园艺学报 ›› 2019, Vol. 46 ›› Issue (7): 1351-1358.doi: 10.16420/j.issn.0513-353x.2018-1031

• 研究论文 • 上一篇    下一篇


付 晓,苏江硕,李媛媛,张 飞,房伟民,陈素梅,陈发棣,管志勇*   

  1. 南京农业大学园艺学院,农业部景观农业重点实验室,南京 210095
  • 出版日期:2019-07-25 发布日期:2019-07-25
  • 基金资助:

Genetic Variation and QTL Mapping for Aphid Resistance in an F1 Population of Chrysanthemum

FU Xiao,SU Jiangshuo,LI Yuanyuan,ZHANG Fei,FANG Weimin,CHEN Sumei,CHEN Fadi,and GUAN Zhiyong*   

  1. College of Horticulture,Nanjing Agricultural University,Nanjing 210095,China
  • Online:2019-07-25 Published:2019-07-25

摘要: 以菊花(Chrysanthemum morifolium Ramat.)‘南农雪峰’(抗蚜虫)ב蒙白’(感蚜虫)的F1代为材料,通过温室自然感蚜方法调查苗期蚜害指数,分析抗蚜性的遗传变异,并开展QTL定位研究。结果表明:菊花抗蚜性在F1群体中广泛分离,苗期蚜害指数在0.20 ~ 0.92之间,变异系数为36.73%,中亲优势和双向超亲分离现象普遍存在,基本符合双峰偏态分布,为多基因控制的数量性状。主基因 + 多基因混合遗传模型分析表明,该F1群体菊花抗蚜性的遗传符合由两对主基因控制的B-2模型,主基因表现为加性—显性效应,主基因遗传率为0.91。基于复合区间作图法的QTL分析共检测到4个与菊花抗蚜性显著相关的QTL(ArX3、ArX4、ArX30和 ArM9),主要分布在‘南农雪峰’遗传图谱的X3、X4、X30连锁群和‘蒙白’遗传图谱的M9连锁群上,LOD值介于2.40 ~ 3.52之间,单个QTL可以解释抗蚜性变异的5.90% ~ 9.38%,均为微效QTL。

关键词: 菊花, 抗蚜性, 遗传变异, 主基因 + 多基因, QTL定位

Abstract: The performance of aphid resistance,quantified by aphid damage index,was evaluated for the 113 randomly selected F1 lines derived from‘Nannong Xuefeng’בMonalisa’at seedling stages under greenhouse condition,and the genetic variation and QTL responsible for aphid resistance were examined in the present study. The result showed that aphid damage index ranged between 0.20–0.92 and distributed normally with a coefficient of variation of 36.73%,indicative of the quantitative nature;in addition,transgressive segregation was observed in both directions,which basically accords with the trend of bimodality. Major gene plus polygene inheritance model analysis figured out the inheritance of aphid resistance fitted B-2 model that was governed by two major genes showing additive-dominant effects,and the heritability of the major genes was calculated at 0.91. QTL analysis based on the composite interval mapping method detected four aphid-resistance-associated QTLs,namely ArX3,ArX4,ArX30 and ArM9,harboring on the linkage group X3,X4 and X30 of‘Nannong Xuefeng’map and M9 of‘Monolisa’map, with a LOD value of 2.40–3.52. The additive effect of the four QTLs varied in a range of–0.16–0.18,and the individually explained phenotypic variation ranged from 5.90% to 9.38%,therefore the identified QTL should be minor genes.

Key words: chrysanthemum, aphid resistance, genetic variation, major gene plus polygene, quantitative trait loci(QTL)