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园艺学报 ›› 2019, Vol. 46 ›› Issue (6): 1163-1171.doi: 10.16420/j.issn.0513-353x.2018-0998

• 研究报告 • 上一篇    下一篇

马铃薯StRcd1基因克隆及其在非生物胁迫下的表达分析

陈英平1,王 芳1,2,*,王 舰1,2,孙海宏1,2,贺苗苗1,2   

  1. 1青海大学,西宁 810016;2青海大学青藏高原生物技术教育部重点实验室,青海省农林科学院,省部共建三江源生态与高原农牧业国家重点实验室,青海省马铃薯育种重点实验室,西宁 810016
  • 出版日期:2019-06-25 发布日期:2019-06-25
  • 基金资助:

    国家自然科学基金项目(31660417);国家现代农业产业技术体系建设专项资金项目(CARS-9);青海省科技厅科技合作专项(2018-HZ-802)

Cloning of Potato StRcd1 and Its Expression Analysis Under Abiotic Stress

CHEN Yingping1,WANG Fang1,2,*,WANG Jian1,2,SUN Haihong1,2,and HE Miaomiao1,2   

  1. 1Qinghai University,Xining 810016,China;2Key Laboratory of Qinghai-Tibet Plateau Biotechnology Ministry of Education,Qinghai Academy of Agriculture and Forestry Science,State Key Laboratory of Sanjiangyuan Ecology and Plateau Agriculture and Animal Husbandry,Key Laboratory of Potato Breeding in Qinghai Province,Xining 810016,China
  • Online:2019-06-25 Published:2019-06-25

摘要:

Rcd1(细胞分化必需因子)蛋白是CCR4-NOT蛋白复合体的主要亚基,是真核生物中发现的最保守的蛋白质之一,其同源物在多种分化组织中表达。为了解Rcd1蛋白在马铃薯应答非生物胁迫中的功能,以马铃薯品种‘青薯9号’为材料,克隆了StRcd1基因,分析了在模拟干旱胁迫和盐胁迫下该基因的表达模式。结果表明,StRcd1的全长为1 223 bp,开放阅读框为897 bp,编码298个氨基酸,亚细胞定位于细胞核。StRcd1在马铃薯块茎中高表达,响应干旱和盐胁迫应答,但不同组织中表达模式存在差异,在根部12 h的盐胁迫和24 h的干旱胁迫下表达量最高,分别为对照的5.96倍和9.37倍。推测StRcd1在马铃薯响应干旱和盐胁迫应答中发挥重要作用。

关键词: 马铃薯, 细胞分化必需因子(Rcd1), 基因克隆, 表达分析

Abstract:

The Rcd1 protein is the major subunit of the CCR4-NOT protein complex and is one of the most conserved proteins found in eukaryotes,and its homologs are expressed in a variety of differentiated tissues. In order to understand the function of Rcd1 protein in response to abiotic stress in potato,the StRcd1 gene was cloned from potato cultivar‘Qingshu 9’. The expression pattern of the gene under simulated drought stress and salt stress was analyzed. The results showed that the full length of StRcd1 was 1 223 bp,the open reading frame was 897 bp,encoding 298 amino acids,and the subcellular cells were located in the nucleus. StRcd1 was highly expressed in potato tubers and responded to drought and salt stress,but the expression patterns in different tissues were different. The expression of the roots was the highest under the salt stress of 12 h and drought stress of 24 h,which were 5.96 times and 9.37 times of the control,respectively. It is speculated that StRcd1 plays an important role in response to drought and salt stress in potato.

Key words: potato, required cell differentiation 1, gene clone;expression analysis

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