RT-PCR

Abstract: A SYBR GreenⅠRT-qPCR method for GFabV detection was established，an excellent linear correlation（0.998）and amplification efficiency（103.8%）were obtained from standard curve. The sensitivity of the method was 1 000 times higher than conventional RT-PCR，and had a strong specificity. The established RT-qPCR and conventional RT-PCR were used to detect GFabV in 316 grapevine samples from different development stages and different positions，and the results showed that the detection rate of RT-qPCR（96.8%）was apparently higher than conventional RT-PCR（70.8%）. The detection rate of dormant branches using RT-qPCR was 100%，and for other positions detection were all above 92%，which were apparently higher than conventional RT-PCR（42%–97%）. The detection rates using RT-qPCR（85%–95%）were higher than conventional RT-PCR（70%–90%）in these samples from different development stages. Compared with conventional RT-PCR，the newly established RT-qPCR has obvious superiority in detection of various samples.

Key words: grape, Grapevine fabavirus, RT-qPCR, RT-PCR