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园艺学报 ›› 2020, Vol. 47 ›› Issue (3): 477-491.doi: 10.16420/j.issn.0513-353x.2019-0228

• 研究论文 • 上一篇    下一篇

欧洲报春细胞液 pH 对花色形成的作用机理初探

翟宇慧,吕嘉琪,李 想,罗小宁,李 龙,史倩倩*   

  1. 西北农林科技大学风景园林艺术学院,陕西杨凌 712100
  • 出版日期:2020-03-25 发布日期:2020-03-25
  • 基金资助:
    国家自然科学基金项目(31800599);陕西省引进博士资助经费项目(A279021832);2017 年部属高校国家级大学生创新创业训练项目(201710712029)

Effects of Cell Sap pH on the Flower Color Formation in Primula vulgaris

ZHAI Yuhui,Lü Jiaqi,LI Xiang,LUO Xiaoning,LI Long,and SHI Qianqian*   

  1. College of Landscape Architecture and Arts,Northwest A & F University,Yangling,Shaanxi 712100,China
  • Online:2020-03-25 Published:2020-03-25

摘要: 为研究欧洲报春花瓣细胞液 pH 对花色形成的作用机理,以粉、红、白、黄、蓝 5 个花色欧洲报春的花瓣为材料,采用便携式色差仪、平面型 pH 计以及 UPLC 技术对其花色值(CIE L*a*b*)、细胞液 pH 值及花色素的成分及含量进行测定。结果表明,细胞液 pH 值以红色花瓣最低(5.07),粉、白、黄色花瓣次之,蓝色花瓣最高(6.33)。红、粉、蓝色品种花瓣中花青苷含量较高,白色和黄色品种花瓣中类黄酮含量最高。同时花瓣细胞液 pH 值与蓝色品种特有的 5 种花青苷(飞燕草素 3–葡萄糖苷、报春色素苷、矮牵牛–7–甲氧基–3,5–二葡萄糖苷、锦葵素–3–葡糖苷和报春色素–4’–丙二酸酯)的含量呈显著正相关。红、粉和蓝色品种花瓣花色素提取液的颜色随着外界缓冲液 pH 的增大而逐渐变浅,并最终变 为黄色;在可见光区的最大吸收波长发生红移。通过分析可能调控欧洲报春 pH 的 6 个基因表达量及其与花瓣细胞液 pH 值的相关性,发现 PvPH4 在蓝色花瓣中显著高表达,并与花瓣细胞液 pH 值呈显著正相关;其编码的蛋白质保守结构域是属于 Ca2+/H+逆向转运超家族(ChaAsuperfamily)的 caca2,并且蛋白质三级结构建模显示其与液泡钙离子转运体相似度最高(39%)。因此推测 PvPH4 通过编码 Ca2+/H+转运跨膜蛋白,影响花瓣细胞液 pH 值,进而影响花色。

关键词: 欧洲报春, 花色, 花青素, 细胞液 pH, 基因表达, PvPH4

Abstract: To study the mechanism of cell sap pH on flower color formation in Primula vulgaris,petals of pink,red,white,yellow and blue were used as materials. The flower color indices were measured using CIE L*a*b* system,and the cell sap pH values in different colored petals were measured by planar pH meter. Furthermore,the composition and content of anthocyanin,flavonoids and carotenoid were assessed qualitatively and quantitatively by UPLC. And then,the color change and absorbance change of cell sap in different external buffer solutions were observed using UV spectrophotometer. The results showed that cell sap pH value was the lowest in red(5.07),followed by pink,white and yellow,and the highest in blue petal(6.33). The anthocyanidins were accumulated abundantly in pink,red and blue petals while the contents of flavonoids were the highest in white and yellow petals. Meanwhile,the cell sap pH values showed significantly positive correlations with the content of delphinidin-3-glucoside,hirsutin, petunidin-7- methoxy-3,5-diglucoside,malvidin-3-glucoside and hirsutin-4’-malonate,which all were specific in blue petals. Moreover,there was a bathochromic shift in the visible light region of the maximum absorption peak of pink,red and blue cultivars’ petal pigment extracting solution,and the color of pigment extracting solution of pink,red and blue petals was fading gradually with the increasing of buffer solution pH,and then turned into yellow. In addition,based on the results from expression profiles, the correlation between cell sap pH and gene expression,and the bioinformatics analysis,the relative expression of PvPH4 in blue petals was significantly higher than those in the other four colored petals. Furthermore,the relative expression of PvPH4 showed significantly positive correlation with cell sap pH. The conserved domain of PvPH4 was caca2,which belonged to ChaA superfamily. And the tertiary structure modeling of the protein showed that the similarity to the vacuolar calcium ion transporter was 39%. Thus,PvPH4 may play a role on flower color by encoding vacuolar calcium ion transporter to influence the petal cell sap pH.

Key words: Primula vulgaris, flower color, anthocyanin, cell sap pH, gene expression, PvPH4

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