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园艺学报 ›› 2019, Vol. 46 ›› Issue (8): 1495-1502.doi: 10.16420/j.issn.0513-353x.2019-0081

• 研究论文 • 上一篇    下一篇

菠菜雌雄同株性状的全基因组关联分析

汪豪英1,2,*,刘志远2,*,王晓武2,武 剑2,张合龙2,夏志兰1,徐兆生2,**,钱 伟2,**   

  1. 1湖南农业大学园艺园林学院,长沙 410128;2中国农业科学院蔬菜花卉研究所,北京 100081
  • 出版日期:2019-08-25 发布日期:2019-08-25
  • 基金资助:

    中国农业科学院科技创新工程项目(CAAS-ASTIP-IVFCAAS);国家自然科学基金项目(31872102);北京市科技计划项目(Z171100001517014);中国农业科学院基本科研业务费项目(1610102018004);国家重点研发计划项目(2018YFD0100805);农业部园艺作物生物学与种质创制重点实验室项目

Genome-wide Association Studies for Monoecism in Spinach

WANG Haoying1,2,*,LIU Zhiyuan2,*,WANG Xiaowu2,WU Jian2,ZHANG Helong2,XIA Zhilan1,XU Zhaosheng2,**,and QIAN Wei2,**   

  1. 1College of Horticulture and Landscape,Hunan Agricultural University,Changsha 410128,China;2Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences,Beijing 100081,China
  • Online:2019-08-25 Published:2019-08-25

摘要:

菠菜为典型的雌雄异株植物,少数植株表现为雌雄同株。为了挖掘控制菠菜雌雄同株的基因位点,对82份菠菜高代自交系的性型进行调查分析,同时进行测序深度为10×的重测序。重测序共获得了3 420 496个SNP,过滤后得到1 621 970个多态性高的SNP位点。利用高质量的SNP对菠菜雌雄同株的性状进行了全基因组关联分析(GWAS)。利用压缩混合线性模型(compress mixed linear model,cMLM)在菠菜4号染色体上检测到1个强关联区域。基于强关联信号SNP位点LD值(31.2 kb)的大小,将控制菠菜雌雄同株的基因Xm定位在64.6 kb的区间内。该范围内存在3个基因:Spo24600、Spo24601和Spo24602。该研究在关联区域内开发了KASP标记,可以有效用于菠菜雌雄同株的鉴定。

关键词: 菠菜, 全基因组, 关联分析, 雌雄同株

Abstract:

Spinach is a typical dioecious plant,but some varieties produce monoecious plant. In order to locate the genes controlling the monoecious traits of spinach,the sexual genotypes of 82 spinach inbred lines were investigated and analyzed. Each sample isolated from these accessions was re-sequenced with 10-fold sequencing depth,and a total of 3 420 496 SNPs were obtained. After filtration,1 621 970 high-quality polymorphic SNPs were acquired. The genome-wide association study(GWAS)of monoecious was performed by using 1 621 970 high-quality SNPs. The compress mixed linear model(cMLM)was used for genome-wide association analysis(GWAS)of monoecious traits in spinach using Genome Association and Prediction Integrated Tool(GAPIT)software. And a strong correlation region was detected on the chromosome 4. Based on the size of linkage disequilibrium block(31.2 kb),Xm gene was located in 64.6 kb region. Three genes,Spo24600,Spo24601 and Spo24602,were located in this region. In this study,KASP marker was developed in the correlation region,which can be used for the identification of monoecious spinach.

Key words: spinach, GWAS, monoecism

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