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园艺学报 ›› 2019, Vol. 46 ›› Issue (7): 1290-1302.doi: 10.16420/j.issn.0513-353x.2019-0308

• 研究论文 • 上一篇    下一篇

葡萄汁有孢汉逊酵母对采后草莓灰霉病抗性诱导机理研究

窦国霞1,蒋春号2,郭虹娜1,刘 佳1,贺沁玉1,肖红梅1,*   

  1. 1南京农业大学食品科技学院,南京 210095;2南京农业大学植物保护学院,南京 210095
  • 出版日期:2019-07-25 发布日期:2019-07-25
  • 基金资助:
    国家自然科学基金项目(31701829);江苏高校优势学科建设工程资助项目

Studies on the Resistance Induction of Hanseniaspora uvarum to Postharvest Gray Mold in Strawberry

DOU Guoxia1,JIANG Chunhao2,GUO Hongna1,LIU Jia1,HE Qinyu1,and XIAO Hongmei1,*   

  1. 1College of Food Science and Technology,Nanjing Agricultural University,Nanjing 210095,China;2College of Plant Protection,Nanjing Agricultural University,Nanjing 210095,China
  • Online:2019-07-25 Published:2019-07-25

摘要: 以采后‘红颜’草莓果实为试验材料,通过接种葡萄汁有孢汉逊酵母(Hanseniaspora uvarum)、灰葡萄孢(Botrytis cinerea)、先接种H. uvarum后挑战接种B. cinerea来探究H. uvarum对草莓采后灰霉病的生防效果及对草莓抗性诱导的相关机理。结果显示,H. uvarum可短时间内在果实伤口部位定殖并大量繁殖,对草莓灰霉病有较好的防治效果,果实病斑直径显著减小。基因表达结果显示,先接种H. uvarum后挑战接种B. cinerea处理草莓果实的水杨酸(Salicylic acid)信号通路相关基因PR1、PR5、GLU、WRKY1、NPR1和PAL表达丰度在接种后12 ~ 24 h内显著上调,并高于H. uvarum和B. cinerea单独处理,但对茉莉酸(Jasmonic acid)信号通路标志基因PDF1和JAR1无影响;先接种H. uvarum后挑战接种B. cinerea处理可以短时间内提升草莓果实抗性相关酶SOD、APX、CAT和PAL的活性;接种H. uvarum可以延缓果实冷藏期间的劣变速度,维持较高的品质。

关键词: 草莓, 灰霉病, 采后, 葡萄汁有孢汉逊酵母, 诱导抗病性, 水杨酸信号通路

Abstract: In this experiment,‘Hongyan’strawberry fruits were used as the experimental materials to explore the biocontrol efficacy and mechanism of inducing strawberry resistance by inoculation with Hanseniaspora uvarum,Botrytis cinerea,H. uvarum challenged with B. cinerea. The results showed that H. uvarum can compete for nutrition and space in the wound by large reproduction,having good biocontrol effect on the gary mold of strawberry,and the lesion diameter of strawberry decreased significantly in H. uvarum treatment. Results of gene expression abundance indicated that the WRKY1,NPR1,PAL,PR1,PR5,GLU were up-regulated within 12 h to 24 h of H. uvarum + B. cinerea treatment,which are the marker genes of SA signaling pathway,and the up-regulation was higher than that of H. uvarum or B. cinerea treatment alone. But the PDF1 and JAR1 genes of JA signaling pathway were not accumulated in each treatment. H. uvarum challenged with B. cinerea treatment can increase the SOD,APX,PAL and CAT enzyme activity in a short time,which are involved in plant defense. The H. uvarum treatment also can delay the deterioration of fruit and maintain high quality during the cold storage period.

Key words: strawberry, gray mold, postharvest, Hanseniaspora uvarum, induced resistance, salicylic acid signaling pathway

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