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园艺学报 ›› 2019, Vol. 46 ›› Issue (8): 1473-1485.doi: 10.16420/j.issn.0513-353x.2019-0011

• 研究论文 • 上一篇    下一篇

无核葡萄胚败育生理生化因子灰色关联分析

纪 薇1,2,3,*,郭荣荣1,2,3,*,王静波1,2,3,焦晓博1,2,3,闫 钊4,昌秦湘4,董志刚2,5,王跃进1,2,3,**   

  1. 1山西农业大学园艺学院,山西太谷 030801;2果树种质创制和利用山西省重点实验室,太原 030031;3农业部黄土高原作物基因资源与种质创制重点实验室,太原 030031;4太原学院,太原 030032;5山西省农业科学院果树研究所,山西太谷 030815
  • 出版日期:2019-08-25 发布日期:2019-08-25
  • 基金资助:

    山西省重点研发计划(指南)项目(201703D221014-4);山西省青年拔尖人才支持计划项目(2018);山西省高等学校优秀青年学术带头人(2017);山西农业大学青年拔尖创新人才支持计划项目

Grey Correlation Analysis of Physiological and Biochemical Factors in Embryo Abortion of Seedless Grape

JI Wei1,2,3,*,GUO Rongrong1,2,3,*,WANG Jingbo1,2,3,JIAO Xiaobo1,2,3,YAN Zhao4,CHANG Qinxiang4,DONG Zhigang2,5,and WANG Yuejin1,2,3,**   

  1. 1College of Horticulture,Shanxi Agricultural University,Taigu,Shanxi 030801,China;2Province Key Laboratory of Fruit Germplasm Development and Utilization,Taiyuan 030031,China;3Key Laboratory of Crop Gene Resources and Germplasm Enhancement on Loess Plateau,Ministry of Agriculture,Taiyuan 030031,China;4Taiyuan University,Taiyuan 030032,China;5 Pomology Institute,Shanxi Academy of Agricultural Sciences,Taigu,Shanxi 030815,China
  • Online:2019-08-25 Published:2019-08-25

摘要:

以无核葡萄‘无核翠宝’、‘丽红宝’和有核葡萄‘赤霞珠’(对照)为试材,观测其发育过程中果实和胚珠的形态学、生理学变化,利用主成分分析法对6个生理生化指标进行分析,结合灰色关联度分析,筛选出与无核葡萄胚败育相关的主要生理生化因子。结果表明,‘无核翠宝’和‘丽红宝’分别于花后 36 d和32 d胚珠畸形率达最高,开始败育。随着果实发育,3个葡萄品种胚珠中丙二醛(MDA)含量呈上升趋势;果肉中可溶性糖和可溶性蛋白含量均呈上升趋势,但在无核葡萄胚珠中先增后降,在败育时期达到最大;POD和CAT活性在两个无核品种中先升后降,在败育时期的胚珠中显著高于有核葡萄,而在果肉中显著低于有核葡萄;胚珠的SOD活性在无核葡萄败育前显著增加,在有核葡萄中则呈上升趋势。综合分析,无核葡萄果肉中可溶性糖、可溶性蛋白含量和胚珠中MDA、可溶性蛋白含量,SOD活性等生理指标为无核葡萄胚败育的主要影响因子,其中胚珠SOD活性与无核葡萄胚败育关联度最高,其次是果肉可溶性蛋白含量和胚珠数。本研究为今后无核葡萄胚挽救育种采样时期的确定提供了理论指导。

关键词: 葡萄, 无核, 胚败育, 生理生化因子, 灰色关联度

Abstract:

This study was to determine the major physiological and biochemical factors affecting embryo abortion in seedless grape. Two seedless grape varieties,‘Wuhe Cuibao’and‘Lihongbao’,and one seeded variety,‘Cabernet Sauvignon’(control),were used. Morphological index,physiological and biochemical change in pulp and ovule were observed and measured at various days after full bloom. Major parameters related to embryo abortion were determined using principal component analysis and grey correlation analysis. The results showed that the rate of ovule deformity reached the highest at 36 days after full bloom for‘Wuhe Cuibao’and at 32 days after full bloom for‘Lihongbao’,at those days the ovules began to abort. In the three grape varieties,the content of MDA in ovule and the content of soluble sugar and soluble protein in pulp increased with fruit development. In both seedless varieties,soluble sugar and protein in ovule reached the highest content during abortion,and then decreased. The activities of POD and CAT in seedless grapes first increased and then decreased. During abortion,ovule POD and CAT activities were significantly higher in the seedless varieties compared to the seeded variety,but the converse was also true for pulp POD and CAT activities. In the seedless varieties,the activity of SOD in ovule increased significantly before abortion; in the seeded variety,ovule SOD activity increased slowly with time. The analysis demonstrated that in seedless grape the major factors affecting embryo abortion are pulp soluble sugar and protein,ovule MDA,ovule soluble protein and ovule SOD. Among them,ovule SOD activity showed the best correlation with embryo abortion,followed by pulp soluble protein content and ovule number. This study could provide the theoretical guidance for determining sampling time in embryo-rescue breeding programs for seedless grape.

Key words: grape, seedless, embryo abortion, physiological and biochemical factors, grey correlation

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