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园艺学报 ›› 2020, Vol. 47 ›› Issue (5): 995-1008.doi: 10.16420/j.issn.0513-353x.2019-0552

• 综述 • 上一篇    下一篇

microRNA 参与柑橘生长发育和抗逆的研究进展

姚利晓,何永睿,陈善春*   

  1. 西南大学/中国农业科学研究院柑桔研究所,国家柑桔工程技术研究中心,国家柑桔品种改良中心,重庆 400712
  • 出版日期:2020-05-25 发布日期:2020-05-25
  • 基金资助:
    国家现代农业产业技术体系建设专项资金项目(CARS-26);重庆市基础与前沿研究项目(cstc2018jcyjAX0247);中央高校基本业务费专项(XDJK2019B018)

Research Advances of Citrus microRNAs in Plant Development and Stress Resistance

YAO Lixiao,HE Yongrui,and CHEN Shanchun*   

  1. Citrus Research Institute,Southwest University/Chinese Academy of Agricultural Sciences;National Citrus Engineering research Center;National Center for Citrus Variety Improvement,Chongqing 400712,China
  • Online:2020-05-25 Published:2020-05-25

摘要: microRNA(miRNA)是在转录后水平调控基因表达的一类非编码的小分子RNA。植物miRNA最早于2002 年在拟南芥中发现,柑橘miRNA 的研究始于2005 年,现已发现了许多保守的miRNA 和新的miRNA。对参与柑橘生长发育及对生物学胁迫和非生物学胁迫应答过程的miRNA 进行了归纳,并对cis-miR156、cis-miR396 和cis-miR3954 在柑橘中的功能研究进行了总结。与模式植物和大田作物相比,柑橘miRNA 的功能研究相对滞后,新发现miRNA 的功能有待深入研究。最后对利用柑橘特异的病毒载体和基因定点编辑技术加快柑橘miRNA 功能研究进行了展望。

关键词: 柑橘, miRNA, 生长发育, 生物和非生物胁迫, 柑橘病毒载体, CRISPR/Cas9

Abstract: microRNAs(miRNAs)are a class of endogenous small non-coding RNAs,which regulate complementary mRNAs by mediating mRNA degradation and translational repression. The research on citrus miRNAs has been conducted from 2005,three years after the first plant miRNA was identified in Arabidopsis. Many plant-conserved miRNAs and novel miRNAs have been identified in citrus,with the advancement of high-throughput sequencing technology and the improvement of bioinformatics analysis methods. These miRNAs were not only involved in the biological processes,such as citrus reproduction,flower and fruit development,but also in the resistance against biotic and abiotic stresses,through regulation of their target genes. Meanwhile,the functions of cis-miR156,cis-miR396,and cis-miR3954were summarized herein. In the present manuscript,we also prospected the usage of citrus-specific viral vectors and CRISPR/Cas9 gene editing technology to accelerate the study on function of citrus miRNAs,especially the novel miRNAs.

Key words: citrus, miRNA, growth and development, biotic and abiotic stress, citrus virus vector; CRISPR/Cas9

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