陕西省草莓病毒种类的LncRNA测序初步鉴定

doi:10.16420/j.issn.0513-353x.2020-0675

摘要/Abstract

摘要：

为明确陕西省草莓主要病毒种类及各种病毒在主产区的地理分布,通过LncRNA测序结合反转录聚合酶链式反应（reverse transcription PCR,RT-PCR）对陕西省草莓主产区西安、咸阳和宝鸡的7个区或县的草莓疑似感染病毒病样品进行了检测。结果表明草莓斑驳病毒（strawberry mottle virus,SMoV）和草莓镶脉病毒（strawberry vein band virus,SVBV）在7个区或县均有发生,而草莓轻型黄边病毒（strawberry mild yellow edge virus,SMYEV）、菠菜潜隐病毒（spinach latent virus,SpLV）和番茄线虫传多面体病毒（Lycopersicon esculentum nepovirus,LENV）局部发生。首次报道在草莓上检测到SpLV和LENV。

关键词: 草莓, 草莓斑驳病毒, 草莓镶脉病毒, 草莓轻型黄边病毒, 菠菜潜隐病毒, 番茄线虫传多面体病毒, LncRNA

Abstract:

In order to clarify major virus species on strawberry and their geographical distribution in main strawberry areas in Shaanxi,this study collected and detected strawberry samples from seven districts or counties in Xi’an,Xianyang and Baoji through LncRNA sequencing and reverse transcription PCR （RT-PCR）. Results showed that strawberry mottle virus（SmoV）and strawberry vein band virus（SVBV）were identified in all of the seven districts or counties,but strawberry mild yellow edge virus（SMYEV）,spinach latent virus（SpLV）and Lycopersicon esculentum nepovirus（LENV）were only identified in parts of the seven districts or counties. SpLV and LENV were firstly identified in strawberry.

Key words: strawberry, strawberry mottle virus, strawberry vein band virus, strawberry mild yellow edge virus, spinach latent virus, Lycopersicon esculentum nepovirus, LncRNA

中图分类号:

S668.4

黄倩茹, 牛永浩, 吴宽, 刘占杰, 曹孟籍, 赵磊, 吴云锋. 陕西省草莓病毒种类的LncRNA测序初步鉴定[J]. 园艺学报, 2021, 48(8): 1589-1594.

HUANG Qianru, NIU Yonghao, WU Kuan, LIU Zhanjie, CAO Mengji, ZHAO Lei, WU Yunfeng. LncRNA Sequencing Identification of Viruses Infecting Strawberry in Shaanxi[J]. Acta Horticulturae Sinica, 2021, 48(8): 1589-1594.

图/表 4

参考文献 19

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引物名称 Name of primer	引物序列（5′-3′） Primer sequence	产物大小/bp Product size	参考文献 Reference
SVBVmt	F：AAGTTTGGATCTGGATTGCG;R：CTTTCCTTCGGATGGCTGA	816	韩晓玉等,2020
SMYEVmt	F：CACCCCGAATCCGAATGTGA;R：GAGCACGCCATCGAAGAAAT	444	韩晓玉等,2020
SMoVmt	F：GCGTGAACAGTCATGTGGTT;R：TCGTGGTCGCTTACTGGATG	620	韩晓玉等,2020
SpLV-RNA2	F：GAACCACCGAAACCGAAA;R：CCACCTCAACACCAGTCATAG	436	冯黎霞等,2020
LENV	F：TCCTTATGAGGCTTTAGGTGGCA;R：AACTTCTGAGGTGAGGTGGTCC	647	本研究This study

引物名称 Name of primer	引物序列（5′-3′） Primer sequence	产物大小/bp Product size	参考文献 Reference
SVBVmt	F：AAGTTTGGATCTGGATTGCG;R：CTTTCCTTCGGATGGCTGA	816	韩晓玉等,2020
SMYEVmt	F：CACCCCGAATCCGAATGTGA;R：GAGCACGCCATCGAAGAAAT	444	韩晓玉等,2020
SMoVmt	F：GCGTGAACAGTCATGTGGTT;R：TCGTGGTCGCTTACTGGATG	620	韩晓玉等,2020
SpLV-RNA2	F：GAACCACCGAAACCGAAA;R：CCACCTCAACACCAGTCATAG	436	冯黎霞等,2020
LENV	F：TCCTTATGAGGCTTTAGGTGGCA;R：AACTTCTGAGGTGAGGTGGTCC	647	本研究This study

采样地点 Sampling region	大于200 nt的病毒片段数量 Total virus contigs > 200 nt	与已知病毒核苷酸序列同源性/% Nucleotide（nt）identity with known virus	鉴定到的病毒种类 Viruses identified
灞桥区 Baqiao District	1	98.68	SVBV
临潼县Lintong County	4	86.02 ~ 97.63	SMoV
户县Huxian County	4	98.68 ~ 99.80	SVBV
高陵县Gaoling County	4	92.48 ~ 99.39	SVBV,SMoV
咸阳市郊区 Xianyang outskirts	10	95.73 ~ 99.05	SVBV,SMoV,SpLV,LENV
凤翔县 Fengxiang County	13	91.00 ~ 99.05	SVBV,SMoV,SpLV,LENV
眉县 Meixian County	6	86.23 ~ 99.75	SVBV,SMYEV

采样地点 Sampling region	大于200 nt的病毒片段数量 Total virus contigs > 200 nt	与已知病毒核苷酸序列同源性/% Nucleotide（nt）identity with known virus	鉴定到的病毒种类 Viruses identified
灞桥区 Baqiao District	1	98.68	SVBV
临潼县Lintong County	4	86.02 ~ 97.63	SMoV
户县Huxian County	4	98.68 ~ 99.80	SVBV
高陵县Gaoling County	4	92.48 ~ 99.39	SVBV,SMoV
咸阳市郊区 Xianyang outskirts	10	95.73 ~ 99.05	SVBV,SMoV,SpLV,LENV
凤翔县 Fengxiang County	13	91.00 ~ 99.05	SVBV,SMoV,SpLV,LENV
眉县 Meixian County	6	86.23 ~ 99.75	SVBV,SMYEV

采样地点 Sampling region	检测结果（阳性数/总数） Detection results（No. of positive samples/No. of total samples）
采样地点 Sampling region	SVBV	SMYEV	SMoV	SpLV	LENV
灞桥区 Baqiao District	6/20	1/20	2/20	0/20	0/20
临潼县Lintong County	3/20	2/20	5/20	0/20	0/20
户县Huxian County	4/20	0/20	1/20	0/20	0/20
高陵县Gaoling County	5/20	0/20	3/20	0/20	0/20
咸阳市郊区Xianyang outskirts	4/20	0/20	3/20	3/20	2/20
凤翔县 Fengxiang County	5/20	0/20	3/20	3/20	3/20
眉县 Meixian County	6/30	6/30	2/30	0/30	0/30
合计 Total	33/150	9/150	19/150	6/150	5/150