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园艺学报 ›› 2020, Vol. 47 ›› Issue (10): 1947-1955.doi: 10.16420/j.issn.0513-353x.2020-0047

• 研究论文 • 上一篇    下一篇

长沙地区萝卜病毒病病原鉴定与萝卜种质抗病性评价

邱孝军,谭群运,肖清明,梅时勇*,张冀芳*   

  1. 中国农业科学院麻类研究所,长沙 410205
  • 出版日期:2020-10-25 发布日期:2020-11-10
  • 基金资助:
    国家重点研发计划项目(2017YFD0101806);全国农业科研杰出人才项目;中国农业科学院农科英才计划项目(CAASQNYC- KYYJ-38)

Pathogen Identification of Virus Disease and Evaluation for Germplasm Disease Resistance in Radish

QIU Xiaojun,TAN Qunyun,XIAO Qingming,MEI Shiyong*,and ZHANG Jifang*   

  1. Institute of Bast Fiber Crops,Chinese Academy of Agricultural Sciences,Changsha 410205,China
  • Online:2020-10-25 Published:2020-11-10

摘要: 采用小RNA深度测序技术对长沙地区萝卜(Raphanus sativus L.)病毒病病原进行测序鉴定,进一步设计特异性引物对其进行RT-PCR(reverse transcription PCR)检测验证。通过苗期人工接种鉴定方法对60份萝卜种质资源进行接种,并对其进行表型抗性鉴定和RT-PCR检测。结果显示,该地区萝卜被4种病毒病复合侵染,分别是芜菁花叶病毒(turnip mosaic virus,TuMV)、Brassica napus RNA virus 1(BnRV1)、芸薹属黄化病毒(Brassica yellows virus,BrYV)、Raphanus sativus cryptic virus 3(RasCV3)。首次在萝卜中检测到BnRV1。根据表型筛选出1份高抗材料和1份抗病材料。接种的60份种质RT-PCR检测结果显示,TuMV为主要病原,检出率100%,2种以上病原复合侵染率为83.33%。

关键词: 萝卜, 病毒病, 小RNA测序, 抗性评价

Abstract: In this study,the pathogen of radish virus disease in Changsha was identified by small RNA deep sequencing technology,and specific primers were further designed to detect and verify the pathogen by reverse transcription PCR. Further,60 core germplasms were identified by artificial inoculation at the seedling stage,and then all were subjected to RT-PCR detection and phenotypic resistance identification. The results showed that the radishes in this area were infected by a combination of four viral diseases,namely turnip mosaic virus(TuMV),Brassica napus RNA virus 1(BnRV1),Brassica yellows virus(BrYV),Raphanus sativus cryptic virus 3(RasCV3). BnRV1 was detected in radishes for the first time. One high resistance and one disease resistance accession was selected according to the phenotype identification. The specific bands of TuMV,BnRV1,BrYV and RasCV3 were abtained by RT-PCR with specific primers in 60 core germplasms,indicating that all the tested accessions were infected by these four pathogens. TuMV is the main pathogen,and the detection rate is 100%. The composite infection rate of two or more pathogens was 83.33%.

Key words: radish, virus disease, small RNA sequencing, resistance identification

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